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Open AccessArticle

Toxoplasma gondii Recombinant antigen AMA1: Diagnostic Utility of Protein Fragments for the Detection of IgG and IgM Antibodies

1
Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland
2
Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Łódź, Poland
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(1), 43; https://doi.org/10.3390/pathogens9010043
Received: 17 December 2019 / Revised: 3 January 2020 / Accepted: 3 January 2020 / Published: 5 January 2020
(This article belongs to the Section Human Pathogens)
Toxoplasma gondii is an important zoonotic protozoan that infects a wide variety of vertebrates as intermediate hosts. For this reason, the diagnosis of this disease is very important and requires continuous improvement. One possibility is to use recombinant antigens in serological tests. Apical membrane antigen 1 (AMA1), a protein located in specific secretory organelles (micronemes) of T. gondii, is very interesting in regard to its potential diagnostic utility. In the present study, we attempted to identify a fragment of the AMA1 protein with a high sensitivity and specificity for the serological diagnosis of human toxoplasmosis. The full-length AMA1 and two different fragments (AMA1N and AMA1C) were produced using an Escherichia coli expression system. After purification by metal affinity chromatography, recombinant proteins were tested for their utility as antigens in enzyme-linked immunosorbent assays (ELISAs) for the detection of IgG and IgM anti-T. gondii antibodies in human and mouse immune sera. Our data demonstrate that the full-length AMA1 recombinant antigen (corresponding to amino acid residues 67–569 of the native protein) has a better diagnostic potential than its N- or C-terminal fragments. This recombinant protein strongly interacts with specific anti-T. gondii IgG (99.4%) and IgM (80.0%) antibodies, and may be used for developing new tools for diagnostics of toxoplasmosis. View Full-Text
Keywords: ELISA; serological detection; apical membrane antigen 1 (AMA1); IgG; IgM; Toxoplasma gondii ELISA; serological detection; apical membrane antigen 1 (AMA1); IgG; IgM; Toxoplasma gondii
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MDPI and ACS Style

Ferra, B.; Holec-Gąsior, L.; Gatkowska, J.; Dziadek, B.; Dzitko, K. Toxoplasma gondii Recombinant antigen AMA1: Diagnostic Utility of Protein Fragments for the Detection of IgG and IgM Antibodies. Pathogens 2020, 9, 43.

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