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Open AccessArticle

Immunomodulatory Effects of Recombinant Mycobacterium smegmatis Expressing Antigen-85B Epitopes in Infected J774A.1 Murine Macrophages

1
School of Biomedicine, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Kuala Nerus 21300, Terengganu, Malaysia
2
School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia
*
Authors to whom correspondence should be addressed.
Pathogens 2020, 9(12), 1000; https://doi.org/10.3390/pathogens9121000
Received: 22 September 2020 / Revised: 15 November 2020 / Accepted: 23 November 2020 / Published: 29 November 2020
(This article belongs to the Special Issue Tuberculosis Epidemiology and Control in Multi-Host Systems)
Tuberculosis (TB) causes more than 1.5 million deaths each year, remaining a significant global health problem. Mycobacterium smegmatis (M. smegmatis) and Mycobacterium tuberculosis (M. tuberculosis) share features, which support the use of the former use in new generation TB vaccine development. In a previous study, the specific humoral and cellular immunogenicity of a recombinant M. smegmatis strain expressing epitopes from M. tuberculosis Ag85B protein (rMs064), was demonstrated in mice. In the current study, the immunomodulatory capacity of rMs064 was determined in a J774A.1 murine macrophage cell line. To determine the immunomodulatory effect of rMs064 in J774A.1 macrophages, the expression of inducible nitric oxide synthase (iNOS) and production of nitric oxide (NO) was evaluated. The expression of activation surface markers (MHC-II, CD40, CD80 and CD86) and the production of cytokines (IL-1β, TNF-α, IL-12p70 and IL-6) was also determined in rMs064 infected J774A.1 macrophages. Our findings showed the ability of rMs064 to induce substantial increases in macrophage activation markers expression; MHC class II and CD40, compared with M. smegmatis transformed with the empty vector (rMs012) and uninfected cells. rMs064 induced significant increases in IL-12p70 compared to uninfected cells. The expression of iNOS and CD86, and the production of IL-1β, and TNF-α were increased in rMs064 and rMs012, compared to uninfected cells. rMs064 demonstrated its immunomodulatory ability by stimulating the innate immune response, which supports its further evaluation as a TB vaccine candidate. View Full-Text
Keywords: tuberculosis; BCG; mycobacteria; M. tuberculosis; M. smegmatis; antigen-85B tuberculosis; BCG; mycobacteria; M. tuberculosis; M. smegmatis; antigen-85B
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MDPI and ACS Style

Kadir, N.-A.; Acosta, A.; Sarmiento, M.E.; Norazmi, M.-N. Immunomodulatory Effects of Recombinant Mycobacterium smegmatis Expressing Antigen-85B Epitopes in Infected J774A.1 Murine Macrophages. Pathogens 2020, 9, 1000. https://doi.org/10.3390/pathogens9121000

AMA Style

Kadir N-A, Acosta A, Sarmiento ME, Norazmi M-N. Immunomodulatory Effects of Recombinant Mycobacterium smegmatis Expressing Antigen-85B Epitopes in Infected J774A.1 Murine Macrophages. Pathogens. 2020; 9(12):1000. https://doi.org/10.3390/pathogens9121000

Chicago/Turabian Style

Kadir, Nur-Ayuni; Acosta, Armando; Sarmiento, Maria E.; Norazmi, Mohd-Nor. 2020. "Immunomodulatory Effects of Recombinant Mycobacterium smegmatis Expressing Antigen-85B Epitopes in Infected J774A.1 Murine Macrophages" Pathogens 9, no. 12: 1000. https://doi.org/10.3390/pathogens9121000

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