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Open AccessArticle

Antifungal and Antivirulence Activity of Vaginal Lactobacillus Spp. Products against Candida Vaginal Isolates

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Laboratório de Microbiologia Aplicada, Programa de Mestrado em Biologia Parasitária, Universidade Ceuma, São Luís, MA 65075120, Brazil
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Programa de Mestrado em Biologia Microbiana, Universidade Ceuma, São Luís, MA 65075120, Brazil
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Laboratório de Biologia Molecular de Microrganismos, Programa de Mestrado em Biologia Microbiana, Universidade Ceuma, São Luís, MA 65075120, Brazil
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Laboratório de Microbiologia Aplicada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270901, Brazil
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Centro de Ciências Biológicas e da Saúde, Universidade Federal do Maranhão, São Luís, MA 65080-805, Brazil
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Programa de Mestrado em Meio Ambiente, Universidade Ceuma, São Luís, MA 65075120, Brazil
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Departamento de Biologia, Instituto Federal do Maranhão, São Luís, MA 65030005, Brazil
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(3), 150; https://doi.org/10.3390/pathogens8030150
Received: 19 August 2019 / Revised: 5 September 2019 / Accepted: 7 September 2019 / Published: 12 September 2019
(This article belongs to the Section Human Pathogens)
Candida yeasts are generally found in the vaginal microbiota; however, disruption of the balance maintained by host factors and microorganisms results in vulvovaginal candidiasis (VVC). This study evaluated the antagonistic activity of vaginal Lactobacillus spp. on Candida albicans to verify whether active compounds of Lactobacillus spp. had antifungal and antivirulence activity. The antagonism assay showed that 15 out of 20 Lactobacillus strains had an inhibitory effect on C. albicans. Biosurfactants displayed surface-tension-reducing activity, with the best value obtained for Lactobacillus gasseri 1. Lactobacillus rhamnosus ATCC 9595, Lactobacillus acidophilus ATCC 4356, and Lactobacillus paracasei 11 produced biosurfactants that decreased C. albicans adhesion and disrupted biofilm formation. The best results were obtained in the pre-incubation assay for L. gasseri 1 and L. paracasei 11. Overall, Lactobacillus strains showed significant anti-Candida activity, and their biosurfactants exhibited considerable anti-adhesion and antibiofilm activity against C. albicans. To be considered safe for use in vivo, the safety of biosurfactant (BS) should be investigated using cytotoxicity assays. View Full-Text
Keywords: Lactobacillus biosurfactant production; biofilm inhibition; Candida albicans suppression; Lactobacillus antivirulence; vulvovaginal candidiasis Lactobacillus biosurfactant production; biofilm inhibition; Candida albicans suppression; Lactobacillus antivirulence; vulvovaginal candidiasis
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Itapary dos Santos, C.; Ramos França, Y.; Duarte Lima Campos, C.; Quaresma Bomfim, M.R.; Oliveira Melo, B.; Assunção Holanda, R.; Santos, V.L.; Gomes Monteiro, S.; Buozzi Moffa, E.; Souza Monteiro, A.; Andrade Monteiro, C.; Monteiro-Neto, V. Antifungal and Antivirulence Activity of Vaginal Lactobacillus Spp. Products against Candida Vaginal Isolates. Pathogens 2019, 8, 150.

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