Next Article in Journal
Biological and Genomic Characterization of a Novel Jumbo Bacteriophage, vB_VhaM_pir03 with Broad Host Lytic Activity against Vibrio harveyi
Previous Article in Journal
Porcine Circovirus 2 Genotypes, Immunity and Vaccines: Multiple Genotypes but One Single Serotype
Previous Article in Special Issue
Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome
Article

Piscine Orthoreovirus-1 Isolates Differ in Their Ability to Induce Heart and Skeletal Muscle Inflammation in Atlantic Salmon (Salmo salar)

1
Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 0454 Oslo, Norway
2
Norwegian Veterinary Institute, 0454 Oslo, Norway
3
FishVetGroup, 0275 Oslo, Norway
4
Nofima, 1430 Ås, Norway
5
PatoGen, 6002 Ålesund, Norway
6
VESO Vikan, 7810 Namsos, Norway
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(12), 1050; https://doi.org/10.3390/pathogens9121050
Received: 21 November 2020 / Revised: 10 December 2020 / Accepted: 11 December 2020 / Published: 14 December 2020
Piscine orthoreovirus 1 (PRV-1) is the causative agent of heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). The virus is widespread in Atlantic salmon and was present in Norway long before the first description of HSMI in 1999. Furthermore, in Canada the virus is prevalent in farmed Atlantic salmon but HSMI is not and Canadian isolates have failed to reproduce HSMI experimentally. This has led to the hypothesis that there are virulence differences between PRV-1 isolates. In this study we performed a dose standardized challenge trial, comparing six PRV-1 isolates, including two Norwegian field isolates from 2018, three historical Norwegian isolates predating the first report of HSMI and one Canadian isolate. The Norwegian 2018 isolates induced lower viral protein load in blood cells but higher plasma viremia. Following peak replication in blood, the two Norwegian 2018 isolates induced histopathological lesions in the heart consistent with HSMI, whereas all three historical Norwegian and the Canadian isolates induced only mild cardiac lesions. This is the first demonstration of virulence differences between PRV-1 isolates and the phenotypic differences are linked to viral proteins encoded by segment S1, M2, L1, L2 and S4. View Full-Text
Keywords: PRV-1; heart and skeletal muscle inflammation; virulence; Atlantic salmon PRV-1; heart and skeletal muscle inflammation; virulence; Atlantic salmon
Show Figures

Figure 1

MDPI and ACS Style

Wessel, Ø.; Hansen, E.F.; Dahle, M.K.; Alarcon, M.; Vatne, N.A.; Nyman, I.B.; Soleim, K.B.; Dhamotharan, K.; Timmerhaus, G.; Markussen, T.; Lund, M.; Aanes, H.; Devold, M.; Inami, M.; Løvoll, M.; Rimstad, E. Piscine Orthoreovirus-1 Isolates Differ in Their Ability to Induce Heart and Skeletal Muscle Inflammation in Atlantic Salmon (Salmo salar). Pathogens 2020, 9, 1050. https://doi.org/10.3390/pathogens9121050

AMA Style

Wessel Ø, Hansen EF, Dahle MK, Alarcon M, Vatne NA, Nyman IB, Soleim KB, Dhamotharan K, Timmerhaus G, Markussen T, Lund M, Aanes H, Devold M, Inami M, Løvoll M, Rimstad E. Piscine Orthoreovirus-1 Isolates Differ in Their Ability to Induce Heart and Skeletal Muscle Inflammation in Atlantic Salmon (Salmo salar). Pathogens. 2020; 9(12):1050. https://doi.org/10.3390/pathogens9121050

Chicago/Turabian Style

Wessel, Øystein, Elisabeth F. Hansen, Maria K. Dahle, Marta Alarcon, Nina A. Vatne, Ingvild B. Nyman, Karen B. Soleim, Kannimuthu Dhamotharan, Gerrit Timmerhaus, Turhan Markussen, Morten Lund, Håvard Aanes, Magnus Devold, Makoto Inami, Marie Løvoll, and Espen Rimstad. 2020. "Piscine Orthoreovirus-1 Isolates Differ in Their Ability to Induce Heart and Skeletal Muscle Inflammation in Atlantic Salmon (Salmo salar)" Pathogens 9, no. 12: 1050. https://doi.org/10.3390/pathogens9121050

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop