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Pathogens 2018, 7(2), 47; https://doi.org/10.3390/pathogens7020047

Different Dose-Dependent Modes of Action of C-Type Natriuretic Peptide on Pseudomonas aeruginosa Biofilm Formation

1
Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Université, University Rouen-Normandy, 27000 Evreux, France
2
Laboratoire de Biotechnologie et Chimie Marines (LBCM), EA 3884, LBCM, IUEM Université de Bretagne-Sud, 56100 Lorient, France
3
Inserm U1239, PRIMACEN, Normandie Université, IRIB, Université de Rouen, 76000 Rouen, France
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 1 February 2018 / Revised: 16 April 2018 / Accepted: 20 April 2018 / Published: 24 April 2018
(This article belongs to the Special Issue Signaling Systems in Pseudomonas aeruginosa Biofilm)
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Abstract

We have previously shown that the C-type Natriuretic Peptide (CNP), a peptide produced by lungs, is able to impact Pseudomonas aeruginosa physiology. In the present work, the effect of CNP at different concentrations on P. aeruginosa biofilm formation was studied and the mechanisms of action of this human hormone on P. aeruginosa were deciphered. CNP was shown to inhibit dynamic biofilm formation in a dose-dependent manner without affecting the bacterial growth at any tested concentrations. The most effective concentrations were 1 and 0.1 µM. At 0.1 µM, the biofilm formation inhibition was fully dependent on the CNP sensor protein AmiC, whereas it was only partially AmiC-dependent at 1 µM, revealing the existence of a second AmiC-independent mode of action of CNP on P. aeruginosa. At 1 µM, CNP reduced both P. aeruginosa adhesion on glass and di-rhamnolipid production and also increased the bacterial membrane fluidity. The various effects of CNP at 1 µM and 0.1 µM on P. aeruginosa shown here should have major consequences to design drugs for biofilm treatment or prevention. View Full-Text
Keywords: biofilm; hormones; peptides; bacterial adaptation; lung; cystic fibrosis biofilm; hormones; peptides; bacterial adaptation; lung; cystic fibrosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Desriac, F.; Clamens, T.; Rosay, T.; Rodrigues, S.; Tahrioui, A.; Enault, J.; Roquigny, L.; Racine, P.-J.; Taupin, L.; Bazire, A.; Dufour, A.; Leprince, J.; Bouffartigues, E.; Chevalier, S.; Feuilloley, M.G.J.; Lesouhaitier, O. Different Dose-Dependent Modes of Action of C-Type Natriuretic Peptide on Pseudomonas aeruginosa Biofilm Formation. Pathogens 2018, 7, 47.

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