Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes

Chlamydia abortus infects livestock species worldwide and is the cause of enzootic abortion of ewes (EAE). In Europe, control of the disease is achieved using a live vaccine based on C. abortus 1B strain. Although the vaccine has been useful for controlling disease outbreaks, abortion events due to the vaccine have been reported. Recently, placental pathology resulting from a vaccine type strain (vt) infection has been reported and shown to be similar to that resulting from a natural wild-type (wt) infection. The aim of this study was to extend these observations by comparing the distribution and severity of the lesions, the composition of the predominating cell infiltrate, the amount of bacteria present and the role of the blood supply in infection. A novel system for grading the histological and pathological features present was developed and the resulting multi-parameter data were statistically transformed for exploration and visualisation through a tailored principal component analysis (PCA) to evaluate the difference between them. The analysis provided no evidence of meaningful differences between vt and wt strains in terms of the measured pathological parameters. The study also contributes a novel methodology for analysing the progression of infection in the placenta for other abortifacient pathogens. View Full-Text

Keywords: enzootic abortion of ewes; Chlamydia abortus 1B vaccine strain; ovine placenta; principal component analysis; histology; immunohistochemistry

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MDPI and ACS Style

Caspe, S.G.; Palarea-Albaladejo, J.; Underwood, C.; Livingstone, M.; Wattegedera, S.R.; Milne, E.; Sargison, N.D.; Chianini, F.; Longbottom, D. Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes. Pathogens 2021, 10, 543. https://doi.org/10.3390/pathogens10050543

AMA Style

Caspe SG, Palarea-Albaladejo J, Underwood C, Livingstone M, Wattegedera SR, Milne E, Sargison ND, Chianini F, Longbottom D. Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes. Pathogens. 2021; 10(5):543. https://doi.org/10.3390/pathogens10050543

Chicago/Turabian Style

Caspe, Sergio Gastón, Javier Palarea-Albaladejo, Clare Underwood, Morag Livingstone, Sean Ranjan Wattegedera, Elspeth Milne, Neil Donald Sargison, Francesca Chianini, and David Longbottom. 2021. "Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes" Pathogens 10, no. 5: 543. https://doi.org/10.3390/pathogens10050543

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Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes

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