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Antibiotic-Free Selection in Biotherapeutics: Now and Forever

Technology Research Institute Bioaster, 317 avenue Jean-Jaurés, 69007 Lyon, France
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Academic Editor: Lawrence Young
Pathogens 2015, 4(2), 157-181; https://doi.org/10.3390/pathogens4020157
Received: 9 January 2015 / Revised: 9 March 2015 / Accepted: 23 March 2015 / Published: 3 April 2015
The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations. Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles. The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means. This short review will present examples of alternatives to antibiotic selection and their context of application to highlight their ineluctable invasion of the bio-therapeutic world. View Full-Text
Keywords: antibiotic-free selection; auxotrophy; post-seggregational killing; minicircle antibiotic-free selection; auxotrophy; post-seggregational killing; minicircle
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Mignon, C.; Sodoyer, R.; Werle, B. Antibiotic-Free Selection in Biotherapeutics: Now and Forever. Pathogens 2015, 4, 157-181.

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