Next Article in Journal
Staphylococcus aureus is More Prevalent in Retail Beef Livers than in Pork and other Beef Cuts
Previous Article in Journal
Phytogenic Compounds as Alternatives to In-Feed Antibiotics: Potentials and Challenges in Application
Article Menu

Export Article

Open AccessReview

Antibiotic-Free Selection in Biotherapeutics: Now and Forever

Technology Research Institute Bioaster, 317 avenue Jean-Jaurés, 69007 Lyon, France
Author to whom correspondence should be addressed.
Academic Editor: Lawrence Young
Pathogens 2015, 4(2), 157-181;
Received: 9 January 2015 / Revised: 9 March 2015 / Accepted: 23 March 2015 / Published: 3 April 2015
PDF [1013 KB, uploaded 3 April 2015]


The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations. Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles. The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means. This short review will present examples of alternatives to antibiotic selection and their context of application to highlight their ineluctable invasion of the bio-therapeutic world. View Full-Text
Keywords: antibiotic-free selection; auxotrophy; post-seggregational killing; minicircle antibiotic-free selection; auxotrophy; post-seggregational killing; minicircle

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Mignon, C.; Sodoyer, R.; Werle, B. Antibiotic-Free Selection in Biotherapeutics: Now and Forever. Pathogens 2015, 4, 157-181.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Pathogens EISSN 2076-0817 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top