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Article

Staphylococcus aureus is More Prevalent in Retail Beef Livers than in Pork and other Beef Cuts

by
Lubna S. Abdalrahman
,
Harrington Wells
and
Mohamed K. Fakhr
*
Department of Biological Science, The University of Tulsa, Tulsa, OK 74104, USA
*
Author to whom correspondence should be addressed.
Pathogens 2015, 4(2), 182-198; https://doi.org/10.3390/pathogens4020182
Submission received: 11 August 2014 / Revised: 20 April 2015 / Accepted: 22 April 2015 / Published: 28 April 2015
(This article belongs to the Special Issue Foodborne Pathogens)
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Abstract

:
Staphylococcus aureus is one of the top five pathogens contributing to acquired foodborne illnesses causing an estimated quarter million cases every year in the US. The objectives of this study were to determine the prevalence of Methicillin Susceptible S. aureus (MSSA) and Methicillin Resistant S. aureus (MRSA) in retail beef livers, beef, and pork meats sold in Tulsa, Oklahoma and to characterize the recovered strains for their virulence and antimicrobial resistance. Ninety six chilled retail beef (50 beef livers and 46 beef other cuts), and 99 pork meat samples were collected. The prevalence in beef livers was 40/50 (80%) followed by other beef cuts 23/46 (50%) then pork 43/99 (43.3%). No isolates were positive for MRSA since none harbored the mecA or mecC gene. A total of 334 recovered S. aureus isolates (143 beef livers, 76 beef, and 115 pork isolates) were screened for their antimicrobial susceptibility against 16 different antimicrobials and their possession of 18 different toxin genes. Multidrug resistance was more prevalent in the pork isolates followed by beef then beef livers. The prevalence of enterotoxin genes such as seg, seh, and sei and the toxic shock syndrome gene tst was higher in the pork isolates than in the beef ones. The hemolysin genes, particularly hlb, were more prevalent in isolates from beef livers. Molecular typing of a subset of the recovered isolates showed that they are highly diverse where spa typing was more discriminatory than PFGE. The alarmingly high incidence of S. aureus in retail beef livers in this study should raise awareness about the food safety of such meat products.

1. Introduction

Foodstuff contamination with Staphylococcus aureus such as in dairy products and retail meats may occur as a result of poor hygiene during handling the food or directly from infected food-producing animals [1]. Symptoms of Staphylococcus poisoning which include diarrhea, abdominal cramps, vomiting, and nausea occur within 24 h after consuming toxin-contaminated food [2]. Most clinical signs of staphylococcal food poisoning are self-limited and disappear within 24–48 h after ingestion of the toxins. Death is rare and usually occurs in infants or the elderly [3]. More recent reviews are available discussing S. aureus in food and livestock [4,5,6].
Staphylococcal food poisoning has been considered an important cause of food-borne diseases around the world [7]. Some studies reported that Staphylococcal food poisoning has been the third most common cause of food-borne diseases in the last few decades worldwide [8]. Food-borne outbreaks in Europe between 1993 and 1998 were about 5.1% S. aureus [9]. Staphylococcus aureus is listed among the top five pathogens contributing to domestically acquired foodborne illnesses causing as many as 241,148 cases annually in the United States [10]. Both Staphylococcus aureus and MRSA have been isolated from chicken, pork, beef and others. For instance, one study in Japan found two isolates of MRSA out of 444 samples of raw chicken meats at 145 different supermarkets [11]. Other surveys in the Netherlands and Canada reported 20–40% of samples from pigs were positive for MRSA [12,13]. New different clones of MRSA that were related to pigs and cattle farming were detected in the Netherlands [14,15]. Recently, few studies reported variable prevalence of S. aureus and/or MRSA in retail meats at different US locations including Louisiana [16], Maryland, USA [17], Detroit, Michigan [18], Iowa [19], Minnesota, and New Jersey [20], Georgia [21], and North Dakota [22,23].
S. aureus is a bacterium of major concern worldwide due to its resistance to different types of antibiotics including beta-lactam antimicrobials [24]. A study from Louisiana [25] found that most S. aureus in different types of meat were commonly resistant to penicillin (71%), ampicillin (68%), tetracycline (67%), erythromycin (30%), clindamycin (18%), oxacillin with up to 2% of sodium chloride (14%), levofloxacin (13%), ciprofloxacin (13%), gentamicin (3%), dapfopristtin (3%), chloramphenical (2%), and moxifloxacin (1%). Another research study in Italy found that most S. aureus strains isolated from different foods were resistant to several types of antibiotics [1].
S. aureus is considered as one of the most dangerous pathogenic bacteria due to the production of a variety of extracellular protein toxins such as toxic shock syndrome toxin 1 (TSST-1), exfoliative toxin (ET), coagulase, hemolysins, and at least 15 types of enterotoxins [26]. Toxic shock syndrome of S. aureus causes staphylococcal scarlet fever. The production of exfoliative toxin of this bacterium causes staphylococcal scalded-skin syndrome, also known as Ritter’s disease [27]. Toxic shock syndrome toxin-1 (TSST-1) and enterotoxins (ETA, ETB) are the main ones responsible for toxin syndromes and food-borne diseases in humans and animals. Both of them belong to a family of superantigens [28]. Most S. aureus isolates produce different types of enterotoxins that are considered the main cause of food poisoning [27]. Many research studies have detected the prevalence of different types of toxin genes that are produced by S. aureus in different types of meats. For example, one of the studies by Pu et al. (2011) concluded that the high percentage of staphylococcal enterotoxins from Louisiana retail meat was 66% for seg and sei, 20% for seh, 15% for sed, 13% for sej, and 1% for sea [25]. Another research study in Korea reported different types of toxin genes in raw meats, which included toxic shock syndrome-1 (TSST-1) and enterotoxins [29].
Only a limited number of studies investigating the prevalence of MRSA in the food chain in the US were conducted in the last few years [17]. The number of studies discussing the prevalence of S. aureus in beef livers is very limited in the literature [30]. The objectives of this study were to determine the prevalence of Staphylococcus aureus and MRSA in retail beef livers, beef, and pork meats sold in the Tulsa, Oklahoma area and to characterize the recovered strains for their virulence and antimicrobial resistance.

2. Results and Discussion

2.1. Prevalence of Staphylococcus aureus and MRSA in Beef Livers, Beef and Pork

A total of 195 chilled retail beef and pork meat samples were purchased from several Tulsa area grocery stores. The samples were purchased weekly from January to June of 2010. There were 96 beef samples and 99 pork samples used in this study (Table 1). Fifty of the beef samples were beef livers and 46 were from other beef cuts like steak, shoulder, stew, neck, and bone in, etc. (Table 1). As shown in Table 1, the overall prevalence of S. aureus in beef was 63/96 (65.6%), while 43/99 (43.3%) of pork samples were contaminated with S. aureus. Also the prevalence of Staphylococcus aureus in beef livers 40/50 (80%) was higher than for other beef cuts (50%) (Table 1). So, the prevalence of S. aureus was higher in beef livers (80%) followed by beef (50%) then pork (43%). No isolates of beef livers, beef, or pork samples were positive for MRSA since none of them carried mecA or mecC genes.
Table
Table 1. Prevalence of Staphylococcus aureus in the beef livers, beef, and pork samples.
Table 1. Prevalence of Staphylococcus aureus in the beef livers, beef, and pork samples.
Prevalence of Staphylococcus aureus
BeefPork np/n (%)
Beef Liver *np/n (%)Beef (Other Cuts) np/n (%)Total np/n (%)
40/50 (80%)23/46 (50%)63/96 (65.6%)43/99 (43.3%)
* np: Number of positive samples; n: Number of samples collected.
Statistical comparison of beef livers, beef cuts and pork showed that significant differences exist among the three groups in incidence of S. aureus (X2 = 18.36, df = 2, p < 0.001). Thus, we went on to make pairwise comparisons. Beef cuts and pork where not significantly different (X2 = 0.55, df = 1, p > 0.25), and so these two groups were combined for comparison to beef livers. Beef livers when compared to {beef cuts + pork} were significant different in the incidence of S. aureus (X2 = 17.82, df = 2, p < 0.001). Beef livers harbor S. aureus more often than cuts of beef or pork.
Also, the overall prevalence of S. aureus in beef in this study was (65.6%) (Table 1), which is significantly higher than a study that showed 20% (comparison of our data to a model with 20% incidence: X2 = 124.90, df = 1, p < 0.001) [16], and one that showed 37% (comparison of our data to a model with 37% incidence: X2 = 33.75, df = 1, p < 0.001) [31] of beef samples were contaminated with S. aureus. Another research study in the Netherlands reported that the prevalence of S. aureus in beef was 33.3% [32], which is also significantly different from our finding. We clearly see that our study represents the highest prevalence of S. aureus in beef, but we attribute this to the fact that we included samples from beef livers that showed very high prevalence of 80%. The percentage of S. aureus in pork in our study was 43.3% (Table 1), which is almost in agreement with other published studies [31,32]. In Louisiana, a study found 45.6% of pork samples contained S. aureus, as did 20% of beef samples, while MRSA was found in 5.6% of pork samples and 3.3% of beef samples [16]. S. aureus was isolated from 56% of ground turkey, 28% of ground beef and only 12% of ground pork samples collected from Maryland, USA [17]. S. aureus was found in 20.5% of retail beef in Detroit, Michigan where two isolates were MRSA [18]. In another study in Iowa, 18.2% of retail pork and 6.9% of retail beef was positive for S. aureus where two of the pork isolates were identified as MRSA [19]. S. aureus was isolated from 64.8% of retail pork products in Iowa, Minnesota, and New Jersey in 2012 where 6.6% of the isolated strains were MRSA [20]. In a more recent study in Georgia, USA, S. aureus was isolates from 45% of retail pork and 63% of beef products where 3% of beef and 4% of pork were contaminated with MRSA [21]. Another study in North Dakota, USA showed a S. aureus prevalence of 49.3% in retail pork where 7% were MRSA [22]. A later study showed the prevalence of S. aureus in retail beef in Fargo, ND to be 9/36 and in retail pork to be 25/37 with slightly higher detection when realtime PCR was used [23]. An older study showed the prevalence of staphylococci to be 42% in beef liver and 27% in pork chops [30]. There are many factors that make the comparisons between different studies difficult and also these factors may contribute to the variety of positive percentages. Factors like different processing facilities, sampling, geographic locations, brand, isolation methods, and collection time can explain the variable prevalence of S. aureus among different studies in the literature.

2.2. Antimicrobial Resistance Screening

A total of 334 S. aureus recovered isolates (219 beef isolates [143 beef livers and 76 other beef cuts] and 115 pork isolates) were subjected to antimicrobial resistance profiling against 16 different antimicrobials that belong to ten different antibiotic classes (Table 4). As shown in Table 2 the percentage of resistance of the pork isolates was higher than beef isolates for all the antibiotics tested. The percentage of resistance of other beef cuts isolates was also higher than in beef liver isolates for almost all the antibiotics tested except rifampin and vancomycin. Resistance to oxacillin and cefoxitin was higher in the pork isolates (>40%) than in the beef ones despite the fact that no isolates in this study contained the mecA or mecC gene, so no genotypic MRSA was detected.
The distribution of Multidrug Resistance (MDR) among the 219 S. aureus beef isolates (143 beef livers and 76 other beef cuts) was as follows: 128 beef liver isolates and 43 other beef cuts isolates were resistant to one to four antimicrobials, 13 beef liver isolates and 16 other beef cuts isolates were resistant to five to seven antimicrobials, and two beef liver isolates and 17 other beef cuts isolates were resistant to more than seven antimicrobials (Figure 1). Also, the distribution of Multidrug Resistance (MDR) among the 115 S. aureus pork isolates was as follows: 12 isolates resistant to one to four antimicrobials, 31 isolates resistant to five to seven antimicrobials, and 72 isolates resistant to more than seven antimicrobials (Figure 1). Most of the pork isolates were highly multidrug resistant being resistant to more than seven antimicrobials. Also, most beef livers isolates were resistant to one to four antimicrobials indicating a lower level of multidrug resistance than the beef and pork ones.
Table
Table 2. Antimicrobial resistance of the Staphylococcus aureus beef livers, beef, and pork isolates against 16 different antimicrobials. A test of the three S. aureus populations (beef livers, beef cuts, pork) for homogeneity in distribution of positive isolates across populations was performed for each antibiotic. Results: ** for p < 0.01, * for p < 0.05, and ns for not significant.
Table 2. Antimicrobial resistance of the Staphylococcus aureus beef livers, beef, and pork isolates against 16 different antimicrobials. A test of the three S. aureus populations (beef livers, beef cuts, pork) for homogeneity in distribution of positive isolates across populations was performed for each antibiotic. Results: ** for p < 0.01, * for p < 0.05, and ns for not significant.
Antimicrobial Resistance
AntibioticBeefPork np/n (%)
Beef Livers * np/n (%)Beef (Other Cuts) np/n (%)Total np/n (%)
azithromycin **5/143 (3.5)31/76 (40.8)36/219 (16.4)75/115 (65.2)
ciprofloxacin **1/143 (0.7)15/76 (19.7)16/219 (7.3)47/115 (40.9)
gentamicin **8/143 (5.6)22/76 (28.9)30/219 (13.7)68/115 (59.1)
oxacillin **10/143 (6.9)6/76 (7.9)16/219 (7.3)56/115 (48.7)
cefoxitin **11/143 (7.7)8/76 (10.5)19/219 (8.7)47/115 (40.9)
tetracycline **21/143 (14.7)33/76 (43.4)54/219 (24.7)109/115 (94.8)
vancomycin **12/143 (8.4)6/76 (7.9)18/219 (8.2)49/115 (42.6)
doxycycline **10/143 (6.9)26/76 (34.2)36/219 (16.4)96/115 (83.5)
trimethoprirm/ sulfamethazole **1/143 (0.7)2/76 (2.6)3/219 (1.4)22/115 (19.1)
clindamycin **1/143 (0.7)9/76 (11.8)10/219 (4.5)25/115 (21.7)
penicillin **31/143 (21.7)48/76 (63.2)79/219 (36.1)102/115 (88.7)
ampicillin ns142/143 (99.3)76/76 (100)218/219 (99.5)115/115 (100)
kanamycin **4/143 (2.8)23/76 (30.3)27/219 (12.3)62/115 (53.9)
erythromycin **8/143 (5.6)31/76 (40.8)39/219 (17.8)63/115 (54.8)
rifampin *21/143 (14.7)6/76 (7.9)27/219 (12.3)25/115 (21.7)
chloramphenicol **3/143 (2.1)6/76 (7.9)9/219 (4.1)35/115 (30.4)
np: Number of positive isolates; n: Number of isolates collected.
A test of the three S. aureus populations (beef livers, beef cuts, pork) for homogeneity in distribution of MDR (three categories: 1–4, 5–7, 8+) showed that there were significant difference (X2 = 173.16, df = 4, p < 0.001). Thus, we went on to make pairwise comparisons. All three pairwise comparisons were significant (X2 ≥ 37.41, df = 2, p < 0.001 in each case). Beef livers showed the lowest and pork the greatest MDR levels.
In this study, beef isolates were highly resistant to ampicillin (36.1%) followed by tetracycline (24.7%) (Table 2). High resistance in pork isolates in our study were as follows: Ampicillin (100%), tetracycline (94.8%), penicillin (88.7%), doxycycline (83.5%); however, in a previous study from Louisiana, antimicrobial resistance of beef and pork samples was slightly lower: Penicillin (71%), ampicillin (68%) and tetracycline (67%) [16]. In a different study, 70% and 73% of S. aureus isolated strains were resistant to ampicillin and penicillin, respectively [33]. In another study, more turkey and pork isolates were resistant to ampicillin, penicillin and tetracycline than the beef ones [17]. Resistance to vancomycin was also significantly higher in pork isolates compared to beef isolates (Table 2). In a recent study, the authors indicated that high levels of in-feed zinc and other disinfectants used in commercial swine herds might be possible drivers in the selection and persistence of MRSA in the herds [33]. They also suggested that these agents may be co-selecting for other antimicrobial resistance genes [33]. It is reasonable to propose that the higher vancomycin resistance reported in pork compared to beef in our study might be resulting from the heavy use of antimicrobial feed additives, heavy metals and disinfectants in swine production. In the present study, MDR was common among S. aureus isolates. Isolates that were resistant to more than seven antimicrobials were prevalent in the pork isolates. Another study reported that isolates resistant to three or more antimicrobials were found in turkey, followed by those from pork, and chicken [31].
In our study, there was a significant number of S. aureus strains (>40% in the pork isolates) that showed the same phenotypic behavior as MRSA by showing resistance to both cefoxitin and oxacillin (Table 2). Genotypically, they were not MRSA, since they did not possess the mecA or the mecC gene. Phenotypic MRSA isolates that do not contain the mecA gene were also detected previously [17]. In a study in Portugal, 38% of S. aureus isolates from various foods were resistant to oxacillin but only 0.68% showed the presence of mecA gene [34]. This behavior can be due to the overproduction of Beta-lactamase enzymes or might be due to the presence of a MRSA variant mecA gene that cannot be detected with the currently available PCR primers. Two recent studies exploring the presence of such variant mecA genes support this hypothesis [35,36].
Pathogens 04 00182 g001 1024
Figure 1. Distribution of the total number of Staphylococcus aureus isolates isolated from beef livers, beef, and pork according to their Multidrug Resistance (MDR) to one to four antimicrobials, five to seven antimicrobials, and more than seven antimicrobials of the 16 antimicrobials tested.
Figure 1. Distribution of the total number of Staphylococcus aureus isolates isolated from beef livers, beef, and pork according to their Multidrug Resistance (MDR) to one to four antimicrobials, five to seven antimicrobials, and more than seven antimicrobials of the 16 antimicrobials tested.
Pathogens 04 00182 g001

