Next Article in Journal
Surfactant Protein A Impairs Genital HPV16 Pseudovirus Infection by Innate Immune Cell Activation in A Murine Model
Next Article in Special Issue
Bactericidal Effect of Clove Oil against Multidrug-Resistant Streptococcus suis Isolated from Human Patients and Slaughtered Pigs
Previous Article in Journal
Inactivation of Classical Swine Fever Virus in Porcine Serum Samples Intended for Antibody Detection
Previous Article in Special Issue
From Stable to Lab—Investigating Key Factors for Sudden Deaths Caused by Streptococcus suis
Open AccessArticle

The Impact of SsPI-1 Deletion on Streptococcus suis Virulence

by Yan Zhao 1,†, Gang Li 1,†, Xin-Yue Yao 2,†, Shu-Guang Lu 1, Jing Wang 1, Xiao-Dong Shen 3,* and Ming Li 1,*
1
Department of Microbiology, College of Basic Medical Sciences, Army Medical University (Third Military Medical University), Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Chongqing 400038, China
2
Jinling Hospital Research Institute of Clinical Laboratory Medicine, Nanjing University, School of Medicine, Nanjing 210002, China
3
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Army Medical University (Third Military Medical University), Chongqing 400038, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this study.
Pathogens 2019, 8(4), 287; https://doi.org/10.3390/pathogens8040287
Received: 7 November 2019 / Revised: 3 December 2019 / Accepted: 4 December 2019 / Published: 6 December 2019
(1) Background: Streptococcus suis is an important zoonotic pathogen that infects pigs and can occasionally cause life-threatening systemic infections in humans. Two large-scale outbreaks of streptococcal toxic shock-like syndrome in China suggest that the pathogenicity of S. suis has been changing in recent years. Genetic analysis revealed the presence of a chromosomal pathogenicity island (PAI) designated SsPI-1 in Chinese epidemic S. suis strains. The purpose of this study is to define the role of SsPI-1 in the virulence of S. suis. (2) Methods: A SsPI-1 deletion mutant was compared to the wild-type strain regarding the ability to attach to epithelial cells, to cause host disease and mortality, and to stimulate host immune response in experimental infection of piglets. (3) Results: Deletion of SsPI-1 significantly reduces adherence of S. suis to epithelial cells and abolishes the lethality of the wild-type strain in piglets. The SsPI-1 mutant causes no significant pathological lesions and exhibits an impaired ability to induce proinflammatory cytokine production. (4) Conclusions: Deletion of the SsPI-1 PAI attenuates the virulence of this pathogen. We conclude that SsPI-1 is a critical contributor to the evolution of virulence in epidemic S. suis. View Full-Text
Keywords: Streptococcus suis; pathogenicity island; deletion; virulence Streptococcus suis; pathogenicity island; deletion; virulence
Show Figures

Figure 1

MDPI and ACS Style

Zhao, Y.; Li, G.; Yao, X.-Y.; Lu, S.-G.; Wang, J.; Shen, X.-D.; Li, M. The Impact of SsPI-1 Deletion on Streptococcus suis Virulence. Pathogens 2019, 8, 287.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop