Direct Evidence for Viral Antigen Presentation during Latent Cytomegalovirus Infection
Abstract
1. Introduction
2. Materials and Methods
2.1. Mice, Viruses, and Establishment of Latent Infection
2.2. Peptides and Quantitation of Functional Epitope-Specific CD8+ T Cells
2.3. Cell Transfer and in Vivo Proliferation Assay
3. Results and Discussion
3.1. Epitope-Specific MI in C57BL/6 Mice Latently Infected with Recombinant mCMVs Expressing Antigenic Peptide SIINFEKL or its Non-Antigenic Analog SIINFEKA
3.2. Antigen Presentation Is Essential for Driving OT-I Proliferation in Latently Infected Cell Transfer Recipients
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Lemmermann, N.A.W.; Reddehase, M.J. Direct Evidence for Viral Antigen Presentation during Latent Cytomegalovirus Infection. Pathogens 2021, 10, 731. https://doi.org/10.3390/pathogens10060731
Lemmermann NAW, Reddehase MJ. Direct Evidence for Viral Antigen Presentation during Latent Cytomegalovirus Infection. Pathogens. 2021; 10(6):731. https://doi.org/10.3390/pathogens10060731
Chicago/Turabian StyleLemmermann, Niels A. W., and Matthias J. Reddehase. 2021. "Direct Evidence for Viral Antigen Presentation during Latent Cytomegalovirus Infection" Pathogens 10, no. 6: 731. https://doi.org/10.3390/pathogens10060731
APA StyleLemmermann, N. A. W., & Reddehase, M. J. (2021). Direct Evidence for Viral Antigen Presentation during Latent Cytomegalovirus Infection. Pathogens, 10(6), 731. https://doi.org/10.3390/pathogens10060731