2.3. Toxin Gene Possession Screening

A total of 334 Staphylococcus aureus isolates (219 beef isolates (143 beef livers and 76 other beef cuts) and 115 pork isolates) were screened for 18 different toxin genes that belong to six different toxin gene groups (Table 3). As shown in Table 3, S. aureus hemolysin genes were found at a higher percentage in beef and pork than other groups of toxin genes. Also, no isolates of beef and pork harbored enterotoxin genes seb-sec or see, the exfloliative toxin genes eta or etb, or the Leucocidin gene lukM. Also, the prevalence of hemolysin genes hla (93.2%) and hld (93.2%) in beef isolates were higher than in the pork ones, where hla was 86.9% and hld was 86.9%. Hemolysin gene hlb was present more often in beef than in pork. No isolates out of 219 of S. aureus from beef was positive for the entoretoxin gene sec, while one isolate (0.9%) from pork was positive for this gene. Also, two isolates out of 219 of S. aureus from beef were positive for the entoretoxin genes sed (0.9%) and sej (0.9%), while no isolates from pork was positive for these two genes. The percentage of toxic shock syndrome toxin 1 gene tst from pork isolates (13%) was higher than beef isolates (1.8%). One isolate out of 115 of S. aureus from pork was positive for the PVL gene lukS-lukF (0.9%), while no isolates from beef was positive for this particular gene. As shown in Table 3, the percentage of the lukE-lukD gene in other beef cuts isolates was higher than from beef liver isolates. Also, the percentage of the hlb gene in beef liver isolates was higher than from other beef cuts isolates.
Table
Table 3. Toxin gene screening of the Staphylococcus aureus beef livers, beef, and pork isolates to 18 different toxin genes. A test of the three S. aureus populations (beef livers, beef cuts, pork) for homogeneity in distribution of positive isolates across populations was performed for each toxin gene. Since many expected cells are small Yates correction was used throughout. Results: *** for p < 0.001, ** for p < 0.01, * for p < 0.05, and ns for not significant.
Table 3. Toxin gene screening of the Staphylococcus aureus beef livers, beef, and pork isolates to 18 different toxin genes. A test of the three S. aureus populations (beef livers, beef cuts, pork) for homogeneity in distribution of positive isolates across populations was performed for each toxin gene. Since many expected cells are small Yates correction was used throughout. Results: *** for p < 0.001, ** for p < 0.01, * for p < 0.05, and ns for not significant.
Prevalence of Toxin Genes
Toxin GeneBeefPork np/n (%)
Beef Livers * np/n (%)Beef ( Other Cuts) np/n (%)Total np/n (%)
sea ns0/143 (0.0)2/76 (2.6)2/219 (0.9)0/115 (0)
seb-sec ns0/143 (0.0)0/76 (0.0)0/219 (0)0/115 (0)
sec ns0/143 (0.0)0/76 (0.0)0/219 (0)1/115 (0.9)
sed ns0/143 (0.0)2/76 (2.6)2/219 (0.9)0/115 (0)
see ns0/143 (0.0)0/76 (0.0)0/219 (0)0/115 (0)
seg **4/143 (2.8)5/76 (6.6)9/219 (4.1)18/115 (15.7)
seh ns0/143 (0.0)0/76 (0.0)0/219 (0)36/115 (31.3)
sei **2/143 (1.4)11/76 (14.5)13/219 (5.9)8/115 (6.9)
sej ns1/143 (0.7)1/76 (1.32)2/219 (0.9)0/115 (0)
tst ***4/143 (2.8)0/76 (0.0)4/219 (1.8)15/115 (13.0)
eta ns0/143 (0.0)0/76 (0.0)0/219 (0)0/115 (0)
etb ns0/143 (0.0)0/76 (0.0)0/219 (0)0/115 (0)
lukE-lukD ***12/143 (8.4)24/76 (31.6)36/219 (16.4)41/115 (35.7)
lukM ns0/143 (0.0)0/76 (0.0)0/219 (0)0/115 (0)
hla ***141/143 (98.6)63/76 (82.9)204/219 (93.2)100/115 (86.9)
hlb ***128/143 (89.5)38/76 (50.0)166/219 (75.8)41/115 (35.7)
hld ***141/143 (98.6)63/76 (82.9)204/219 (93.2)100/115 (86.9)
lukS-lukF ns0/143 (0.0)0/76 (0.0)0/219 (0)1/115 (0.9)
* np: Number of positive isolates; n: Number of isolates collected.
In the Louisiana study, most of their beef and pork isolates were positive for seg, sei (66%), followed by seh (20%), sed (15%), sej (13%), and sea (1%) and no isolates were positive for enterotoxins sec, seb, or see, the toxic shock syndrome toxin 1 gene tst, or the exfloliative toxin genes eta, or etb [16]. The presence of tst in 13% of our pork isolates is alarming. The higher prevalence of hemolysin genes, particularly hlb, in beef livers might be due to the availability of blood in the liver. Other than the Louisiana study mentioned above, the rest of the US studies concerning the prevalence of S. aureus and MRSA in retail meats did not screen their recovered isolates for the possession of several toxin genes. A study in Italy reported that the prevalence of enterotoxin genes for S. aureus was 58.8% in meat and dairy products [1]. Therefore, as was the case in the prevalence and antimicrobial resistance data, toxin gene possession can also vary by meat type, processing facility, location, and brand. It is interesting to report that the prevalence of toxin genes in retail meat samples in this study appears generally lower than those previously reported in human Staphylococcus aureus (both MSSA and MRSA) with the exception of hemolysin genes where the prevalence was only slightly lower than in the human strains [37].
The high prevalence of S. aureus in retail meats in our study, particularly in beef livers (80%), is of concern, particularly that a recent study proved the potential of MRSA transfer from retail pork onto food contact surfaces and the possibility of consumer exposure [38]. Another study demonstrated that MRSA acquired on pig farms can be transferred through processing in the slaughter house [39]. Colonization of pork butchers with livestock associated MRSA (LA-MRSA) that was suggested to be acquired by cross-contamination from pork was recently proposed [40].
The high prevalence of S. aureus in retail beef livers is alarming and might be due to cross contamination since livers are recovered from several cows in slaughter houses and possibly piled up together. In Campylobacter, Ghafir et al. [41] suggested that the high level of recovery of the bacterium from livers is probably due to the fact that the liver surface stays moist, which might protect this foodborne pathogen. A possible explanation can be also true in S. aureus. Human handlers are possible sources of contaminating beef livers in slaughter houses. The risk of the high prevalence of S. aureus in beef liver in our study could be more severe due to the fact that livers are usually lightly cooked to avoid the undesired taste of overcooking. Adding to this risk is the fact that some S. aureus enterotoxins are heat stable. We have recently also found high prevalence of Campylobacter in beef livers [42] and chicken livers [43].
The high prevalence of S. aureus strains on retail beef livers, beef, and pork sold in Oklahoma that belong to major brands is alarming. While the absence of MRSA in our collected retail meats is considered good news, a good percentage of the isolated non-MRSA Staphylococcus aureus strains were highly resistant to multiple antimicrobials and possess several toxin genes. More prevalence studies should be conducted in the US to gather more data that can help in reducing the presence of this important foodborne pathogen in retail meats.

2.4. Molecular Typing Using Spa Typing and PFGE

A subset of S. aureus recovered strains (three from beef livers, two from beef other cuts, and six from pork) and representing different brands and cuts were subjected to molecular typing by spa typing (Figure 2) and Pulsed Field Gel Electrophoresis (PFGE) (Figure 3). As it is shown in Figure 2, there was no direct correlation between a specific spa type and a particular meat source except that four of the pork isolates were clustered together. Strains isolated from each of the meat sources showed high level of diversity in regards to their spa types (Figure 2). Even though four strains were non-typable by PFGE, PFGE was able to separate the strains according to their meat source into two major clusters (Figure 3). As it is shown in Figure 3, pork isolates were clustered together in one cluster separate from those isolated from beef. It is worthy to note that strains of the same PFGE pattern showed different spa types (Figure 3). So, spa typing appears to be more discriminatory than PFGE in this regard which was previously reported [37]. It is also clear that S. aureus strains isolated from beef and pork in this study were highly diverse using spa typing (Figure 2). Nasal carriage and clinical MSSA isolates were previously reported to be more variable than CA-MRSA [37].
Pathogens 04 00182 g002 1024
Figure 2. A dendrogram showing spa typing for a subset of the recovered Staphylococcus aureus strains representing different meat sources, cuts, and brands. Strains isolated from the same meat source are labeled by the same color square.
Figure 2. A dendrogram showing spa typing for a subset of the recovered Staphylococcus aureus strains representing different meat sources, cuts, and brands. Strains isolated from the same meat source are labeled by the same color square.
Pathogens 04 00182 g002
Pathogens 04 00182 g003 1024
Figure 3. Pulsed Field Gel Electrophoresis (PFGE) patterns of a subset of the recovered Staphylococcus aureus strains representing different meat sources and brands showing their corresponding spa types. Four strains were not typable by PFGE and hence they are not shown in the dendrogram.
Figure 3. Pulsed Field Gel Electrophoresis (PFGE) patterns of a subset of the recovered Staphylococcus aureus strains representing different meat sources and brands showing their corresponding spa types. Four strains were not typable by PFGE and hence they are not shown in the dendrogram.
Pathogens 04 00182 g003

3. Experimental Section

3.1. Isolation of Staphylococcus aureus from Retail Meat Samples

Meat samples were collected from several different grocery stores in the Tulsa, Oklahoma area on weekly bases from January to June 2010. A total of 195 chilled retail beef and pork meat samples were used in this study (96 beef samples [50 beef livers and 46 beef other cuts] and 99 pork samples) (Table 1). Meat samples were purchased from nine grocery stores that belong to six different franchises chains at variable locations in the city. The collected beef samples belonged to nine different brands while the pork ones were from seven brands. Pork and beef samples other than livers were from different cuts such as, steak, stew, shoulder, neck, and bone etc. Samples were selected to be variable as possible with different expiration dates and production codes. Meat samples were rinsed with 10 mL of buffered peptone water (BPW) (BPW; EMD, Gibbstown, NJ, USA) in sterile plastic bags (VWR Scientific, Radnor, PA, USA) and then massaged by hand for 5 min. Then, 10 mL the rinse was enriched in 10 mL of enrichment broth of 2 X Trypticase Soy Broth with 10% sodium chloride and 1% sodium pyruvate. The enrichment was incubated at 37 °C for 24 h and then streaked to Baird Parker (BP) selective media plates. Later, all plates were incubated at 37 °C for 48 h [16]. Four suspected S. aureus colonies (those that have black colonies surrounded by 2–5 mm clear zones) were selected and streaked to Trypticase Soy Agar (TSA) plates and subcultured for confirmation on MSA (Mannitol Salt Agar) plates.

3.2. DNA Extraction

The single cell lysing buffer (SCLB) method was used to extract bacterial DNA for polymerase chain reaction (PCR) from prospective S. aureus cultures as described previously [44].

3.3. PCR Identification

A multiplex PCR reaction was used to identify the isolated suspected S. aureus by using specific primers for S. aureus and MRSA to amplify a 108 bp [45] and a 312 bp [12] fragments respectively. The multiplex PCR was performed as described previously [16]. Isolates showing resistance to cefoxitin and/or oxacillin were subjected to PCR confirmation using a second set of MRSA primers that amplify a 533 bp mecA fragment [46] and two other variant MRSA mecA primer sets (also known as mecC) that amplify 356 bp [35] and 1800 bp [36] fragments to confirm the MRSA phenotype.

3.5. Antimicrobial Resistance Screening

A total number of 334 Staphylococcus aureus isolates (219 beef isolates (143 beef livers and 76 other beef cuts) and 115 pork isolates) were subjected to antimicrobial susceptibility testing against 16 different antimicrobials that belong to ten different antibiotic classes (Table 4). Isolates were grown on Mueller-Hinton (MH) agar (Difco) and incubated for 48 h at 37 °C. Cultures were then added to Mueller-Hinton broth (Difco), adjusted to turbidity equal to a 0.5 McFarland standard, and inoculated onto 6-inch MH agar plates supplemented with the appropriate antimicrobial at different concentrations (Table 4) including the breakpoint established for each antimicrobial according to the Clinical and Laboratory Standards Institute (CLSI) when available [47]. The plates were incubated at 37 °C for 48 h. The plates were read for growth or no growth and denoted as resistant or susceptible, respectively, according to the breakpoints for each of the 16 tested antimicrobials (Table 4).
Table
Table 4. A list of the 16 tested antimicrobials, their classes, the concentrations used for susceptibility testing, and the breakpoints used for each antimicrobial.
Table 4. A list of the 16 tested antimicrobials, their classes, the concentrations used for susceptibility testing, and the breakpoints used for each antimicrobial.
Antimicrobial ClassAntimicrobialsConc. 1 (µg/mL)Conc. 2 (µg/mL) (Break point)Conc. 3 (µg/mL)Conc. 4 (µg/mL)
β-Lactamspenicillin0.1250.250.51
ampicillin0.250.512
oxacillin + 2% Nacl24816
cefoxitin + 2% Nacl481632
Tetracyclinestetracycline8163264
doxycycline8163264
Macrolidesazithromycin481632
erythromycin481632
Aminoglycosideskanamycin3264128256
gentamicin8163264
Fluoroquinolonesciprofloxacin24816
Lincosamidesclindamycin24816
Phenicolschloramphenicol163264128
Glycopeptidesvancomycin163264128
Rifamycinesrifampin24816
Sulfonamidestrimethoprim/sulfamethoxazole2/384/768/15216/304

3.6. Prevalence of Toxin Genes

A total of 334 Staphylococcus aureus isolates (219 beef isolates (143 beef livers and 76 other beef cuts) and 115 pork isolates) were screened for 18 different toxin genes that belong to six different toxin gene groups. Multiplex PCR was used to detect 18 different toxin genes of S. aureus isolates that include enterotoxins (sea, seb-sec, sec, sed, see, seg, seh, sei, sej), toxic shock syndrome toxin 1 (tst), exfoliative toxins (eta, atb), leucocidins (lukE-lukD, lukM), Panton-Valentine leucocidin (PVL) (lukS-lukF), and hemolysins (hla, hlb, hld). Three multiplex reactions (A, B, and C), each of which included six toxin genes, were performed (Table 5). The multiplex PCRs targeting the toxin genes were performed in a 20 μL reaction solution that contained 10 μL of Green Master Mix (Promega), 2 μL of sterile water, 2 μL of the DNA template and 0.5 μL of each of the toxin gene primers. The thermocycling protocol for toxin genes included an initial denaturation at 95 °C for 5 min, followed by 30 cycles of denaturation (94 °C for 1 min), annealing (57 °C for 1 min), and extension (72 °C for 1 min), ending with an extension at 72 °C for 7 min. Then 10 μL of the multiplex PCR product was added to a 2% (wt/vol) agarose gel in 1x Tris-acetate-EDTA (TAE) buffer. A 100–10,000 bp ladder was used as a molecular marker. The electrophoresis was run at 100 mV for 1 h and 10 min. The gel was stained for 15 min using ethidium bromide and distained in distilled water for 30 min. The DNA bands were visualized and recorded under UV using a gel documentation system. The expected amplicon band sizes of S. aureus toxin genes are shown in Table 5. At least one PCR amplicon of each positively reported toxin gene was sequenced using the same amplifying primers to ensure the accuracy of the PCR amplification.
Table
Table 5. Multiplex PCR primers, reaction sets, and references for toxin genes screening.
Table 5. Multiplex PCR primers, reaction sets, and references for toxin genes screening.
Toxin GeneSize (bp)Primer sequences (5'-3')Multiplex PCR Reaction SetRef.
sea521GCA GGG AAC AGC TTT AGG CA[48]
GTT CTG TAG AAG TAT GAA ACA CG
seb-sec665ATG TAA TTT TGA TAT TCG CAG TGA[48]
TGC AGG CAT CAT ATC ATA CCA
sec284CTT GTA TGT ATG GAG GAA TAA CAAA[48]
TGC AGG CAT CAT ATC ATA CCA
sed385GTG GTG AAA TAG ATA GGA CTG CA[48]
ATA TGA AGG TGC TCT GTG G
see171TAC CAA TTA ACT TGT GGA TAG ACA[48]
CTC TTT GCA CCT TAC CGC
seg328CGT CTC CAC CTG TTG AAG GA[48]
CCA AGT GAT TGT CTA TTG TCG
seh359CAA CTG CTG ATT TAG CTC AGB[48]
GTC GAA TGA GTA ATC TCT AGG
sei466CAA CTC GAA TTT TCA ACA GGT ACB[48]
CAG GCA GTC CAT CTC CTG
sej142CAT CAG AAC TGT TGT TCC GCT AGB[48]
CTG AAT TTT ACC ATC AAA GGT AC
tst560GCT TGC GAC AAC TGC TAC AGB[48]
TGG ATC CGT CAT TCA TTG TTA A
eta93GCA GGT GTT GAT TTA GCA TTB[26]
AGA TGT CCC TAT TTT TGC TG
etb226ACA AGC AAA AGA ATA CAG CGB[26]
GTT TTT GGC TGC TTC TCT TG
lukS-lukF433ATC ATT AGG TAA AAT GTC TGG ACA TGA TCC AC[27]
GCA TCA AST GTA TTG GAT AGC AAA AGC
lukE-lukD269TGA AAA AGG TTC AAA GTT GAT ACG AGC[27]
TGT ATT CGA TAG CAA AAG CAG TGC A
lukM780TGG ATG TTA CCT ATG CAA CCT ACC[27]
GTT CGT TTC CAT ATA ATG AAT CAC TAC
hla209CTG ATT ACT ATC CAA GAA ATT CGA TTGC[27]
CTT TCC AGC CTA CTT TTT TAT CAG T
hlb309GTG CAC TTA CTG ACA ATA GTG CC[27]
GTT GAT GAG TAG CTA CCT TCA GT
hld111AAG AAT TTT TAT CTT AAT TAA GGA AGG AGT GC[27]
TTA GTG AAT TTG TTC ACT GTG TCG A

