Non-Specific Lipid Transfer Proteins in Triticum kiharae Dorof. et Migush.: Identification, Characterization and Expression Profiling in Response to Pathogens and Resistance Inducers

Odintsova, Tatyana I.; Slezina, Marina P.; Istomina, Ekaterina A.; Korostyleva, Tatyana V.; Kovtun, Alexey S.; Kasianov, Artem S.; Shcherbakova, Larisa A.; Kudryavtsev, Alexander M.

doi:10.3390/pathogens8040221

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Non-Specific Lipid Transfer Proteins in Triticum kiharae Dorof. et Migush.: Identification, Characterization and Expression Profiling in Response to Pathogens and Resistance Inducers

Tatyana I. Odintsova

^1,*

Marina P. Slezina

Ekaterina A. Istomina

Tatyana V. Korostyleva

¹,

Alexey S. Kovtun

²,

Artem S. Kasianov

^3,4,

Larisa A. Shcherbakova

⁵ and

Alexander M. Kudryavtsev

⁶

Laboratory of Molecular-Genetic Bases of Plant Immunity, Vavilov Institute of General Genetics RAS, 119333 Moscow, Russia

Phystech School of Biological and Medical Physics, Moscow Institute of Physics and Technology, Dolgoprudny, 141701 Moscow Reg., Russia

Laboratory of Computational Genetics and System Biology, Vavilov Institute of General Genetics RAS, 119333 Moscow, Russia

⁴

Laboratory of Plant Genomics, The Institute for Information Transmission Problems RAS, 127051 Moscow, Russia

⁵

Laboratory of Physiological Plant Pathology, All-Russian Research Institute of Phytopathology, B. Vyazyomy, 143050 Moscow Reg., Russia

⁶

Laboratory of Plant Genetics, Vavilov Institute of General Genetics RAS, 119333 Moscow, Russia

Author to whom correspondence should be addressed.

Pathogens 2019, 8(4), 221; https://doi.org/10.3390/pathogens8040221

Received: 23 September 2019 / Revised: 1 November 2019 / Accepted: 2 November 2019 / Published: 5 November 2019

(This article belongs to the Special Issue Genomics, Molecular, Genetics, and Diversity of Plant Disease Resistance)

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Abstract

Non-specific lipid-transfer proteins (nsLTPs) represent a family of plant antimicrobial peptides (AMPs) implicated in diverse physiological processes. However, their role in induced resistance (IR) triggered by non-pathogenic fungal strains and their metabolites is poorly understood. In this work, using RNA-seq data and our AMP search pipeline, we analyzed the repertoire of nsLTP genes in the wheat Triticum kiharae and studied their expression in response to Fusarium oxysporum infection and treatment with the intracellular metabolites of Fusarium sambucinum FS-94. A total of 243 putative nsLTPs were identified, which were classified into five structural types and characterized. Expression analysis showed that 121 TkLTPs including sets of paralogs with identical mature peptides displayed specific expression patters in response to different treatments pointing to their diverse roles in resistance development. We speculate that upregulated nsLTP genes are involved in protection due to their antimicrobial activity or signaling functions. Furthermore, we discovered that in IR-displaying plants, a vast majority of nsLTP genes were downregulated, suggesting their role as negative regulators of immune mechanisms activated by the FS-94 elicitors. The results obtained add to our knowledge of the role of nsLTPs in IR and provide candidate molecules for genetic engineering of crops to enhance disease resistance. View Full-Text

Keywords: antimicrobial peptides; non-specific lipid-transfer proteins; transcriptome; induced resistance; Triticum kiharae; Fusarium spp.; elicitor metabolites

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MDPI and ACS Style

Odintsova, T.I.; Slezina, M.P.; Istomina, E.A.; Korostyleva, T.V.; Kovtun, A.S.; Kasianov, A.S.; Shcherbakova, L.A.; Kudryavtsev, A.M. Non-Specific Lipid Transfer Proteins in Triticum kiharae Dorof. et Migush.: Identification, Characterization and Expression Profiling in Response to Pathogens and Resistance Inducers. Pathogens 2019, 8, 221. https://doi.org/10.3390/pathogens8040221

AMA Style

Odintsova TI, Slezina MP, Istomina EA, Korostyleva TV, Kovtun AS, Kasianov AS, Shcherbakova LA, Kudryavtsev AM. Non-Specific Lipid Transfer Proteins in Triticum kiharae Dorof. et Migush.: Identification, Characterization and Expression Profiling in Response to Pathogens and Resistance Inducers. Pathogens. 2019; 8(4):221. https://doi.org/10.3390/pathogens8040221

Chicago/Turabian Style

Odintsova, Tatyana I., Marina P. Slezina, Ekaterina A. Istomina, Tatyana V. Korostyleva, Alexey S. Kovtun, Artem S. Kasianov, Larisa A. Shcherbakova, and Alexander M. Kudryavtsev. 2019. "Non-Specific Lipid Transfer Proteins in Triticum kiharae Dorof. et Migush.: Identification, Characterization and Expression Profiling in Response to Pathogens and Resistance Inducers" Pathogens 8, no. 4: 221. https://doi.org/10.3390/pathogens8040221

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Non-Specific Lipid Transfer Proteins in Triticum kiharae Dorof. et Migush.: Identification, Characterization and Expression Profiling in Response to Pathogens and Resistance Inducers

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