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Background:
Systematic Review

Using Respiratory Gas Analyzers to Determine Resting Metabolic Rate in Adults: A Systematic Review of Validity Studies

by
César Ulises Olivas-León
1,2,
Francisco Javier Olivas-Aguirre
1,3,
Isaac Armando Chávez-Guevara
1,4,
Horacio Eusebio Almanza-Reyes
1,2,
Leslie Patrón-Romero
1,2,
Genaro Rodríguez-Uribe
1,2,
Francisco José Amaro-Gahete
5,6,7 and
Marco Antonio Hernández-Lepe
1,2,*
1
Conahcyt National Laboratory of Body Composition and Energetic Metabolism (LaNCoCoME), Tijuana 22390, Mexico
2
Faculty of Medicine and Psychology, Autonomous University of Baja California, Tijuana 22390, Mexico
3
Department of Health Sciences, Biomedical Sciences Institute, Autonomous University of Ciudad Juarez, Ciudad Juarez 32310, Mexico
4
Faculty of Sports Ensenada, Autonomous University of Baja California, Ensenada 22800, Mexico
5
Department of Medical Physiology, Faculty of Medicine, Sport and Health University Research Institute (iMUDS), University of Granada, 18001 Granada, Spain
6
CIBER Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, 28029 Granada, Spain
7
Biosanitary Research Institute, Ibs.Granada, 18012 Granada, Spain
*
Author to whom correspondence should be addressed.
Sports 2025, 13(7), 198; https://doi.org/10.3390/sports13070198
Submission received: 7 April 2025 / Revised: 14 June 2025 / Accepted: 18 June 2025 / Published: 22 June 2025
Browse Figure
Review Reports Versions Notes

Abstract

:
Background: Correct assessment of resting metabolic rate (RMR) is fundamental for estimating total energy expenditure in both clinical nutrition and sports sciences research. Various methods have been proposed for RMR determination, including predictive equations, isotopic dilution techniques, and indirect calorimetry. Over the past two decades, portable gas analyzers have emerged as promising alternatives, offering more accessible and cost-effective solutions for metabolic assessment. However, evidence regarding their validity remains inconsistent, particularly across diverse populations and varying metabolic assessment protocols. Methods: This systematic review was conducted in May 2025 using the PubMed, Web of Science, and EBSCO databases, following the PRISMA-DTA guidelines, and included observational studies with the objective of examining the available evidence regarding the validity of portable gas analyzers to determine RMR in humans. The methodological quality of each study was assessed using the NIH Quality Assessment Tool for Observational Cohort and Cross-Sectional Studies. Results: From an initial pool of 230 studies, 16 met the eligibility criteria. The findings revealed notable variability in measurement validity among devices, mainly influenced by device model, population characteristics, and methodological factors. While portable analyzers such as FitMate and Q-NRG exhibited high validity, MedGem exhibited systematic biases, particularly in individuals with higher adiposity, leading to RMR overestimations. Conclusions: The main results demonstrated the critical need for rigorous validation of portable gas analyzers before their implementation in clinical and research settings to ensure their applicability across diverse populations and metabolic assessments.

1. Introduction

The proper assessment of resting metabolic rate (RMR) is essential for estimating total energy expenditure in clinical nutrition and sports sciences research [1]. Indeed, an accurate measure is crucial for (i) developing personalized dietary interventions, (ii) optimizing metabolic health, and (iii) managing conditions such as obesity, diabetes, and malnutrition [2]. In this context, bias in this estimation can lead to inadequate caloric prescriptions, potentially affecting nutritional status, weight management, and clinical outcomes [3]. Consequently, the establishment of robust and standardized methodologies for RMR measurement remains a critical objective in both research and clinical practice [4].
One of the earliest methods developed to determine whole-body metabolic rate was the Douglas bag, which consists of evaluating expired gases collected from an exhaled breath over a specific period in a twill bag lined with vulcanized rubber. It is considered the gold standard for measuring respiratory gas exchange but has some limitations such as a limited sample due to the test time for each subject, air leaks or condensation in the bags, and the lack of a standard reference for validation [5].
Several approaches are commonly used to estimate RMR, including predictive equations, but this method does not provide direct measurements of RMR. Instead, indirect calorimetry is often used to determine RMR, which is considered a reference standard for measuring metabolic gas exchange, as it provides direct measurements of oxygen consumption (VO2) and carbon dioxide production (VCO2) under controlled resting conditions [6,7]. Traditionally, metabolic carts have been used for this purpose using different sensor technologies, calibration procedures, or data-processing algorithms. However, variations in these methodologies make it difficult to standardize indirect calorimetry protocols across different clinical and research settings [8]. Furthermore, metabolic carts require controlled laboratory conditions, limiting their feasibility for widespread use in non-laboratory settings.
To address these constraints, portable gas analyzers have been developed over the past two decades, offering a practical and accessible approach for assessing RMR [9]. However, the validity of these devices has been mostly investigated among athletic populations, where metabolic efficiency is a key performance factor [10,11]. Indeed, Van Hooren et al. [12] recently reported that VO2masterPro underestimated VO2 by an average of ~12%, while PNOĒ overestimated VO2 by an average of ~8.3%, being less accurate than stationary metabolic carts for assessing energy expenditure.
Currently, there is scarce information about the validity of portable gas analyzers in the general population, including in healthy individuals and patients with metabolic disorders. If rigorous validation and standardization are not thoroughly conducted, the use of portable gas analyzers may lead to the misdiagnosis of metabolic dysfunctions, the development of ineffective treatment plans, and the generation of inaccurate research findings. Therefore, this work aimed to systematically examine the available evidence regarding the validity of portable gas analyzers in humans across diverse populations and clinical settings.