3.7. Molecular Typing of Staphylococcus aureus Isolates

A subset of the recovered S. aureus isolates were subjected to molecular typing using spa typing and Pulsed Field Gel Electrophoresis (PFGE). Isolates were chosen to represent a percentage of the positive samples of each meat source (beef, beef livers, and pork). Within each meat source, isolates were chosen to be as variable as possible to represent different brands, meat cuts, dates of collection, antimicrobial susceptibility and toxin profiles. The molecular typing using spa was done according to published primers and protocols [49] and spa types were assigned using the BioNumerics Softwatre (Applied Math, Austin, TX, USA). PFGE was performed according to the CDC protocol [50]. The digested plugs were run in Seakem agarose gel (Lonza, Allendale, NJ, USA) with 0.5× Tris-Borate EDTA (TBE) buffer (Amresco, Solon, OH, USA) to separate the bands on the CHEF Mapper PFGE system (Bio-Rad). Gel images were analyzed using BioNumerics software (Applied Maths, Austin, TX, USA). The banding patterns were clustered using Dice coefficients and unweighted pair group method, with arithmetic mean (UPGMA), and a 3% band tolerance.

4. Conclusions

The prevalence of Staphylococcus aureus in retail beef livers in this study was alarmingly high. The prevalence of this foodborne pathogen in retail beef and pork, while lower than beef livers, is still very significant. Multidrug resistance was generally higher in the pork isolates followed by the beef and beef liver ones. While no isolate harbored the mecA gene, a good percentage of the pork isolates were phenotypically similar to MRSA strains by being resistant to cefoxitin and oxacillin. Few of the S. aureus strains recovered from retail meats in this study possessed several toxin genes including enterotoxins. Molecular typing of a subset of the recovered isolates showed that they are highly diverse where spa typing was more discriminatory than PFGE. Even though the prevalence of enterotoxin genes in beef livers was lower than beef and pork meats, care should be taken not to leave beef livers for longer periods of time at room temperature prior to cooking to reduce the chance of thermostable enterotoxin production, particularly as livers are usually not overcooked.

Acknowledgments

The authors would like to acknowledge financial support from The University of Tulsa Office of Research and Sponsored Programs (The Student Research Grant Program) in subsidizing the purchase of the retail meat samples used in this study. The authors are also grateful to Peggy Hill for the English language editing of the manuscript. Lubna S. Abdalrahman is grateful to the Libyan Government for financial support during her M.S. study in the U.S.

