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Occurrence of Campylobacter jejuni and Campylobacter coli in Cattle and Sheep in Northern Spain and Changes in Antimicrobial Resistance in Two Studies 10-years Apart

NEIKER—Instituto Vasco de Investigación y Desarrollo Agrario, Animal Health Department, Bizkaia Science and Technology Park 812L, 48160 Derio, Bizkaia, Spain
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Pathogens 2019, 8(3), 98; https://doi.org/10.3390/pathogens8030098
Received: 10 June 2019 / Revised: 1 July 2019 / Accepted: 4 July 2019 / Published: 8 July 2019
(This article belongs to the Section Waterborne/Foodborne/Airborne Pathogens)
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Abstract

A cross-sectional survey was conducted in 2014–2016 in 301 ruminant herds to estimate C. jejuni and C. coli prevalence, and investigate their susceptibility to antimicrobials. Risk of shedding C. jejuni was higher in cattle than sheep (81.2% vs. 45.2%; ORadj = 5.22, p < 0.001), whereas risk of shedding C. coli was higher in sheep than in cattle (19.1% vs. 11.3%; ORadj = 1.71, p = 0.128). Susceptibility to six antimicrobials was determined by broth microdilution using European Committee for Antimicrobial Susceptibility Testing (EUCAST) epidemiological cut-off values. C. coli exhibited higher resistance (94.1%, 32/34) than C. jejuni (65.1%, 71/109), and resistance was more widespread in isolates from dairy cattle than beef cattle or sheep. Compared to results obtained 10-years earlier (2003–2005) in a similar survey, an increase in fluoroquinolone-resistance was observed in C. jejuni from beef cattle (32.0% to 61.9%; OR = 3.45, p = 0.020), and a decrease in tetracycline-resistance in C. jejuni from dairy cattle (75.0% to 43.2%; OR = 0.25, p = 0.026). Resistance to macrolides remained stable at low rates and restricted to C. coli from dairy cattle, with all macrolide-resistant C. coli showing a pattern of pan-resistance. Presence of the single nucleotide polymorphisms (SNPs) associated to quinolone and macrolide resistance was confirmed in all phenotypically resistant isolates. The increase in fluoroquinolone resistance is worrisome but susceptibility to macrolides is reassuring. View Full-Text
Keywords: Campylobacter jejuni; Campylobacter coli; cattle; sheep; antimicrobial resistance (AMR); Minimum Inhibitory Concentration (MIC); Single Nucleotide Polymorphism (SNP) Campylobacter jejuni; Campylobacter coli; cattle; sheep; antimicrobial resistance (AMR); Minimum Inhibitory Concentration (MIC); Single Nucleotide Polymorphism (SNP)
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Ocejo, M.; Oporto, B.; Hurtado, A. Occurrence of Campylobacter jejuni and Campylobacter coli in Cattle and Sheep in Northern Spain and Changes in Antimicrobial Resistance in Two Studies 10-years Apart. Pathogens 2019, 8, 98.

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