2. Materials and Methods

This systematic review was strictly conducted in full accordance with the elements outlined in the Preferred Reporting Items for a Systematic Review and Meta-analysis of Diagnostic Test Accuracy Studies: The PRISMA-DTA Checklist (Supplementary S1) [13]. Furthermore, the protocol and its methodological considerations were registered in the International Prospective Register of Systematic Reviews (PROSPERO) public database (ID: CRD420250652077).

2.1. Eligibility Criteria

The included articles in this work were full-text observational studies that assessed RMR in humans. The specific characteristics of the studies were determined using the following PICO strategy: (i) Participants: Healthy untrained adults (>18 years). (ii) Intervention: Measurement of RMR using different portable gas analyzers. (iii) Comparison: Similarities and differences between multiple devices or across different models/equipment. Outcomes: Validity of portable gas analyzers for assessing RMR.

2.2. Exclusion Criteria

After the removal of duplicates, manuscripts with any of the following characteristics were excluded: (i) studies involving patients with pre-existing cardiorespiratory or metabolic disorders, (ii) studies using predictive equations to estimate RMR, (iii) systematic review articles, abstracts, letters to the editor, and conference proceedings, and (iv) studies that lacked clearly defined protocols in their design for RMR measurement.

2.3. Information Sources

The literature search was conducted using three major scientific databases: PubMed, Web of Science, and EBSCO. The following search string with Boolean operators was applied: Adults AND (“resting metabolic rate” OR “resting energy expenditure” OR “basal energy expenditure” OR “RMR” OR “basal metabolic rate” OR “BMR”) AND (“portable metabolic analyzer” OR “metabolic cart” OR “respiratory gas analyzer” OR “indirect calorimetry” OR “metabolic analyzer”) AND (“comparison” OR “validation” OR “consistency” OR “agreement” OR “reliability” OR “accuracy”) NOT equations. The search was conducted on May 2025 and the retrieved results were further restricted to studies published between 2000 and 2025.

2.4. Data Collection and Evaluation of Methodological Quality/Risk of Bias

Data extraction and verification were conducted independently by two investigators (C.U.O.-L. and M.A.H.-L.). The primary outcomes included bibliographic information, participant age, evaluated devices, measured RMR values, and study conclusions. The methodological quality of each study was assessed using the NIH Quality Assessment Tool for Observational Cohort and Cross-Sectional Studies [14]. The risk of bias was de-fined through providing a value of yes/no to 14 questions, resulting in regular (≤10/14 yes) or low (≥11/14 yes) risk of bias. Methodological quality evaluation was conducted independently by two reviewers (C.U.O.-L. and M.A.H.-L.), and any discrepancies were resolved through consensus.

