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Brief Report

Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report

1
IHU Méditerranée Infection, 13005 Marseille, France
2
Aix-Marseille-Université, IRD, MEPHI, IHU Méditerranée Infection, 13005 Marseille, France
3
Department of Neurosurgery, University Hospital of Marseille, 13015 Marseille, France
*
Author to whom correspondence should be addressed.
Academic Editor: Natasha N. Gaudreault
Pathogens 2021, 10(4), 461; https://doi.org/10.3390/pathogens10040461
Received: 12 March 2021 / Revised: 5 April 2021 / Accepted: 9 April 2021 / Published: 12 April 2021
Current routine real-time PCR methods used for the point-of-care diagnosis of infectious meningitis do not allow for one-shot genotyping of the pathogen, as in the case of deadly Haemophilus influenzae meningitis. Real-time PCR diagnosed H. influenzae meningitis in a 22-year-old male patient, during his hospitalisation following a more than six-metre fall. Using an Oxford Nanopore Technologies real-time sequencing run in parallel to real-time PCR, we detected the H. influenzae genome directly from the cerebrospinal fluid sample in six hours. Furthermore, BLAST analysis of the sequence encoding for a partial DUF417 domain-containing protein diagnosed a non-b serotype, non-typeable H.influenzae belonging to lineage H. influenzae 22.1-21. The Oxford Nanopore metagenomic next-generation sequencing approach could be considered for the point-of-care diagnosis of infectious meningitis, by direct identification of pathogenic genomes and their genotypes/serotypes. View Full-Text
Keywords: bacterial meningitis; point-of-care diagnostic; Haemophilus influenzae; Oxford Nanopore Technologies; real-time sequencing; metagenomic next-generation sequencing bacterial meningitis; point-of-care diagnostic; Haemophilus influenzae; Oxford Nanopore Technologies; real-time sequencing; metagenomic next-generation sequencing
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MDPI and ACS Style

Morsli, M.; Kerharo, Q.; Delerce, J.; Roche, P.-H.; Troude, L.; Drancourt, M. Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report. Pathogens 2021, 10, 461. https://doi.org/10.3390/pathogens10040461

AMA Style

Morsli M, Kerharo Q, Delerce J, Roche P-H, Troude L, Drancourt M. Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report. Pathogens. 2021; 10(4):461. https://doi.org/10.3390/pathogens10040461

Chicago/Turabian Style

Morsli, Madjid, Quentin Kerharo, Jeremy Delerce, Pierre-Hugues Roche, Lucas Troude, and Michel Drancourt. 2021. "Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report" Pathogens 10, no. 4: 461. https://doi.org/10.3390/pathogens10040461

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