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Pathogens 2019, 8(1), 7;

Molecular Characterisation of Equine Herpesvirus 1 Isolates from Cases of Abortion, Respiratory and Neurological Disease in Ireland between 1990 and 2017

Virology Unit, The Irish Equine Centre, Johnstown, Naas, Co. Kildare W91 RH93, Ireland
Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh EH8 9XD, UK
Department of Mathematics and Statistics, University of Limerick, Limerick V94 T9PX, Ireland
Department of Biological Sciences, University of Limerick, Limerick V94 T9PX, Ireland
Author to whom correspondence should be addressed.
Received: 17 December 2018 / Revised: 4 January 2019 / Accepted: 8 January 2019 / Published: 15 January 2019
(This article belongs to the Section Animal Pathogens)
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Multiple locus typing based on sequencing heterologous regions in 26 open reading frames (ORFs) of equine herpesvirus 1 (EHV-1) strains Ab4 and V592 was used to characterise 272 EHV-1 isolates from 238 outbreaks of abortion, respiratory or neurological disease over a 28-year period. The analysis grouped the 272 viruses into at least 10 of the 13 unique long region (UL) clades previously recognised. Viruses from the same outbreak had identical multi-locus profiles. Sequencing of the ORF68 region of EHV-1 isolates from 222 outbreaks established a divergence into seven groups and network analysis demonstrated that Irish genotypes were not geographically restricted but clustered with viruses from all over the world. Multi-locus analysis proved a more comprehensive method of strain typing than ORF68 sequencing. It was demonstrated that when interpreted in combination with epidemiological data, this type of analysis has a potential role in tracking virus between premises and therefore in the implementation of targeted control measures. Viruses from 31 of 238 outbreaks analysed had the proposed ORF30 G2254/D752 neuropathogenic marker. There was a statistically significant association between viruses of the G2254/D752 genotype and both neurological disease and hypervirulence as defined by outbreaks involving multiple abortion or neurological cases. The association of neurological disease in those with the G2254/D752 genotype was estimated as 27 times greater than in those with the A2254/N752 genotype. View Full-Text
Keywords: EHV-1; multi-locus typing; ORF68; neuropathogenic marker EHV-1; multi-locus typing; ORF68; neuropathogenic marker

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Garvey, M.; Lyons, R.; Hector, R.D.; Walsh, C.; Arkins, S.; Cullinane, A. Molecular Characterisation of Equine Herpesvirus 1 Isolates from Cases of Abortion, Respiratory and Neurological Disease in Ireland between 1990 and 2017. Pathogens 2019, 8, 7.

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