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Brief Report

Transferrins Reduce Replication of Chlamydia suis in McCoy Cells

1
Laboratory for Immunology and Animal Biotechnology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium
2
Laboratory of Cell Biology and Histology, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Wilrijk, Belgium
*
Author to whom correspondence should be addressed.
Shared senior authorship.
Academic Editors: Martina Jelocnik and Hanna Marti
Pathogens 2021, 10(7), 858; https://doi.org/10.3390/pathogens10070858
Received: 14 June 2021 / Revised: 2 July 2021 / Accepted: 4 July 2021 / Published: 7 July 2021
(This article belongs to the Special Issue Animal Chlamydiae: A Concern for Human and Veterinary Medicine)
Chlamydia suis (C. suis) resides in the intestines of pigs and tetracycline-resistant strains are emerging worldwide. Intestinal infections are often subclinical. However, the gut is regarded as a C. suis reservoir and clinical infections have been associated with enteritis, conjunctivitis, pneumonia and reproductive failure. C. suis was found in boar semen and venereal transmission occurred. We studied the anti-Chlamydia suis activity of ovotransferrin (ovoTF) and bovine lactoferrin (bLF). Pre-incubation of C. suis with bLF or ovoTF had no significant effect on overall chlamydia replication (mean fluorescence area) in McCoy cells. The addition of ovoTF to the culture medium had no effect on bacterial replication, but the addition of 0.5 or 5 mg/mL of bLF significantly reduced the inclusion size by 17% and 15% respectively. Egg components are used for cryopreservation of boar semen. When inoculating an ovoTF-containing and Chlamydia suis-spiked semen sample in McCoy cells, a significant reduction in inclusion number (by 7%) and overall replication (by 11%) was observed. Thus, we showed that transferrins possess anti-chlamydial activity. Moreover, ovoTF addition to semen extenders might reduce C. suis venereal transmission. Further research is needed to unravel the mechanisms behind the observations and to enhance the effect of transferrins on C. suis. View Full-Text
Keywords: Chlamydia suis; swine; transferrin; ovotransferrin; lactoferrin Chlamydia suis; swine; transferrin; ovotransferrin; lactoferrin
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MDPI and ACS Style

De Puysseleyr, L.; De Puysseleyr, K.; Rybarczyk, J.; Vander Donck, P.; De Vos, W.H.; Vanrompay, D. Transferrins Reduce Replication of Chlamydia suis in McCoy Cells. Pathogens 2021, 10, 858. https://doi.org/10.3390/pathogens10070858

AMA Style

De Puysseleyr L, De Puysseleyr K, Rybarczyk J, Vander Donck P, De Vos WH, Vanrompay D. Transferrins Reduce Replication of Chlamydia suis in McCoy Cells. Pathogens. 2021; 10(7):858. https://doi.org/10.3390/pathogens10070858

Chicago/Turabian Style

De Puysseleyr, Leentje, Kristien De Puysseleyr, Joanna Rybarczyk, Paulien Vander Donck, Winnok H. De Vos, and Daisy Vanrompay. 2021. "Transferrins Reduce Replication of Chlamydia suis in McCoy Cells" Pathogens 10, no. 7: 858. https://doi.org/10.3390/pathogens10070858

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