Next Article in Journal
Com1 as a Promising Protein for the Differential Diagnosis of the Two Forms of Q Fever
Previous Article in Journal
Electrocardiography Abnormalities in Macaques after Infection with Encephalitic Alphaviruses

LmxM.22.0250-Encoded Dual Specificity Protein/Lipid Phosphatase Impairs Leishmania mexicana Virulence In Vitro

Life Sciences Research Centre, Faculty of Science, University of Ostrava, 71000 Ostrava, Czech Republic
Department of Parasitology, Faculty of Science, Charles University, 12844 Prague, Czech Republic
Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 37005 České Budejovice (Budweis), Czech Republic
Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia
Zoological Institute of the Russian Academy of Sciences, 199034 St. Petersburg, Russia
University of South Bohemia, Faculty of Sciences, 37005 České Budejovice (Budweis), Czech Republic
Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov University, 119435 Moscow, Russia
Author to whom correspondence should be addressed.
Pathogens 2019, 8(4), 241;
Received: 23 October 2019 / Revised: 8 November 2019 / Accepted: 13 November 2019 / Published: 17 November 2019
Protein phosphorylation/dephosphorylation is an important regulatory mechanism that controls many key physiological processes. Numerous pathogens successfully use kinases and phosphatases to internalize, replicate, and survive, modifying the host′s phosphorylation profile or signal transduction pathways. Multiple phosphatases and kinases from diverse bacterial pathogens have been implicated in human infections before. In this work, we have identified and characterized the dual specificity protein/lipid phosphatase LmDUSP1 as a novel virulence factor governing Leishmania mexicana infection. The LmDUSP1-encoding gene (LmxM.22.0250 in L. mexicana) has been acquired from bacteria via horizontal gene transfer. Importantly, its orthologues have been associated with virulence in several bacterial species, such as Mycobacterium tuberculosis and Listeria monocytogenes. Leishmania mexicana with ablated LmxM.22.0250 demonstrated severely attenuated virulence in the experimental infection of primary mouse macrophages, suggesting that this gene facilitates Leishmania pathogenicity in vertebrates. Despite significant upregulation of LmxM.22.0250 expression in metacyclic promastigotes, its ablation did not affect the ability of mutant cells to differentiate into virulent stages in insects. It remains to be further investigated which specific biochemical pathways involve LmDUSP1 and how this facilitates the parasite′s survival in the host. One of the interesting possibilities is that LmDUSP1 may target host′s substrate(s), thereby affecting its signal transduction pathways. View Full-Text
Keywords: LmDUSP1; virulence factor; Leishmania infection LmDUSP1; virulence factor; Leishmania infection
Show Figures

Figure 1

MDPI and ACS Style

Kraeva, N.; Leštinová, T.; Ishemgulova, A.; Majerová, K.; Butenko, A.; Vaselek, S.; Bespyatykh, J.; Charyyeva, A.; Spitzová, T.; Kostygov, A.Y.; Lukeš, J.; Volf, P.; Votýpka, J.; Yurchenko, V. LmxM.22.0250-Encoded Dual Specificity Protein/Lipid Phosphatase Impairs Leishmania mexicana Virulence In Vitro. Pathogens 2019, 8, 241.

AMA Style

Kraeva N, Leštinová T, Ishemgulova A, Majerová K, Butenko A, Vaselek S, Bespyatykh J, Charyyeva A, Spitzová T, Kostygov AY, Lukeš J, Volf P, Votýpka J, Yurchenko V. LmxM.22.0250-Encoded Dual Specificity Protein/Lipid Phosphatase Impairs Leishmania mexicana Virulence In Vitro. Pathogens. 2019; 8(4):241.

Chicago/Turabian Style

Kraeva, Natalya, Tereza Leštinová, Aygul Ishemgulova, Karolina Majerová, Anzhelika Butenko, Slavica Vaselek, Julia Bespyatykh, Arzuv Charyyeva, Tatiana Spitzová, Alexei Yu. Kostygov, Julius Lukeš, Petr Volf, Jan Votýpka, and Vyacheslav Yurchenko. 2019. "LmxM.22.0250-Encoded Dual Specificity Protein/Lipid Phosphatase Impairs Leishmania mexicana Virulence In Vitro" Pathogens 8, no. 4: 241.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop