Effect of Cetylpyridinium Chloride (CPC) on Colony Formation of Common Nontuberculous Mycobacteria
Abstract
:1. Introduction
2. Results
2.1. Effect of 0.1% CPC Exposure for 30 Minutes on CFU/mL
2.2. Effect of Different CPC Dosages on CFU/mL
3. Discussion
4. Materials and Methods
4.1. Mycobacterium spp. Strains
4.2. Growth and Collection of Mycobacterium spp. Strains
4.3. Water Acclimation
4.4. Measurement of the Effect of CPC on Colony-Forming Units (CFU) /mL
4.5. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Strain | Percent Survival a (T-test) bCulture-Grown | Percent Survival a (T-test) bWater-Acclimated |
---|---|---|
M. avium A5 | 21.7% (0.0049) | 20.6% (0.0125) |
M. avium Va14 (T) | 64.7% (0.3176) | 81.3% (0.0748) |
M. avium Va14 (O) | 1.6% (0.0088) | 3.8% (0.0017) |
M. intracellulare 1406T | 13.4% (0.0427) | 23.1% (0.0009) |
M. chimaera MA3323 | 21.2% (0.0029) | 26.3% (0.0061) |
M. chimaera NC-W-2.1 | 64.0% (0.0158) | 68.9% (0.0132) |
M. abscessus AAy-P-1 | 34.2% (0.0076) | 24.0% (0.0014) |
M. abscessus PS-W-4-1 | 62.6% (0.1057) | 23.9% (0.0014) |
M. abscessus MHS-Sw-7-1 | 33.5% (0.0008) | 6.6% (< 0.0001) |
M. abscessus 12-9-Sw-B-2 | 5.9% (< 0.0001) | 6.4% (0.0041) |
M. abscessus 12-45-Sw-A-1 | 25.0% (0.0041) | 36.7% (0.0242) |
CPC (% wt/vol) | Exposure (min) | Per Cent Survival aCulture-Grown |
---|---|---|
0 (Control) | 0 (Control) | 100% |
0.05% | 30 min | 16.7 ± 2.8 |
0.10% | 15 min | 15.4 ± 2.7 |
0.10% | 30 min | 16.4 ± 5.0 |
0.10% | 60 min | 12.2 ± 3.7 |
0.20% | 30 min | 9.0 ± 0.1 |
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Williams, M.D.; Falkinham, J.O., III. Effect of Cetylpyridinium Chloride (CPC) on Colony Formation of Common Nontuberculous Mycobacteria. Pathogens 2018, 7, 79. https://doi.org/10.3390/pathogens7040079
Williams MD, Falkinham JO III. Effect of Cetylpyridinium Chloride (CPC) on Colony Formation of Common Nontuberculous Mycobacteria. Pathogens. 2018; 7(4):79. https://doi.org/10.3390/pathogens7040079
Chicago/Turabian StyleWilliams, Myra D., and Joseph O. Falkinham, III. 2018. "Effect of Cetylpyridinium Chloride (CPC) on Colony Formation of Common Nontuberculous Mycobacteria" Pathogens 7, no. 4: 79. https://doi.org/10.3390/pathogens7040079