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Article

Proteomic Analysis of Leishmania donovani Membrane Components Reveals the Role of Activated Protein C Kinase in Host-Parasite Interaction

ICMR-National Institute of Pathology, Safdarjung Hospital Campus, New Delhi 110029, India
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Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Lawrence S. Young
Pathogens 2021, 10(9), 1194; https://doi.org/10.3390/pathogens10091194
Received: 8 April 2021 / Revised: 13 May 2021 / Accepted: 25 May 2021 / Published: 15 September 2021
(This article belongs to the Special Issue Immune Response in Parasite Infections)
Visceral leishmaniasis (VL), mainly caused by the Leishmania donovani parasitic infection, constitutes a potentially fatal disease, for which treatment is primarily dependent on chemotherapy. The emergence of a resistant parasite towards current antileishmanial agents and increasing reports of relapses are the major concerns. Detailed research on the molecular interaction at the host-parasite interface may provide the identification of the parasite and the host-related factors operating during disease development. Genomic and proteomic studies highlighted several essential secretory and cytosolic proteins that play vital roles during Leishmania pathogenesis. The aim of this study was to identify membrane proteins from the Leishmania donovani parasite and the host macrophage that interact with each other using 2-DE/MALDI-TOF/MS. We identified membrane proteins including activated protein C kinase, peroxidoxin, small myristoylated protein 1 (SMP-1), and cytochrome C oxidase from the parasite, while identifying filamin A interacting protein 1(FILIP1) and β-actin from macrophages. We further investigated parasite replication and persistence within macrophages following the macrophage-amastigote model in the presence or absence of withaferin (WA), an inhibitor of activated C kinase. WA significantly reduced Leishmania donovani replication within host macrophages. This study sheds light on the important interacting proteins for parasite proliferation and virulence, and the establishment of infection within host cells, which can be targeted further to develop a strategy for chemotherapeutic intervention. View Full-Text
Keywords: host-parasite interaction; Leishmania donovani; membrane protein; protein-protein interaction; 2-DE host-parasite interaction; Leishmania donovani; membrane protein; protein-protein interaction; 2-DE
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MDPI and ACS Style

Verma, S.; Deep, D.K.; Gautam, P.; Singh, R.; Salotra, P. Proteomic Analysis of Leishmania donovani Membrane Components Reveals the Role of Activated Protein C Kinase in Host-Parasite Interaction. Pathogens 2021, 10, 1194. https://doi.org/10.3390/pathogens10091194

AMA Style

Verma S, Deep DK, Gautam P, Singh R, Salotra P. Proteomic Analysis of Leishmania donovani Membrane Components Reveals the Role of Activated Protein C Kinase in Host-Parasite Interaction. Pathogens. 2021; 10(9):1194. https://doi.org/10.3390/pathogens10091194

Chicago/Turabian Style

Verma, Sandeep, Deepak K. Deep, Poonam Gautam, Ruchi Singh, and Poonam Salotra. 2021. "Proteomic Analysis of Leishmania donovani Membrane Components Reveals the Role of Activated Protein C Kinase in Host-Parasite Interaction" Pathogens 10, no. 9: 1194. https://doi.org/10.3390/pathogens10091194

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