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Article

Updates on Geographical Dispersion of Leishmania Parasites Causing Cutaneous Affections in Algeria

by
Arezki Izri
1,2,†,
Amina Bendjaballah-Laliam
3,†,
Denis Sereno
4,5,‡ and
Mohammad Akhoundi
1,*,‡
1
Parasitology-Mycology Department, Avicenne Hospital, AP-HP, 93009 Bobigny, France
2
Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), 13005 Marseille, France
3
Etablissement Public Hospitalier de Hadjout, Tipaza 42200, Algeria
4
MIVEGEC, Institut de Recherche pour le Développement, Montpellier University, 34394 Montpellier, France
5
InterTryp, Institut de Recherche pour le Développement, Montpellier University, 34398 Montpellier, France
*
Author to whom correspondence should be addressed.
†
Equal contribution.
‡
Equal contribution.
Pathogens 2021, 10(3), 267; https://doi.org/10.3390/pathogens10030267
Submission received: 20 January 2021 / Revised: 18 February 2021 / Accepted: 23 February 2021 / Published: 25 February 2021
(This article belongs to the Special Issue The Neglected Tropical Diseases (NTD))
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Abstract

Leishmaniases are neglected tropical diseases of public health concern in Algeria. To update the geographical distribution of Leishmania spp. causing cutaneous affection, we examined a set of Giemsa-stained smears prepared from skin lesions of the patients suspected to have cutaneous leishmaniasis (CL) in various geographical areas in Algeria. The identification of Leishmania parasites was performed using microscopy, conventional PCR, and PCR–RFLP (PCR-Restriction Fragment Length Polymorphism) targeting ITS1-rDNA. Among 32 smears provided from 27 suspected patients with cutaneous lesions, no trace of parasites was observed in the smear of three patients using microscopy and molecular approaches. Furthermore, four patients presented at least two lesions. PCR–RFLP confirmed the presence of Leishmania in 29 smears prepared from 24 patients. Two biopsies, negative after microscopic examination, were found positive by PCR. Of these 29 PCR positive smears (24 patients), 20 were identified using RFLP–PCR as L. major, two as L. tropica, and two as L. infantum. We found L. major infected patients from Ain skhouna, Biskra, El M’hir, Ghardaïa, M’Sila, and Saida, in agreement with previously reported cases. Furthermore, we highlighted for the first time, the identification of L. major in the patients from Bourkika, Bou Kremissa, Bou Saada Clef, Hajout, Maghnia, Médéa, Menaceur, Messad, Mostaghanem, Nador, Oran, and Sidi Okba. A phylogenetic reconstruction performed with sequences collected from the PCR products confirmed these identifications. Our data provide additional information on the geographical extension of CL caused by L. tropica and L. infantum in Algeria.

