Study of the Plasma and Buffy Coat in Patients with SARS-CoV-2 Infection—A Preliminary Report
Abstract
:1. Introduction
2. Results
2.1. Clinical Findings
2.2. RNA Plasma Findings
2.3. RNA Buffy Coat Findings
2.4. Morphological and Immunohistochemical Buffy Coat Findings
2.4.1. Morphological Study
2.4.2. Immunohistochemical Study
3. Discussion
4. Materials and Methods
4.1. Study Design and Patient Cohort
4.2. Blood Samples
4.3. RNA Plasma Extraction
4.4. RNA Buffy Coat Extraction
4.5. Histopathological Preparation of the Buffy Coat
4.6. Immunohistochemistry Study of the Buffy Coat
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patient | N | % |
---|---|---|
Male | 18 | 75 |
Female | 6 | 25 |
Symptoms | 22 | 91.67 |
Dry cough | 21 | 95.45 |
Fever | 21 | 95.45 |
Dyspnea | 20 | 90.91 |
Odynophagia | 15 | 68.18 |
Anosmia | 6 | 68.18 |
GI symptoms | 1 | 4.55 |
Asymptomatic | 2 | 8.33 |
Intensive care unit | ||
Required | 13 | 54.17 |
Not required | 11 | 45.83 |
Lived | 16 | 66.67 |
Died | 8 | 33.33 |
Patient | Gender | Age (Years) | Comorbidities | Symptoms | SARS-CoV-2 ** | Initial O2 Sat. (%) | C-Reactive Protein (mg/dL) | X-ray Diagnostic (Lung) | Evolution |
---|---|---|---|---|---|---|---|---|---|
1 | Male | 69 | DLP, CRCa | Cough, Fever, Dyspnea | Positive | 91 | 12.78 | Bilateral basal condensation | Live |
2 | Female | 77 | SAH | Cough, Fever, Dyspnea | Positive | 95 | 22.2 | Left basal condensation | Died |
3 | Male | 51 | Hyperglycemia | Fever, Dyspnea | Positive | 94 | 17.6 | Bilateral basal condensation | Live |
4 | Male | 54 | SAH, T2DM, DLP | Cough, Fever | Positive | 95 | 15.7 | Bilateral alveolar infiltrates | Live |
5 | Female | 58 | BRCa | Cough, Fever, Dyspnea | Positive | 90 | 30 | Bilateral peripheral infiltrates | Live |
6 | Female | 54 | Hypothyroidism | Cough, Fever, Dyspnea | Positive | 87 | 14.72 | Bilateral interstitial infiltrates | Live |
7 | Male | 72 | SAH, T2DM, DLP | Cough, Dyspnea | Positive | 95 | 28 | Bilateral interstitial infiltrates | Died |
8 | Male | 78 | SAH | Cough, Fever, Dyspnea | Positive | 93 | 4.85 | Bilateral interstitial infiltrates | Died |
9 | Male | 63 | T2DM | Fever, Dyspnea | Positive | 70 | 11 | Bilateral basal condensation | Live |
10 | Female | 73 | SAH, DLP | Cough, Fever, Dyspnea | Positive | 98 | NR | Bilateral interstitial infiltrates | Live |
11 | Male | 62 | SAH, T2DM, DLP | Fever, Dyspnea | Positive | 85 | 22 | Bilateral interstitial infiltrates | Died |
12 | Male | 62 | SAH, T2DM, CRI | Cough, Fever, Dyspnea | Positive | 90 | 18.7 | Bilateral interstitial infiltrates | Died |
13 | Male | 57 | SAH, DLP | Cough, Dyspnea | Positive | 65.8 | 13 | Bilateral interstitial infiltrates | Live |
14 | Male | 69 | SAH, DLP | Cough, Dyspnea | Positive | 65 | 16 | Bilateral basal condensation | Live |
15 | Male | 61 | No | Cough, Fever, Dyspnea | Positive | 75 | 25 | Bilateral basal condensation | Live |
16 | Male | 54 | DLP | Cough, Fever, Dyspnea | Positive | 92 | 6.31 | Bilateral interstitial infiltrates | Live |
17 | Male | 69 | No | Cough, Fever, Dyspnea | Positive | 90 | 14.3 | Bilateral basal condensation | Live |
18 | Female | 76 | CRI | Diarrhea | Positive | 97 | 10 | Right basal condensation | Died |
19 | Male | 82 | SAH, DLP | Cough, Dyspnea | Positive | 88 | 4.2 | Bilateral interstitial infiltrates | Died |
20 | Female | 63 | SAH, T2DM, DLP | Cough, Fever, Dyspnea | Positive | 90 | 16.93 | Bilateral basal condensation | Live |
21 | Male | 53 | SAH, DLP | Asymptomatic | Positive | 98 | NR | No infiltrates. No consolidations | Live |
22 | Male | 54 | SAH, Obesity | Asymptomatic | Positive | 98 | NR | No infiltrates. No consolidations | Live |
23 | Male | 51 | DLP | Cough, Fever, Dyspnea | Positive | 94 | 13 | Bilateral interstitial infiltrates | Live |
24 | Male | 77 | SHA, DLP | Cough, Fever, Dyspnea | Positive | 88 | 6.14 | Bilateral basal condensation | Died |
Control | |||||||||
1 | Male | 72 | CRCa | Asymptomatic | Negative | - | - | - | Live |
2 | Male | 52 | LCa | Asymptomatic | Negative | - | - | - | Live |
3 | Male | 51 | LCa | Asymptomatic | Negative | - | - | - | Live |
4 | Female | 62 | CRCa | Asymptomatic | Negative | - | - | - | Live |
5 | Female | 62 | LCa | Asymptomatic | Negative | - | - | - | Live |
6 | Male | 75 | CRCa | Asymptomatic | Negative | - | - | - | Live |
7 | Female | 64 | CRCa | Asymptomatic | Negative | - | - | - | Live |
8 | Female | 68 | LCa | Asymptomatic | Negative | - | - | - | Live |
Case | Age | Sex | Gene Plasma | Gene Buffy Coat | Evolution |
---|---|---|---|---|---|
2 | 77 | F | ORF, S, N | ORF, N | Died |
4 | 54 | M | N | N | Live |
5 | 58 | F | ORF, S | Negative | Live |
6 | 54 | F | ORF, N, S | N, S | Live |
7 | 72 | M | ORF | Negative | Died |
8 | 78 | M | ORF, S | Negative | Died |
9 | 63 | M | ORF, S | Negative | Live |
13 | 57 | M | ORF, S | Negative | Live |
14 | 69 | M | ORF, S | Negative | Live |
17 | 69 | M | ORF, N, S | ORF, N | Live |
18 | 76 | F | ORF | Negative | Died |
19 | 82 | M | ORF, S | Negative | Live |
23 | 77 | M | ORF, S | Negative | Died |
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Peña, K.B.; Riu, F.; Gumà, J.; Guilarte, C.; Pique, B.; Hernandez, A.; Àvila, A.; Parra, S.; Castro, A.; Rovira, C.; et al. Study of the Plasma and Buffy Coat in Patients with SARS-CoV-2 Infection—A Preliminary Report. Pathogens 2021, 10, 805. https://doi.org/10.3390/pathogens10070805
Peña KB, Riu F, Gumà J, Guilarte C, Pique B, Hernandez A, Àvila A, Parra S, Castro A, Rovira C, et al. Study of the Plasma and Buffy Coat in Patients with SARS-CoV-2 Infection—A Preliminary Report. Pathogens. 2021; 10(7):805. https://doi.org/10.3390/pathogens10070805
Chicago/Turabian StylePeña, Karla B., Francesc Riu, Josep Gumà, Carmen Guilarte, Berta Pique, Anna Hernandez, Alba Àvila, Sandra Parra, Antoni Castro, Conxita Rovira, and et al. 2021. "Study of the Plasma and Buffy Coat in Patients with SARS-CoV-2 Infection—A Preliminary Report" Pathogens 10, no. 7: 805. https://doi.org/10.3390/pathogens10070805
APA StylePeña, K. B., Riu, F., Gumà, J., Guilarte, C., Pique, B., Hernandez, A., Àvila, A., Parra, S., Castro, A., Rovira, C., Cueto, P., Vallverdu, I., & Parada, D. (2021). Study of the Plasma and Buffy Coat in Patients with SARS-CoV-2 Infection—A Preliminary Report. Pathogens, 10(7), 805. https://doi.org/10.3390/pathogens10070805