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The Role of Apoptin in Chicken Anemia Virus Replication
Article

Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection

1
United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu City 501-1193, Japan
2
Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan
3
Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Trau Quy Town, Gia Lam, Hanoi 131000, Vietnam
4
Central Veterinary Medicine JSC No. 5, Ha Binh Phuong Industrial Zone, Thuong Tin, Hanoi 131000, Vietnam
5
Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(10), 842; https://doi.org/10.3390/pathogens9100842
Received: 5 September 2020 / Revised: 12 October 2020 / Accepted: 13 October 2020 / Published: 15 October 2020
(This article belongs to the Special Issue Chicken Anaemia Virus Infection)
Persistent infection of chicken anemia virus (CAV) in chickens has been suspected to result in immunosuppression and exogenous virus contamination within vaccine production. However, no direct evidence for persistent CAV infection has thus far been obtained. In this study, we aimed to establish an in vitro model of persistent CAV infection. CAV-infected MDCC-MSB1 (MSB1) cells, a Marek’s disease virus-transformed continuous cell line, were cultured in the presence of both CAV and CAV neutralizing antibody (NA). Cell viability, expression of viral antigens, viral DNA, and recovery of CAV were examined by acridine orange/propidium iodide staining, immunofluorescence measurement, real-time PCR, and viral isolation, respectively. The results indicated that CAV was maintained and possibly replicated in CAV-infected cells cultured in the presence of NA, without affecting host cell viability. It was also shown that persistently infectious CAV induced cell death again after removing NA. The persistent infection of CAV in MSB1 cells was not related to viral gene mutation. In summary, we have herein established a novel model of persistent CAV infection in MSB1 cells cultured in the presence of NA. View Full-Text
Keywords: chicken anemia virus; in vitro model; neutralizing antibody; persistent infection chicken anemia virus; in vitro model; neutralizing antibody; persistent infection
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MDPI and ACS Style

Van Dong, H.; Tran, G.T.H.; Trinh, D.Q.; Takeda, Y.; Ogawa, H.; Imai, K. Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection. Pathogens 2020, 9, 842. https://doi.org/10.3390/pathogens9100842

AMA Style

Van Dong H, Tran GTH, Trinh DQ, Takeda Y, Ogawa H, Imai K. Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection. Pathogens. 2020; 9(10):842. https://doi.org/10.3390/pathogens9100842

Chicago/Turabian Style

Van Dong, Hieu; Tran, Giang T.H.; Trinh, Dai Q.; Takeda, Yohei; Ogawa, Haruko; Imai, Kunitoshi. 2020. "Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection" Pathogens 9, no. 10: 842. https://doi.org/10.3390/pathogens9100842

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