1. Introduction

Leishmaniases are vector-borne diseases caused by obligate protozoan parasites from the genus Leishmania (Trypanosomatida: Trypanosomatidae), and transmitted by the bite of infected female phlebotomine sandflies (Diptera: Psychodidae), whose hosts/reservoirs are animals such as canids, rodents, marsupials, hyraxes, or humans [1]. Epidemiological cycles of leishmaniases fall into two broad categories: the zoonotic forms of leishmaniases (ZL), where the primary reservoirs are wild or domestic mammals, and anthroponotic forms (AL) for which humans are the primary reservoirs. Two clinical presentations are distinguished: visceral (VL) and cutaneous (CL). Leishmaniases are endemic in large areas of the tropics, subtropics, and the Mediterranean basin. In 2018, 92 and 83 countries or territories were considered endemic or previously reported for CL and VL, respectively [2]. There are approximately 350 million people at risk for leishmaniases, and about 12 million infected cases worldwide, with an estimated annual incidence of 0.7–1.2 million for CL, and 0.2–0.4 million for VL (https://www.who.int/health-topics/leishmaniasis). Seven countries (Brazil, Ethiopia, India, Kenya, Somalia, South Sudan, and Sudan) report a high VL burden, and 10 countries (Afghanistan, Algeria, Bolivia, Brazil, Colombia, Iran, Iraq, Pakistan, Peru, Syria, and Tunisia) a high CL burden [3,4].
In the Mediterranean basin, leishmaniases are neglected diseases that are emerging or re-emerging [5,6]. Algeria belongs to the shortlist of the most affected countries for leishmaniasis, with more than 20,000 cases reported each year, and an incidence of 28.19 cases per 100,000 inhabitants [3]. Zoonotic visceral leishmaniasis (ZVL) is caused by Leishmania infantum, with dogs acting as the main reservoir and Phlebotomus longicuspis and P. perniciosus acting as primary vectors [7]. Historically present mainly in the humid and sub-humid regions of northern Algeria, it has extended from its historical foci of Kabylie (Tizi-Ouzou, Bejaïa) to Blida, Chlef, Medea, and Tipaza foci. The highest number of reported cases occurred in 1998 (310 reported cases); an overall increase recorded from 1994 to 2003 was followed by a decrease during the subsequent decade [8]. In Algeria, cutaneous leishmaniasis (CL) caused by L. major, L. infantum, and L. tropica has a 30-fold higher incidence than the visceral form [8]. Zoonotic cutaneous leishmaniasis (ZCL) is caused by L. major, in which the proven vector and reservoir are Phlebotomus papatasi and Psammomys obesus, respectively [9]. The disease is prevalent in 41 out of Algeria’s 48 districts, spanning the North Saharan fringe, and the arid and semi-arid bioclimatic areas, including Biskra, Bordj Bou Arreridj, Batna, Djelfa, Saida, Sétif, M’sila, and Abadla [9,10]. More recently, a spread of the disease has taken place towards M’sila, Ksar Chellala, Djelfa, and Bou-Saada foci [11], and the Northern part of the Tell Atlas, in the Soummam basin [12]. Leishmania tropica causes anthroponotic cutaneous leishmaniasis (ACL), a chronic form with less than 100 cases per year that commonly occurs in sympatry with L. major [13,14]. It is restricted to Constantine, Annaba, Ghardaia, and Tipaza [13,15,16]. Phlebotomus sergenti is considered the proven vector of L. tropica, with humans as the primary reservoir. Nevertheless, some animals like Massoutiera mzabi (the Mzab gundi from the family Ctenodactylidae) are additional suspected reservoirs [17,18,19]. Sporadic cutaneous leishmaniasis caused by L. infantum was first reported by Sergent in 1923 [20]. The parasitological, epidemiological, and clinical characteristics were individualized by Belazzoug et al. (1985) [21]. Izri and Belazzoug (1993) [22] highlighted the vectorial role of P. perfiliewi in Ténès. It is responsible for sporadic cutaneous infections all over the coastal regions in northwestern Algeria (Oran, Tlemcen) [7,8,23] and the Algerian Tell Atlas (Tizi-Ouzou, Bouira, Bord Menail, Tipaza, Blida, and Algiers) [24].
Herein, we diagnosed and identified Leishmania spp. from suspect CL patients originating from Algeria’s geographical areas. This allowed us to update the geographical distribution of Leishmania sp. causing cutaneous infections in Algeria.

2. Materials and Methods

2.1. Samples and Clinic

The investigation was conducted from Jun 2016 to November 2017 on patients with symptoms reminiscent of cutaneous leishmaniasis, referred to the Hadjout, Biskra, and Saida health centers. The personal information and lesion type (wet or dry), number, location, duration, and travel history were recorded for each patient. Cutaneous biopsies, sampled according to Evans’s protocol [25], were smeared on a microscopic slide, air-dried, fixed with absolute methanol, stained by Giemsa 10% (Sigma-Aldrich, Saint Louis, MO, USA), and directly examined under a light microscope at 500× or 1000× magnification.