3. Results

A total of 364 articles were initially retrieved from PubMed (n = 143), Web of Science (n = 106), and EBSCO (n = 115). After removing 158 duplicates, 206 unique studies proceeded to further screening by title, abstract, and keywords. A total of 173 studies were excluded based on predefined criteria: inappropriate study design (systematic reviews, n = 2), irrelevant outcomes (n = 162), animal studies (n = 1), and population characteristics misaligned with inclusion criteria (n = 8). Following this phase, 33 studies were entirely read, excluding 15 additional papers due to either the absence of a standard reference method or the lack of direct RMR assessment. Lastly, 18 studies met inclusion criteria and were included in the final analysis (Figure 1).
The main characteristics of the studies are described in Table 1, where it is shown that the validity of metabolic measurement devices differs across studies, with some demonstrating strong agreement with reference methods and others presenting systematic biases influenced by population characteristics.
Finally, the methodological quality/risk of bias of the selected studies is described in Table 2. Most of them showed a “Regular” risk of bias (n = 11), while only 7 were classified as having a “low” risk of bias. Overall, scores related to population sampling, recruitment of a representative sample, and repeated assessment of the exposure factor (evaluation using gas analyzers) were low in most studies.
Devices such as FitMate, Q-NRG, and the Pocket-Sized Metabolic Analyzer have shown high accuracy in assessing VO2 and VCO2 when compared to the Douglas Bag method, a widely recognized gold standard for metabolic gas analysis. Notably, the Q-NRG and FitMate demonstrated high accuracy when comparing their VO2 and VCO2 measurements to gold-standard methodologies, such as the Douglas Bag method. However, some devices exhibit systematic biases, particularly in populations with varying levels of adiposity and body composition, which can complicate their applicability in a broader clinical context. For instance, MedGem, while widely used due its portability and ease of use, tends to overestimate RMR in individuals with obesity, potentially due its simplified measurement algorithm failing to capture metabolic variability within heterogeneous populations [15,30]. In contrast, FitMate has shown high reproducibility and agreement with gold-standard methods. Nieman et al. [22], reported no statistically significant differences between FitMate and the Douglas Bag method for VO2 measurements (242 ± 49 mL/min vs. 240 ± 49 mL/min, p = 0.066) and RMR (1662 ± 340 kcal/day vs. 1668 ± 344 kcal/day, p = 0.579), reinforcing its reliability.
While the Douglas Bag method remains the gold standard for metabolic analysis, its use in validation studies is limited. Only 16.66% of the reviewed studies [22,32] employed the Douglas Bag method, with 83.33% relying on metabolic carts such as Delta-Trac and Quark RMR.
Additionally, variations in measurement protocols, including subject positioning (seated vs. supine) and evaluations duration (ranging from 5 to 30 min), introduce further discrepancies across studies. Differences in pre-test resting time and measurement duration, often dictated by manufacturer recommendations, may contribute to inconsistencies in reported RMR values. The Q-NRG device, compared to Delta-Trac, Quark RMR, and V-max, reported a shorter measurement time and showed consistency in RMR measurements across sessions, reinforcing its potential for repeated assessments. However, its performance may be influenced by factors such as the use of the face mask vs. the canopy hood [25].

