Next Article in Journal
Non-typhoidal Salmonella in the Pig Production Chain: A Comprehensive Analysis of Its Impact on Human Health
Previous Article in Journal
Virus–Host Interactions Involved in Lassa Virus Entry and Genome Replication
Open AccessArticle

Potato Pathogens in Russia’s Regions: An Instrumental Survey with the Use of Real-Time PCR/RT-PCR in Matrix Format

1
Russian Agricultural Center, Moscow 107139, Russia
2
GenBit LLC, Nauchny pr. 20/2, Moscow 117246, Russia
3
All-Russian Research Institute of Phytopathology, Bolshie Vyazemy 143050, Russia
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(1), 18; https://doi.org/10.3390/pathogens8010018
Received: 27 November 2018 / Revised: 26 January 2019 / Accepted: 26 January 2019 / Published: 29 January 2019
(This article belongs to the Section Plant Pathogens)
Viral and bacterial diseases of potato cause significant yield loss worldwide. The current data on the occurrence of these diseases in Russia do not provide comprehensive understanding of the phytosanitary situation. Diagnostic systems based on disposable stationary open qPCR micromatrices intended for the detection of eight viral and seven bacterial/oomycetal potato diseases have been used for wide-scale screening of target pathogens to estimate their occurrence in 11 regions of Russia and to assess suitability of the technology for high-throughput diagnostics under conditions of field laboratories. Analysis of 1025 leaf and 725 tuber samples confirmed the earlier reported data on the dominance of potato viruses Y, S, and M in most regions of European Russia, as well as relatively high incidences of Clavibacter michiganensis subsp. sepedonicus, Pectobacterium atrosepticum, and P. carotovorum subsp. carotovorum, and provided detailed information on the phytosanitary status of selected regions and geographical spread of individual pathogens. Information on the occurrence of mixed infections, including their composition, was the first data set of this kind for Russia. The study is the first large-scale screening of a wide range of potato pathogens conducted in network mode using unified methodology and standardized qPCR micromatrices. The data represent valuable information for plant pathologists and potato producers and indicate the high potential of the combined use of matrix PCR technology and network approaches to data collection and analysis with the view to rapidly and accurately assess the prevalence of certain pathogens, as well as the phytosanitary state of large territories. View Full-Text
Keywords: real-time PCR; potato viral diseases; potato bacterial diseases; high-throughput diagnostics; matrix approach; multiplex pathogen identification; occurrence of pathogens real-time PCR; potato viral diseases; potato bacterial diseases; high-throughput diagnostics; matrix approach; multiplex pathogen identification; occurrence of pathogens
Show Figures

Figure 1

MDPI and ACS Style

Malko, A.; Frantsuzov, P.; Nikitin, M.; Statsyuk, N.; Dzhavakhiya, V.; Golikov, A. Potato Pathogens in Russia’s Regions: An Instrumental Survey with the Use of Real-Time PCR/RT-PCR in Matrix Format. Pathogens 2019, 8, 18.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop