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Pathogens 2018, 7(4), 79; https://doi.org/10.3390/pathogens7040079

Effect of Cetylpyridinium Chloride (CPC) on Colony Formation of Common Nontuberculous Mycobacteria

Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061, USA
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Received: 18 September 2018 / Revised: 25 September 2018 / Accepted: 30 September 2018 / Published: 5 October 2018
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Abstract

Cetylpyridinium chloride (CPC) is widely used to decontaminate water samples for the cultivation of nontuberculous mycobacteria (NTM). The rationale for using CPC is that it kills more non mycobacteria than NTM and thereby prevents the outgrowth and detection of mycobacterial colonies on solid media. The few CPC-susceptibility measurements that have been published, suggest that CPC-decontamination does kill significant numbers of NTM. We confirm that observation here and further demonstrate that CPC-susceptibility varied significantly by one log between representative NTM species and between strains of the same species. CPC-susceptibility was the same for cells collected from cultures or water-acclimated (P = 0.6485, T-test) and CPC-susceptibility was relatively similar over the range of commonly employed CPC dosages. We conclude that use of CPC as decontaminating agent may lead to failure to recover an NTM isolate and considerable underestimates of NTM numbers. View Full-Text
Keywords: nontuberculous mycobacteria; cetylpyridinium chloride; decontamination nontuberculous mycobacteria; cetylpyridinium chloride; decontamination
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Williams, M.D.; Falkinham, J.O., III. Effect of Cetylpyridinium Chloride (CPC) on Colony Formation of Common Nontuberculous Mycobacteria. Pathogens 2018, 7, 79.

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