2.2. Molecular Diagnosis and Typing

The DNA from stained slides was extracted using a Qiagen DNA mini-kit (Hilden, Germany) and precipitated by ethanol [26]. A conventional polymerase chain reaction (PCR) that amplifies a 300–350 bp fragment (depending on the species) of the internal transcribed spacer 1 (ITS1) was performed using LITSR (forward: 5′-CTGGATCATTTTCCGATG-3′) and L5.8S (reverse: 5′-TGATACCACTTATCGCACTT-3′) primers [27]. Negative (absence of target DNA) and positive (presence of DNA from reference Leishmania strains) controls were used for each PCR batch. Amplicons were analyzed after electrophoresis in a 1.5% agarose gel containing ethidium bromide. Endonuclease digestion was performed following a previously published protocol [27]. Briefly, 10 μL of the PCR product was incubated at 37 °C in a final volume of 30 μL, containing 2 μL of BsuRI (HaeIII) (Fermentas, Vilnius, Lithuania), 2 μL of 10× buffer, and 16 μL of distilled water. After 4 h, digested fragments were run on a 3% agarose gel containing ethidium bromide. A DNA ladder of 50 bp (Fermentas) was used to identify diagnostic DNA fragments.

2.3. Sequencing and Typing of Leishmania Isolates

Leishmania DNA was subjected to conventional PCR targeting ITS1 (partial sequence), 5.8S (complete sequence), and ITS2 (partial sequence), using forward (ITS1F: 5′-GCAGCTGGATCATTTTCC-3′) and reverse (ITS2R4: 5′-ATATGCAGAAGAGAGGAGGC-3′) primers with an expected length of 430 bp [28,29]. Double-distilled water and purified DNA from L. major, L. tropica, and L. infantum were used as negative and positive controls for each PCR batch. Amplicon quality was analyzed after electrophoresis in a 1.5% agarose gel with ethidium bromide. PCR products were purified using an Invisorb Fragment CleanUp kit (Stratec Molecular, Berlin, Germany) and sequenced using the same primers for PCR amplification. The sequences were compared to homologous sequences collected in the GenBank database and aligned with the Basic Local Alignment Search Tool (BLAST) (www.ncbi.nlm.nih.gov/BLAST). All sequences were identified as L. major, L. tropica, or L. infantum, based on ≥99% identity with GenBank sequences. The phylogenetic analysis was carried out using MEGA v.6 software. A phylogenetic tree of Leishmania species (identified in this study) and GenBank sequences was constructed using neighbor-joining (NJ) with bootstrap values of 1000 replicates.

3. Results

A total of 32 Giemsa stained smears were prepared from active skin lesions of suspected 27 CL patients referred to the Hadjout, Biskra, and Saida health centers in Algeria (Figure 1). Biopsies were taken from all lesions (one to three lesions) from patients of ages ranging from 3 to 82. After microscopic examination, 27 smears from the 32 lesions processed were positive for Leishmania sp. (including four patients with at least two lesions). Five patients were negative for Leishmania infection after a microscopic examination. See Table 1 for epidemiological and clinical information of all patients.
All biopsies were subjected to molecular characterization by PCR–RFLP. A schematic representation of the PCR–RFLP restriction profile is given in Figure 2, along with the restriction profiles generated for selected samples. The twenty-seven smears (24 patients), which were positive after microscopic examination, were also positive for PCR (Table 1). Two lesions, considered as negative after microscopic examination, were positive with PCR. Most lesions caused by L. major were located on feet (9/20 cases), whereas lesions due to L. tropica were on the head (forehead and face) (Table 1).
The identification of Leishmania at the species level was further confirmed by direct sequencing of each isolate’s PCR product. All the sequences were deposited in GenBank under the accession numbers of XN348129 to XN348154. This analysis pinpoints that Leishmania sequences from Algerian patients clustered into three well-differentiated and supported clades of L. major, L. tropica, and L. infantum (Figure 3). They gathered with Leishmania sequences of various Mediterranean origins collected from GenBank. The two L. infantum sequences clustered with L. infantum isolated from humans or dogs in different Mediterranean countries, with a bootstrap value of 65% (Figure 3).

4. Discussion

The first reported cases of cutaneous and visceral leishmaniases in Algeria date back to 1860 by Hamel, and 1911 by Lemaire [30]. Besides, Edmond and Etienne Sergent and their collaborators were the first, in 1921, to prove sandflies’ vector role. They incriminated the phlebotomus papatasi as transmitting the “Clou de Biskra” agent [31,32]. For a long time, L. major and L. infantum foci were geographically separated in Algeria by the Tell Atlas Mountains, representing a natural barrier. The leishmaniasis epidemiological features seem to be in continuous evolution, resulting in more reports [33].
ZCL due to L. major is the oldest leishmaniasis, with Biskra in the east and Abadla in the west as the formerly known foci in Algeria [34]. It is prevalent over the entire North-Saharan fringe, corresponding to the arid and semi-arid areas with a progression towards the North. Three CL outbreaks occurred between 2004 and 2006, with 14,822, 25,511, and 14,714 cases, respectively. Besides Biskra and Ababla, Msila experienced an epidemic in 1982, with 8000 recorded cases [35]. In recent years, several new foci of CL due to L. major, namely those of El M’hir, Batna, and Bordj Bou Arreridj have emerged on the Northern part of the chain of the Tell Atlas [12,33]. In the present study, in agreement with previously reported cases, we found L. major infected patients coming from Ain skhouna, Biskra, El M’hir, Ghardaïa, M’Sila, and Saida [12,13,36,37,38]. Furthermore, we highlight for the first time, the identification of L. major in the patients from Bourkika, Bou Kremissa, Bou Saada, Chlef, Hajout, Maghnia, Médéa, Menaceur, Messad, Mostaghanem, Nador, Oran, and Sidi Okba (Figure 1, Table 1). Due to limited information on the medical records of some patients, together with the multiple trips of some of them to ZCL endemic regions, mostly due to seasonal works or vacations, it is quite difficult to justify the precise location of some patients when infected by Leishmania parasites (Table 1). Nevertheless, these results confirm the extension of L. major in northern Algeria [12]. Studied patients had an age range between 3 to 82 years old, with most lesions located on the feet (45%) (Table 1). Men exhibited the most cutaneous lesions caused by L. major (13 out of 20 cases, 65%). Based on the phylogenic tree, we recorded some slight intraspecific heterogeneity for L. major (AVC03, AVC05, and AVC06, originating from Biskra, Bou Kremissa, and Bou Saada). Such a genetic diversity has also been reported in other L. major endemic regions; Iran [39], Tunisia [40], and Morocco [41]. On the other hand, ZCL has been the subject of multiple studies, mostly isoenzymatic investigations. The characterization of parasites circulating in Algeria using isoenzymatic analysis started in 1981 [42]. Isoenzymatic characterization of L. major, the causative agent of zoonotic cutaneous leishmaniasis, evidenced the zymodeme MON-25 in patients, sandfly vectors (P. papatasi), and animal reservoirs (Psammomys and Meriones) [10,43,44,45]. Some years later, a new and less prevalent zymodeme, the MON-269, was identified. It differs from MON-25 by the PGD (phosphogluconate dehydrogenase) enzymatic system [45] (Table 2).
ACL due to L. tropica has been reported in the southern part of the country, particularly in the Oasis of Ghardaia [13]. The MON-301 and MON-306 zymodemes of Leishmania tropica are restricted to Constantine [15], Ghardaï [10], and Tipaza [16]. They present some intriguing characteristics, like their inherent lower susceptibility towards antimonial-containing drugs [8,46], or the physiopathological alteration recorded in murine infection models [47]. In the present study, we identified two L. tropica cases from Ghardaia and Constantine, which grouped in the same clade with other L. tropica sequences from other Mediterranean countries.
Since the discovery of VL’s first case in 1911, the Kabylie has been known for many years as an active focus of the visceral form in particular. Located in the north of the country, it presents a very large geodiversity, with very contrasting portions, both from a bioclimatic, geomorphological, and vegetation point of view, thus offering very diverse biotopes for the different species of sand flies and animal reservoirs. For many years, the highest number of VL cases registered in Algeria occurred in the region of Tizi ouzou (Kabylie). In the recent years, an extension of VL from the old foci in Kabylie (Tizi-Ouzou, Bejaı¨a) to the center (Blida, Chlef, Medea, Tipaza) and the north-eastern part of northern Algeria, with scattered cases occurring in the West (Oran, Tlemcen) [7] have been recorded. The MON-1 and MON-24 zymodemes of L. infantum were responsible for zoonotic visceral leishmaniasis and sporadic cutaneous leishmaniasis [48]. They were the most frequently characterized zymodemes in patients, sand flies vectors, and animal reservoirs [9,10,21,22,49]. Although the isoenzymatic characterization allows Leishmania species identification, its complexity and prohibitive costs restrict its use in clinical settings [50]. See Table 2 for a synthetic overview of Leishmania zymodemes characterized in Algeria. Although most VL and sporadic CL cases due to L. infantum are primarily reported in humid regions in northern Algeria, L. infantum infection cases are sporadically reported in arid areas [10]. In Algeria, L. infantum is associated with diverse clinical and eco-epidemiological situations that raised genetic diversity concerns. The occurrence of three L. infantum populations was recorded in Algeria, with two clades encompassing the isolates belonging to the zymodeme MON-1, and a third one, with mainly zymodeme MON-24 isolated from cutaneous leishmaniasis cases [51]. Occasionally recombination events and a generation of hybrid genotypes between MON-1 and MON-24/80 in Algeria have been suspected [52]. In the present study, we identified two SCL cases caused by L. infantum in the patients originating from Tizi Ouzu and Cherchell. Due to the restriction in SCL case numbers processed in the present study, our L. infantum sequences clustered tightly with other Mediterranean strains, with no significant heterogeneity (Figure 3).
Parasitological methods (direct examination and in vitro culture) have several limitations regarding their positivity and sensitivity rate. This poor performance of parasitological methods is related to low parasitic load or irregular distribution of amastigotes in lesions [53]. The use of DNA amplification by PCR has allowed Leishmania parasites to be identified, and clarified the taxa’s distribution [4,33,54]. In analyzing the phylogenic tree generated with specimens isolated from Algerian patients, we recorded a high level of genetic homogeneity in the isolates of L. major, L. tropica, and L. infantum, which cluster with their counterparts identified in various Mediterranean basin areas (Figure 3). This confirms the identification performed using PCR–RFLP and agrees with Leishmania genotyping carried out by Gherbi et al. [55], El Baidouri et al. [56], and Schonian et al. [57] using multilocus microsatellite typing (MLMT) on North African specimens.
Table 2. Leishmania zymodemes reported in human, sand fly, and animal reservoirs in Algeria.
Table 2. Leishmania zymodemes reported in human, sand fly, and animal reservoirs in Algeria.
Clinico-Epidemiological FormZymodemesReference
HumanVectorReservoir
ZCLMON-25 (L. major)
MON-269 (L. major)		MON-25 (P. papatasi)
MON-269 (P. papatasi)		MON-25 (Psammomys obesus)
MON-269 (Psammomys obesus, Meriones shawi)		[9,10,45,58,59]
ACLMON-301 (L. tropica)
MON-306 (L. tropica)		 - -[13,45,60]
SCLMON-1 * (L. infantum)
MON-24 * (L. infantum)
MON-80 * (L. infantum)		MON-24 * (P. perfilliewi) -[22,61]
ZVLMON-1 (L. infantum)
MON-24 (L. infantum)
MON-33 (L. infantum)
MON-34 (L. infantum)
MON-77 (L. infantum)
MON-78 (L. infantum)
MON-80 (L. infantum)
MON-281 (L. infantum)		MON-1 (P. perniciosus)
MON-24 (P. perfilliewi)		MON-1 (Canis familiaris, Canis aureus)
MON-24 (Canis familiaris)
MON-34 (Canis familiaris)
MON-77 (Canis familiaris)
MON-80 (Canis familiaris)
MON-281 (Canis familiaris)		[6,61,62,63,64]
*: causing sporadic cases of cutaneous leishmaniasis.

Author Contributions

Conceptualization: A.I., A.B.-L., D.S., and M.A.; Methodology: A.I., A.B.-L., D.S. and M.A., writing—original draft preparation, A.I., D.S. and M.A., writing—review and editing, A.B.-L., D.S. and M.A. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

The study was conducted according to the Declaration of Helsinki’s guidelines and approved by the Institutional Ethics Committee of Avicenne Hospital (protocol code 95/99/AVC/ESA).

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

All data are available in the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

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Pathogens 10 00267 g001
Figure 1. Schematic representation of leishmaniasis endemic regions for L. major, L. tropica, and L. infantum, and the geographical origin of cutaneous samples processed in the present study (red points).
Figure 1. Schematic representation of leishmaniasis endemic regions for L. major, L. tropica, and L. infantum, and the geographical origin of cutaneous samples processed in the present study (red points).
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Pathogens 10 00267 g002
Figure 2. PCR–RFLP of cutaneous biopsies collected in Algeria. (A) Schematic representation of BsuR1 (HaeIII) cut sites in amplified fragments of ITS1-rDNA in Leishmania major, L. tropica, and L. infantum (CLC DNA Workbench 5.2 software); (B) Ethidium bromide-stained agarose gel of HaeIII digested PCR products of Leishmania species extracted from Giemsa stained smears. M: molecular marker (50 bp); Lanes 1–3: undigested reference strains of L. major, L. tropica, and L. infantum; Lanes 4–6: digested L. major, L. tropica, and L. infantum isolated from the patients; Lane 7: negative control. For L. tropica isolates, the 20 bp fragment could not be observed in an agarose gel electrophoresis. The 57 and 60 bp fragments could not be discriminated; only bands at 200 and 60 bp were indicative and distinguished after agarose gel electrophoresis.
Figure 2. PCR–RFLP of cutaneous biopsies collected in Algeria. (A) Schematic representation of BsuR1 (HaeIII) cut sites in amplified fragments of ITS1-rDNA in Leishmania major, L. tropica, and L. infantum (CLC DNA Workbench 5.2 software); (B) Ethidium bromide-stained agarose gel of HaeIII digested PCR products of Leishmania species extracted from Giemsa stained smears. M: molecular marker (50 bp); Lanes 1–3: undigested reference strains of L. major, L. tropica, and L. infantum; Lanes 4–6: digested L. major, L. tropica, and L. infantum isolated from the patients; Lane 7: negative control. For L. tropica isolates, the 20 bp fragment could not be observed in an agarose gel electrophoresis. The 57 and 60 bp fragments could not be discriminated; only bands at 200 and 60 bp were indicative and distinguished after agarose gel electrophoresis.
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Figure 3. Neighbor-joining (NJ) phylogenetic tree constructed based on ITS1-rDNA sequence of Leishmania samples analyzed in the present study (samples entitled AVC) and those collected in GenBank.
Figure 3. Neighbor-joining (NJ) phylogenetic tree constructed based on ITS1-rDNA sequence of Leishmania samples analyzed in the present study (samples entitled AVC) and those collected in GenBank.