Author Contributions

M.K.F. suggested and designed the experiments. L.A. performed the experiments. H.W. performed and wrote the statistical analysis part of the manuscript. L.A. and M.K.F. wrote and revised the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Normanno, G.; Firinu, A.; Virgilio, S.; Mula, G.; Dambrosio, A.; Poggiu, A.; Decastelli, L.; Mioni, R.; Scuota, S.; Bolzoni, G.; et al. Coagulase-positive Staphylococci and Staphylococcus aureus in food products marketed in Italy. Int. J. Food Microbiol. 2005, 98, 73–79. [Google Scholar] [PubMed]
  2. Lee, J.H. Methicillin (oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl. Environ. Microbiol. 2003, 69, 6489–6494. [Google Scholar] [CrossRef] [PubMed]
  3. Martin, S.E.; Iandolo, J.J. Staphylococcus. In Encyclopedia of Food Microbiology; Robinson, R.K., Batt, C.A., Patel, P.D., Eds.; Academic press: New York, NY, USA, 2000; pp. 2062–2065. [Google Scholar]
  4. Doyle, M.E.; Hartmann, F.A.; Lee Wong, A.C. Methicillin-resistant staphylococci: Implications for our food supply? Anim. Health Res. Rev. 2012, 13, 157–180. [Google Scholar] [CrossRef] [PubMed]
  5. Verkade, E.; Kluytmans, J. Livestock-associated Staphylococcus aureus CC398: Animal reservoirs and human infections. Infect. Genet. Evol. 2014, 21, 523–530. [Google Scholar] [CrossRef] [PubMed]
  6. Kadariya, J.; Smith, T.C.; Thapaliya, D. Staphylococcus aureus and staphylococcal food-borne disease: An ongoing challenge in public health. BioMed. Res. Int. 2014, 2014, 827965. [Google Scholar] [CrossRef] [PubMed]
  7. Genigeorgis, C.A. Present state of knowledge on staphylococcal intoxication. Int. J. Food Microbiol. 1989, 9, 327–360. [Google Scholar] [CrossRef] [PubMed]
  8. Zhang, S.; Iandolo, J.J.; Stewart, G.C. The enterotoxin D plasmid of Staphylococcus aureus encodes a second enterotoxin determinant (sej). FEMS Microbial. Lett. 1998, 168, 227–233. [Google Scholar] [CrossRef]
  9. Tirado, C.; Schimdt, K. WHO surveillance programme for control of foodborne infections and intoxications: Preliminary results and trends across greater Europe. J. Infect. 2001, 43, 80–84. [Google Scholar] [CrossRef] [PubMed]
  10. Centers for Disease Control (CDC). CDC Estimates of Foodborne Illness in the United States. 2011. Available online: http://www.cdc.gov/foodborneburden/2011-foodborne-estimates.html (accessed on 16 May 2013). [Google Scholar]
  11. Kitai, S.; Shimizu, A.; Kawano, J.; Sato, E.; Nakano, C.; Uji, T.; Kitagawa, H. Characterization of methicillin-resistant Staphylococcus aureus isolated from retail raw chicken meat in Japan. J. Vet. Med. Sci. 2005, 67, 107–110. [Google Scholar] [CrossRef] [PubMed]
  12. De Neeling, A.J.; van Leeuwen, W.J.; Schouls, L.M.; Schot, C.S.; van Veen-Rutgers, A.; Beunders, A.J.; Buiting, A.G.; Hol, C.; Ligtvoet, E.E.; Petit, P.L.; et al. Resistance of staphylococci in The Netherlands: Surveillance by an electronic network during 1989–1995. J. Antimicrob. Chemoth. 1998, 41, 93–101. [Google Scholar] [CrossRef]
  13. Khanna, T.; Friendship, R.; Dewey, C.; Weese, J.S. Methicillin resistant Staphylococcus aureus colonization in pigs and pig farmers. Vet. Microbiol. 2008, 128, 298–303. [Google Scholar] [CrossRef] [PubMed]
  14. Voss, A.; Loeffen, F.; Bakker, J.; Klaassen, C.; Wulf, M. Methicillin resistant Staphylococcus aureus in pig farming. Emerg. Infect. Dis. 2005, 11, 1965–1966. [Google Scholar] [CrossRef] [PubMed]
  15. Van den Eede, A.; Hermans, K.; Lipinska, U.; Struelens, M.; Deplano, A.; Denis, O.; Gasthuys, F.; Haesebrouck, F.; Martens, A. Nasal carriage of methicillin-resistance Staphylococcus aureus in the equine population: Prevalence, typing and antimicrobial resistance. In Proceedings of the 2nd Symposium on Antimicrobial Resistance in Animals and the Environment, Tours, France, 17–19 December 2007.
  16. Pu, S.; Wang, F.; Ge, B. Isolation and characterization of methicillin-resistant Staphylococcus aureus strains from Louisiana retail meats. Appl. Environ. Microb. 2009, 75, 265–267. [Google Scholar] [CrossRef]
  17. Kelman, A.; Soong, Y.A.; Dupuy, N.; Shafer, D.; Richbourg, W.; Johnson, K.; Meng, J. Antimicrobial susceptibility of Staphylococcus aureus from retail ground meats. J. Food Prot. 2011, 74, 1625–1629. [Google Scholar] [CrossRef] [PubMed]
  18. Bhargava, K.; Wang, X.; Donabedian, S.; Zervos, M.; da Rocha, L.; Zhang, Y. Methicillin-resistant Staphylococcus aureus in retail meat, Detroit, Michigan, USA. Emerg. Infect. Dis. 2011, 17, 1135. [Google Scholar] [CrossRef] [PubMed]
  19. Hanson, B.M.; Dressler, A.E.; Harper, A.L.; Scheibel, R.P.; Wardyn, S.E.; Roberts, L.K.; Smith, T.C. Prevalence of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) on retail meat in Iowa. J. Infect. Public Health 2011, 4, 169–174. [Google Scholar] [CrossRef] [PubMed]
  20. O’Brien, A.M.; Hanson, B.M.; Farina, S.A.; Wu, J.Y.; Simmering, J.E.; Wardyn, S.E.; Smith, T.C. MRSA in conventional and alternative retail pork products. PLoS ONE 2012, 7, e30092. [Google Scholar] [CrossRef] [PubMed]
  21. Jackson, C.R.; Davis, J.A.; Barrett, J.B. Prevalence and characterization of methicillin-resistant Staphylococcus aureus isolates from retail meat and humans in Georgia. J. Clin. Microbiol. 2013, 51, 1199–1207. [Google Scholar] [CrossRef] [PubMed]
  22. Buyukcangaz, E.; Velasco, V.; Sherwood, J.S.; Stepan, R.M.; Koslofsky, R.J.; Logue, C.M. Molecular typing of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) isolated from animals and retail meat in North Dakota, United States. Foodborne Pathog. Dis. 2013, 10, 608–617. [Google Scholar] [CrossRef] [PubMed]
  23. Velasco, V.; Sherwood, J.S.; Rojas-García, P.P.; Logue, C.M. Multiplex real-time PCR for detection of Staphylococcus aureus, mecA and Panton-Valentine Leukocidin (PVL) genes from selective enrichments from animals and retail meat. PloS ONE 2014, 9, e97617. [Google Scholar] [CrossRef] [PubMed]
  24. David, M.Z.; Daum, R.S. Community-associated methicillin-resistant Staphylococcus aureus: Epidemiology and clinical consequences of an emerging epidemic. Clin. Microbiol. Rev. 2010, 23, 616–687. [Google Scholar] [CrossRef] [PubMed]
  25. Pu, S.; Wang, F.; Ge, B. Characterization of toxin genes and antimicrobial susceptibility of Staphylococcus aureus isolates from Louisiana retail meats. Foodborne Pathog. Dis. 2011, 8, 299–306. [Google Scholar] [CrossRef] [PubMed]
  26. Mehrotra, M.; Wang, G.; Johnson, W.M. Multiplex PCR for detection of genes for Staphylococcu aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J. Clin. Microbiol. 2000, 38, 1032–1035. [Google Scholar] [PubMed]
  27. Jarraud, S.; Mougel, C.; Thioulouse, J.; Lina, G.; Meugnier, H.; Forey, F.; Nesme, X.; Etienne, J.; Vandenesch, F. Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infect. Immun. 2002, 70, 631–641. [Google Scholar] [CrossRef] [PubMed]
