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Open AccessArticle

High Prevalence of CTX-M Type Extended-Spectrum Beta-Lactamase Genes and Detection of NDM-1 Carbapenemase Gene in Extraintestinal Pathogenic Escherichia coli in Cuba

1
Department of Bacteriology-Mycology, Pedro Kourí Institute of Tropical Medicine, Havana 17100, Cuba
2
Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan
3
Microbiology Department, Héroes de Baire Hospital, Isla de La Juventud 25100, Cuba
4
Microbiology Department, Enrique Cabrera hospital, Havana 10400, Cuba
5
Microbiology Department, Juan Bruno Zayas Hospital, Santiago de Cuba 90100, Cuba
6
Ramón y Cajal Hospital, 28002 Madrid, Spain
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(1), 65; https://doi.org/10.3390/pathogens9010065
Received: 23 December 2019 / Revised: 12 January 2020 / Accepted: 14 January 2020 / Published: 16 January 2020
(This article belongs to the Section Human Pathogens)
Increase of extraintestinal pathogenic Escherichia coli (ExPEC) showing resistance to beta-lactams is a major public health concern. This study was conducted as a first molecular epidemiological study on ExPEC in Cuba, regarding prevalence of extended-spectrum beta-lactamases (ESBLs) and carbapenemase genes. A total of 306 ExPEC isolates collected in medical institutions in 16 regions in Cuba (2014–2018) were analyzed for their genotypes and presence of genes encoding ESBL, carbapenemase, plasmid-mediated quinolone resistance (PMQR) determinants by PCR and sequencing. The most common phylogenetic group of ExPEC was B2 (49%), followed by D (23%), A (21%), and B1 (7%). Among ESBL genes detected, blaCTX-M was the most common and detected in 61% of ExPEC, with blaCTX-M-15 being dominant and distributed to all the phylogenetic groups. NDM-1 type carbapenemase gene was identified in two isolates of phylogenetic group B1-ST448. Phylogenetic group B2 ExPEC belonged to mostly ST131 (or its single-locus variant) with O25b allele, harboring blaCTX-M-27, and included an isolate of emerging type ST1193. aac (6’)-Ib-cr was the most prevalent PMQR gene (40.5%), being present in 54.5% of CTX-M-positive isolates. These results indicated high prevalence of CTX-M genes and the emergence of NDM-1 gene among recent ExPEC in Cuba, depicting an alarming situation. View Full-Text
Keywords: extraintestinal pathogenic E. coli (ExPEC); extended-spectrum beta-lactamase (ESBL); carbapenemase; NDM-1; Cuba extraintestinal pathogenic E. coli (ExPEC); extended-spectrum beta-lactamase (ESBL); carbapenemase; NDM-1; Cuba
MDPI and ACS Style

Quiñones, D.; Aung, M.S.; Carmona, Y.; González, M.K.; Pereda, N.; Hidalgo, M.; Rivero, M.; Zayas, A.; del Campo, R.; Urushibara, N.; Kobayashi, N. High Prevalence of CTX-M Type Extended-Spectrum Beta-Lactamase Genes and Detection of NDM-1 Carbapenemase Gene in Extraintestinal Pathogenic Escherichia coli in Cuba. Pathogens 2020, 9, 65.

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