First Detection of West Nile Virus Lineage 2 in Mosquitoes in Switzerland, 2022
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling
2.2. Screening of Mosquito Pools and FTA Cards
2.3. Sequencing and Phylogenetic Analysis
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer | Genome Position 1 | Sequence (5′–3′) 2 | Product Size (bp) |
---|---|---|---|
LSWNV3’F | 10,459–10,478 | CGCCACCGGAAGTTGAGTAG | 111 |
LSWNV3’R | 10,569–10,550 | CTCCTTCCGAGACGGTTCTG | |
LSWNV3’P | 10,483–10,503 | TGCTGCCTGCGDCTCAACCCC | |
LSWNV5’F | 7–27 | TCGCCTGTGTGAGCTGACAAA | 112 |
LSWNV5’R | 118–96 | GCCCTCCTGGTTTCTTAGACATC | |
LSWNV5’P | 89–66 | TTCGTGCYAAGAAACAGCTCGCAC |
Sample ID | Sampling Period | Matrix | Ct Value 1 | Accession No. | % Genome Covered |
---|---|---|---|---|---|
TI_SUPSI_C28 | 2–9 August | FTA card | 28.6 | OR091151 | 92.69 |
TI_SUPSI_C37 | 9–17 August | FTA card | 24.2 | OR091152 | 97.58 |
TI_SUPSI_C65 | 28 August–6 September | FTA card | 27.8 | OR091153 | 95.96 |
TI_SUPSI_C70 | 6–12 September | FTA card | 28.0 | OR091154 | 98.26 |
TI_SUPSI_M63 | 19 July–2 August | Culex pipiens | 31.5 | OR091155 | 95.83 |
TI_SUPSI_M158 | 9–17 August | Culex pipiens | 29.3 | OR091156 | 96.76 |
TI_SUPSI_M224 | 28 August–6 September | Culex pipiens | 27.9 | OR091158 | 98.23 |
TI_SUPSI_M202 | 27 September–5 October | Culex pipiens | 14 | OR091157 | 99.19 |
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Cazzin, S.; Liechti, N.; Jandrasits, D.; Flacio, E.; Beuret, C.; Engler, O.; Guidi, V. First Detection of West Nile Virus Lineage 2 in Mosquitoes in Switzerland, 2022. Pathogens 2023, 12, 1424. https://doi.org/10.3390/pathogens12121424
Cazzin S, Liechti N, Jandrasits D, Flacio E, Beuret C, Engler O, Guidi V. First Detection of West Nile Virus Lineage 2 in Mosquitoes in Switzerland, 2022. Pathogens. 2023; 12(12):1424. https://doi.org/10.3390/pathogens12121424
Chicago/Turabian StyleCazzin, Stefania, Nicole Liechti, Damian Jandrasits, Eleonora Flacio, Christian Beuret, Olivier Engler, and Valeria Guidi. 2023. "First Detection of West Nile Virus Lineage 2 in Mosquitoes in Switzerland, 2022" Pathogens 12, no. 12: 1424. https://doi.org/10.3390/pathogens12121424