New Species of the Genus Curvularia: C. tamilnaduensis and C. coimbatorensis from Fungal Keratitis Cases in South India
by
Noémi Kiss 1, Mónika Homa 1,2, Palanisamy Manikandan 3,4
, Arumugam Mythili 5, Krisztina Krizsán 6, Rajaraman Revathi 7, Mónika Varga 1, Tamás Papp 1,2, Csaba Vágvölgyi 1, László Kredics 1,* and Sándor Kocsubé 1,*
Noémi Kiss 1, Mónika Homa 1,2, Palanisamy Manikandan 3,4, Arumugam Mythili 5, Krisztina Krizsán 6, Rajaraman Revathi 7, Mónika Varga 1, Tamás Papp 1,2, Csaba Vágvölgyi 1, László Kredics 1,* and Sándor Kocsubé 1,*
1
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary
2
MTA-SZTE “Lendület” Fungal Pathogenicity Mechanisms Research Group, 6726 Szeged, Hungary
3
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi Arabia
4
Greenlink Analytical and Research Laboratory India Private Ltd., Coimbatore, Tamil Nadu 641014, India
5
Department of Microbiology, Dr. G.R. Damodaran College of Science, Coimbatore, Tamil Nadu 641014, India
6
Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, 6726 Szeged, Hungary
7
Aravind Eye Hospital and Postgraduate Institute of Ophthalmology, Coimbatore, Tamil Nadu 641014, India
*
Authors to whom correspondence should be addressed.
Pathogens 2020, 9(1), 9; https://doi.org/10.3390/pathogens9010009
Received: 6 December 2019 / Revised: 17 December 2019 / Accepted: 18 December 2019 / Published: 20 December 2019
(This article belongs to the Section Human Pathogens)
Members of the genus Curvularia are melanin-producing dematiaceous fungi of increasing clinical importance as causal agents of both local and invasive infections. This study contributes to the taxonomical and clinical knowledge of this genus by describing two new Curvularia species based on isolates from corneal scrapings of South Indian fungal keratitis patients. The phylogeny of the genus was updated based on three phylogenetic markers: the internal transcribed spacer (ITS) region of the ribosomal RNA gene cluster as well as fragments of the glyceraldehyde-3-phosphate dehydrogenase (gpdh) and translation elongation factor 1-α (tef1α) genes. The maximum likelihood phylogenetic tree constructed from the alignment of the three concatenated loci revealed that the examined isolates are representing two new, yet undescribed, Curvularia species. Examination of colony and microscopic morphology revealed differences between the two species as well as between the new species and their close relatives. The new species were formally described as Curvularia tamilnaduensis N. Kiss & S. Kocsubé sp. nov. and Curvularia coimbatorensis N. Kiss & S. Kocsubé sp. nov. Antifungal susceptibility testing by the broth microdilution method of CLSI (Clinical & Laboratory Standards Institute) revealed that the type strain of C. coimbatorensis is less susceptible to a series of antifungals than the C. tamilnaduensis strains.
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Keywords:
Curvularia; keratitis; taxonomy; antifungal susceptibility; Curvularia coimbatorensis; Curvularia tamilnaduensis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Kiss, N.; Homa, M.; Manikandan, P.; Mythili, A.; Krizsán, K.; Revathi, R.; Varga, M.; Papp, T.; Vágvölgyi, C.; Kredics, L.; Kocsubé, S. New Species of the Genus Curvularia: C. tamilnaduensis and C. coimbatorensis from Fungal Keratitis Cases in South India. Pathogens 2020, 9, 9.
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