  28. Gunaydin, B.; Aslantas, O.; Demir, C. Detection of superantigenic toxin genes in Staphylococcus aureus strains from subclinical bovine mastitis. Trop. Anim. Health Prod. 2011, 43, 1633–1637. [Google Scholar] [PubMed]
  29. Hwang, S.Y.; Kim, S.H.; Jang, E.J.; Kwon, N.H.; Park, Y.K.; Koo, H.C.; Jung, W.K.; Kim, J.M.; Park, W.H. Novel multiplex PCR for the detection of the Staphylococcus aureus superantigen and its application to raw meat isolates in Korea. Int. J. Food Microbiol. 2007, 117, 99–105. [Google Scholar] [CrossRef] [PubMed]
  30. Jay, J.M. Further studies on staphylococci in meats, III. Occurrence and characteristics of coagulase-positive strains from a variety of nonfrozen market cuts. Appl. Microbiol. 1962, 10, 247–251. [Google Scholar] [PubMed]
  31. Waters, A.E.; Contente-Cuomo, T.; Buchhagen, J.; Liu, C.M.; Watson, L.; Pearce, K.; Foster, J.T.; Bowers, J.; Driebe, E.M.; Engelthaler, D.M.; et al. Multidrug-resistant Staphylococcus aureus in US meat and poultry. Clin. Infect. Dis. 2011, 52, 1–4. [Google Scholar] [CrossRef] [PubMed]
  32. Van Loo, I.H.; Diederen, B.M.; Savelkoul, P.H.; Woudenberg, J.H.; Roosendaal, R.; van Belkum, A.; Lemmens-den Toom, N.; Verhulst, C.; van Keulen, P.H.; Kluytmans, J.A. Methicillin-resistant Staphylococcus aureus in meat products, the Netherlands. Emerg. Infect. Dis. 2007, 13, 1753–1755. [Google Scholar] [CrossRef] [PubMed]
  33. HSlifierz, M.J.; Friendship, R.M.; Weese, J.S. Methicillin-resistant Staphylococcus aureus in commercial swine herds is associated with disinfectant and zinc usage. Appl. Environ. Microbiol. 2015, 81, 2690–2695. [Google Scholar] [CrossRef] [PubMed]
  34. Pereira, V.; Lopes, C.; Castro, A.; Silva, J.; Gibbs, P.; Teixeira, P. Characterization for enterotoxin production, virulence factors, and antibiotic susceptibility of Staphylococcus aureus isolates from various foods in Portugal. Food Microbiol. 2009, 26, 278–282. [Google Scholar] [CrossRef] [PubMed]
  35. Alvarez, L.G.; Holden, M.T.G.; Lindsay, H.; Webb, C.R.; Brown, D.F.J.; Curran, M.D.; Walpole, E.; Brooks, K.; Pickard, D.J.; Teale, C.; et al. Methicillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: A descriptive study. Lancet 2011, 11, 595–603. [Google Scholar] [CrossRef] [PubMed]
  36. Shore, A.C.; Deasy, E.C.; Slickers, P.; Brennan, G.; Connell, B.O.; Monecke, S.; Ehricht, R.; Coleman, D.C. Detection of staphylococcal cassette chromosome mec Type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Ch. 2011, 55, 3765–3773. [Google Scholar] [CrossRef]
  37. Snyder, H.L.; Niebuhr, S.E.; Dickson, J.S. Transfer of Methicillin-Resistant Staphylococcus aureus from Retail Pork Products onto Food Contact Surfaces and the Potential for Consumer Exposure. J. Food Protect. 2013, 76, 2087–2092. [Google Scholar] [CrossRef]
  38. Shukla, S.K.; Karow, M.E.; Brady, J.M.; Stemper, M.E.; Kislow, J.; Moore, N.; Wroblewski, K.; Chyou, P.-H.; Warshauer, D.M.; Reed, K.D.; et al. Virulence genes and genotypic associations in nasal carriage, community-associated methicillin-susceptible and methicillin-resistant USA400 Staphylococcus aureus isolates. J. Clin. Microbiol. 2010, 48, 3582–3592. [Google Scholar] [CrossRef] [PubMed]
  39. Hawken, P.; Weese, J.S.; Friendship, R.; Warriner, K. Longitudinal study of clostridium difficile and methicillin-resistant Staphylococcus aureus associated with pigs from weaning through to the end of processing. J. Food Protect. 2013, 76, 624–630. [Google Scholar] [CrossRef]
  40. Boost, M.; Ho, J.; Guardabassi, L.; O’Donoghue, M. Colonization of butchers with livestock-associated methicillin-resistant Staphylococcus aureus. Zoonoses Public Health 2013, 60, 572–576. [Google Scholar] [CrossRef] [PubMed]
  41. Ghafir, Y.; China, B.; Dierick, K.; de Zutter, L.; Daube, G. A seven-year survey of Campylobacter contamination in meat at different production stages in Belgium. Int. J. Food Microbiol. 2007, 116, 111–120. [Google Scholar] [CrossRef] [PubMed]
  42. Noormohamed, A.; Fakhr, M.K. A higher prevalence rate of Campylobacter in retail beef livers compared to other beef and pork meat cuts. Int. J. Environ. Res. Public Health 2013, 10, 2058–2068. [Google Scholar] [CrossRef] [PubMed]
  43. Noormohamed, A.; Fakhr, M.K. Incidence and antimicrobial resistance profiling of Campylobacter in retail chicken livers and gizzards. Foodborne Pathog. Dis. 2012, 9, 617–624. [Google Scholar] [CrossRef] [PubMed]
  44. Marmur, J. A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Bio. 1961, 3, 208–218. [Google Scholar]
  45. Martineau, F.; Picard, F.J.; Roy, P.H.; Ouellette, M.; Bergeron, M.G. Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus. J. Clin. Microbiol. 1998, 36, 618–623. [Google Scholar] [PubMed]
  46. Murakami, K.; Minamide, W.; Wada, K.; Nakamura, E.; Teraoka, H.; Watanabe, S. Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. J. Clin. Microbiol. 1991, 29, 2240–2244. [Google Scholar]
  47. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 2011; 31, 1–172. [Google Scholar]
  48. Monday, S.R.; Bohach, G.A. Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in staphylococcal isolates. J. Clin. Microbiol. 1999, 37, 3411–3414. [Google Scholar]
  49. Shopsin, B.; Gomez, M.; Montgomery, S.O.; Smith, D.H.; Waddington, M.; Dodge, D.E.; Bost, D.E.; Riehman, M.; Naidich, S.; Kreiswirth, B.N. Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains. J. Clin. Microbiol. 1999, 37, 3556–3563. [Google Scholar] [PubMed]
  50. McDougal, L.K.; Steward, C.D.; Killgore, G.E.; Chaitram, J.M.; McAllister, S.K.; Tenover, F.C. Pulsed-field gel electrophoresis typing of oxacillin-resistant Staphylococcus aureus isolates from the United States: Establishing a national database. J. Clin. Microbiol. 2003, 41, 5113–5120. [Google Scholar] [CrossRef] [PubMed]

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MDPI and ACS Style

Abdalrahman, L.S.; Wells, H.; Fakhr, M.K. Staphylococcus aureus is More Prevalent in Retail Beef Livers than in Pork and other Beef Cuts. Pathogens 2015, 4, 182-198. https://doi.org/10.3390/pathogens4020182

AMA Style

Abdalrahman LS, Wells H, Fakhr MK. Staphylococcus aureus is More Prevalent in Retail Beef Livers than in Pork and other Beef Cuts. Pathogens. 2015; 4(2):182-198. https://doi.org/10.3390/pathogens4020182

Chicago/Turabian Style

Abdalrahman, Lubna S., Harrington Wells, and Mohamed K. Fakhr. 2015. "Staphylococcus aureus is More Prevalent in Retail Beef Livers than in Pork and other Beef Cuts" Pathogens 4, no. 2: 182-198. https://doi.org/10.3390/pathogens4020182

APA Style

Abdalrahman, L. S., Wells, H., & Fakhr, M. K. (2015). Staphylococcus aureus is More Prevalent in Retail Beef Livers than in Pork and other Beef Cuts. Pathogens, 4(2), 182-198. https://doi.org/10.3390/pathogens4020182

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