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In Vitro Evaluation of Combined Commercialized Ophthalmic Solutions Against Acanthamoeba Strains

Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, España
Departamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, Cd. Obregón, Sonora 95370, Mexico
Departmento de Oftalmología, Hospital Universitario de Canarias, 38320 La Laguna, Tenerife, Canary Islands, Spain
Laboratoire Matériaux-Molécules et Applications, La Marsa, University of Carthage, Tunisia 1003, Tunisia
Author to whom correspondence should be addressed.
Pathogens 2019, 8(3), 109;
Received: 11 July 2019 / Revised: 22 July 2019 / Accepted: 24 July 2019 / Published: 25 July 2019
(This article belongs to the Special Issue Emerging Parasitic Protozoa)
Acanthamoeba is a free-living amoebae genus which is present worldwide in natural and artificial environments. These amoebae are clinically important as causative agents of diseases in humans and other animals such as a fatal encephalitis or a sight threatening Acanthamoeba keratitis (AK). Lately; studies have focused on the search of novel therapeutic options for AK but also to prevent infections. Furthermore; the evaluation of commercialized products seems to be an option for this case since not clinical assays would be required. Thus; we aimed to test the amoebicidal activity of different mixtures of two commercial ophthalmic solutions: Systane® Ultra; which has already shown anti-Acanthamoeba properties; and Naviblef® Daily Care. In addition, we tested their cytotoxic effect against murine macrophages. At the individual level; Naviblef® Daily Care showed to be the most active product against Acanthamoeba spp. Nevertheless; the combinations of Systane® Ultra and Naviblef® Daily Care; showed an improvement in the activity against trophozoites and cysts of Acanthamoeba castellanii Neff. Moreover; the concentration necessary to generate cytotoxic effect against murine macrophages (J774.1) was much higher than the required for the amoebicidal and cysticidal effect achieved in the most effective mixtures. View Full-Text
Keywords: Acanthamoeba; keratitis; ophthalmic solution; chemotherapy; amoebicidal activity Acanthamoeba; keratitis; ophthalmic solution; chemotherapy; amoebicidal activity
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MDPI and ACS Style

Reyes-Batlle, M.; Mura-Escorche, G.; Sifaoui, I.; Otero-Ruiz, A.; Alfaro-Sifuentes, R.; López-Arencibia, A.; Rocha-Cabrera, P.; Chiboub, O.; Rizo-Liendo, A.; Zamora-Herrera, J.; Bethencourt-Estrella, C.J.; Rodríguez-Expósito, R.L.; San Nicolás-Hernández, D.; Piñero, J.E.; Lorenzo-Morales, J. In Vitro Evaluation of Combined Commercialized Ophthalmic Solutions Against Acanthamoeba Strains. Pathogens 2019, 8, 109.

AMA Style

Reyes-Batlle M, Mura-Escorche G, Sifaoui I, Otero-Ruiz A, Alfaro-Sifuentes R, López-Arencibia A, Rocha-Cabrera P, Chiboub O, Rizo-Liendo A, Zamora-Herrera J, Bethencourt-Estrella CJ, Rodríguez-Expósito RL, San Nicolás-Hernández D, Piñero JE, Lorenzo-Morales J. In Vitro Evaluation of Combined Commercialized Ophthalmic Solutions Against Acanthamoeba Strains. Pathogens. 2019; 8(3):109.

Chicago/Turabian Style

Reyes-Batlle, María, Glorian Mura-Escorche, Ines Sifaoui, Alejandro Otero-Ruiz, Rosalía Alfaro-Sifuentes, Atteneri López-Arencibia, Pedro Rocha-Cabrera, Olfa Chiboub, Aitor Rizo-Liendo, Jonadab Zamora-Herrera, Carlos J. Bethencourt-Estrella, Rubén L. Rodríguez-Expósito, Desirée San Nicolás-Hernández, José E. Piñero, and Jacob Lorenzo-Morales. 2019. "In Vitro Evaluation of Combined Commercialized Ophthalmic Solutions Against Acanthamoeba Strains" Pathogens 8, no. 3: 109.

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