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Table 1. Clinical and epidemiological data of patients and Leishmania diagnosis.
Table 1. Clinical and epidemiological data of patients and Leishmania diagnosis.
PatientsLesionsLeishmania Diagnosis Geographical Location Explanation
Patient CodeSexAge (Year)NumberSiteMicroscopyPCR–RFLP/SequencingCityElevation (m) *Rainfall (mm)Bioclimatic Stage **Disease or Travel History
AVC1M391Neck ++/L. majorAin skhouna99587Cold semi-arid
AVC2M302Forearm, hand ++/L. majorBiskra121128Arid (Desertic)
AVC3M411Ankle ++/L. majorBiskra
AVC4F51Face ++/L. majorBourkika203642Mediterranean
AVC5M591Ankle ++/L. majorBou Kremissa328201Cold semi-arid
AVC6F91Foot ++/L. majorBou Saada4898Arid (Desertic)
AVC7M691Forearm ++/L. infantumCherchell26108Mediterranean
AVC8M491Foot ++/L. majorChlef114394MediterraneanDiabetic
AVC9F71Forehead ++/L. tropicaConstantine694512Mediterranean
AVC10F272Foot ++/L. majorEl M’hir619329Mediterranean
AVC11F161Face ++/L. tropicaGhardaïa49768Arid (Desertic)
AVC12F701Foot - -Gouraya670642Mediterranean
AVC13M501Forearm - -Hadjout81635Warm temperate
AVC14M291Hand ++/L. majorHadjout
AVC15M413Foot, hand ++/L. majorHadjout
AVC16M791Ankle ++/L. majorMaghnia374365Warm temperateTravel to Morocco
AVC17F741Forearm ++/L. majorMédéa981736Warm temperateDiabetic, nephrotic disorder
AVC18M101Cheek ++/L. majorMenaceur321661Warm temperate2 months inhabitation in Biskra
AVC19M641Neck ++/L. majorMesaad59269Arid (Desertic)
AVC20F41Foot ++/L. majorMostaganem104347Cold semi-aridTravel to M’Sila
AVC21F32Cheek, foot ++/L. majorM’Sila471229Cold semi-arid
AVC22M181Cheek ++/L. majorNador42313Semi-aridTravel to Biskra
AVC23M821Neck -+/L. majorOran0.9370Warm temperateTravel to Tunisia
AVC24M281Forearm ++/L. majorSaida830341Cold semi-arid
AVC25F361Cheek - -Sidi Ghiles30634Mediterranean
AVC26F141Hand -+/L. majorSidi Okba54127Arid (Desertic)
AVC27M111Foot ++/L. infantumTizi Ouzu200705Mediterranean
M: Male; F: Female; *: meter above sea level; **: Based on Köppen climate classification Csa.
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Izri, A.; Bendjaballah-Laliam, A.; Sereno, D.; Akhoundi, M. Updates on Geographical Dispersion of Leishmania Parasites Causing Cutaneous Affections in Algeria. Pathogens 2021, 10, 267. https://doi.org/10.3390/pathogens10030267

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Izri A, Bendjaballah-Laliam A, Sereno D, Akhoundi M. Updates on Geographical Dispersion of Leishmania Parasites Causing Cutaneous Affections in Algeria. Pathogens. 2021; 10(3):267. https://doi.org/10.3390/pathogens10030267

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Izri, Arezki, Amina Bendjaballah-Laliam, Denis Sereno, and Mohammad Akhoundi. 2021. "Updates on Geographical Dispersion of Leishmania Parasites Causing Cutaneous Affections in Algeria" Pathogens 10, no. 3: 267. https://doi.org/10.3390/pathogens10030267

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Izri, A., Bendjaballah-Laliam, A., Sereno, D., & Akhoundi, M. (2021). Updates on Geographical Dispersion of Leishmania Parasites Causing Cutaneous Affections in Algeria. Pathogens, 10(3), 267. https://doi.org/10.3390/pathogens10030267

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