4. Discussion

The present systematic review was designed to examine the existing evidence regarding the reliability, validity, and accuracy of portable gas analyzers in humans. The results show only 18 studies that present scientific evidence of good methodological quality across diverse populations and clinical settings using a reference standard, resulting in significant differences in the reliability, validity, and accuracy of portable gas analyzers in measuring different metabolic parameters across various devices and study populations.
Zhao et al. [32] reported a strong correlation between the Pocket-Sized Metabolic Analyzer and the Douglas Bag method, although an approximate 10% variation in results was observed. While this discrepancy is relatively small, it highlights the potential influence of minor differences in device calibration and methodology.
Studies such as Frankenfield & Coleman [19] and Compher et al. [16] indicated that MedGem systematically overestimated RMR in individuals with obesity when compared to Delta-Trac, a widely used metabolic cart. Interestingly, this overestimation was not observed in non-obese individuals, suggesting that body composition significantly influences MedGem’s accuracy.
Other devices, such as FitMate GS and Q-NRG, have demonstrated variability in accuracy when compared to whole body calorimetry and other metabolic carts. In particular, Purcell et al. [24] reported that FitMate GS underestimated RMR, although it exhibited high reproducibility across sessions, which supports its potential use in settings where consistency is prioritized over absolute accuracy. Additionally, Dupertuis et al. [26] found that Q-NRG’s accuracy depends on the use of a canopy hood or a face mask, with the hood mode providing more reliable VO2 and VCO2 measurements than the mask mode, which overestimated RMR in men. This variability emphasizes the importance of selecting the appropriate application mode when using Q-NRG in clinical or research settings.
Of the included studies only three used the Douglas Bag (gold standard) as the validation method for metabolic analysis, with a total of 15 of the included studies relying on metabolic carts as the validation method. While these devices are commonly used, their inherent variability in accuracy raises concerns regarding cross-device standardization and comparability [33,34]. The limited use of the Douglas Bag method highlights the need for further validation studies using gold-standard techniques to improve measurements consistency.
There were only two studies found with populations with risk factors of metabolic diseases; Purcell et al. [24] reported differences in evaluated RMR in normal/overweight subjects compared to obesity, and Hlynsky et al. [17] evaluated women with anorexia, reporting statistical differences of gas analyzers at comparing this population with a control group. It is important to mention that it has been reported that metabolic diseases can affect RMR by either increasing or decreasing it, making evaluation difficult [35], and there exists a need to implement studies focused on describing the best equipment or method to avoid bias when evaluating energetic metabolism in subjects with metabolic diseases.
Currently, there are various devices on the market claiming to be of high quality for assessing metabolic gas exchange; however, there is little evidence of their validity and reproducibility, and the existing evidence is focused on specific populations such as high-performance athletes and hospitalized patients. An example of this is the BIOPAC GASSYS3, which presents significant differences in its measurements for RMR in specific populations, requiring the development of equations to correct the data, which calls into question its validity as an assessment device [36].
Devices such as Delta-Trac and VO2000 have proven their validity compared to the standard methods; however, their manufacture is discontinued, making them impossible to acquire. Likewise, there are devices that have implemented technological improvements compared to previous versions, such as Cortex MetaMax 3B or Cosmed K5. Although these updates are nearly a decade old, these devices remain reliable, but low demand and high costs could hamper the development of new devices of this type [37]. In addition, several portable systems have been developed that are designed to provide basic VO2 measurement and RMR estimation. However, these devices have limitations, as they only provide O2 analysis and require assumptions about RMR. These portable devices are unlikely to be accepted in high-quality research where direct measures of these variables are required [38].
The principal limitations of the discussed documents include the low sample size (≤60 participants in 77.8% of the included studies), the large variation in the ages of the participants, and the fact that 44.44% used the gas analyzer Delta-Trac as the validation method; this device has been discontinued so is not available to be obtained in the market. However, additional variables applicable in both clinical and sports settings could be explored for metabolic evaluations purposes, such as VO2max or maximal fat oxidation, not only variables related to RMR [39]. Furthermore, because the included studies used distinct metrics to represent the validity of the evaluated devices (i.e., mean differences, LoA, ICC, etc.), this made it difficult to compare the results of the studies. Moreover, it was not possible to pool all the data in a robust meta-analysis due to the lack of available data for the Pocket-Sized Metabolic Analyzer, IIM-IC-100 VO2000, and Ecovx Beacon. Finally, the analyzed findings suggest that the characteristics of the studied populations, the technology of each device, the type of measurement, the sampling method, the test range, and the calibration method can influence the accuracy of device measurements, highlighting the importance of conducting studies following the statistical guidelines of Hopkins et al. [40] in diverse populations to standardize the assessment of the validity of portable gas analyzers.

5. Conclusions

This review underscores the variability in reliability and accuracy among respiratory gas analyzers, emphasizing the need for rigorous validation studies. While some devices align closely with gold-standard methods, others demonstrate systematic biases influenced by population characteristics and measurement protocols. A key finding is that only 16.66% of the reviewed studies utilized the Douglas Bag method, with the majority relying on metabolic carts. While these carts are widely used, their variability raises concerns about cross-device comparability and standardization. Future research should focus on direct comparisons with gold-standard methods to enhance measurement accuracy and reproducibility.
To improve the clinical and research applicability of these devices, standardized validation frameworks should be established. Strengthening the reliability, validity, and accuracy of metabolic measurements will enhance their use across diverse populations and settings, ultimately improving their utility in both clinical practice and scientific research.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/sports13070198/s1, Supplementary S1: The PRISMA-DTA Checklist.

Author Contributions

Conceptualization, C.U.O.-L. and G.R.-U.; methodology, M.A.H.-L. and L.P.-R.; writing—original draft preparation: F.J.O.-A. and I.A.C.-G.; writing—review and editing, H.E.A.-R. and F.J.A.-G.; supervision, M.A.H.-L. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Secretaría de Ciencia, Humanidades, Tecnología e Inno-vación (Secihti), Grant ApoyosLNC-2023-69, which was awarded to M.A.H.-L.

Data Availability Statement

Data are available upon request to the corresponding author (M.A.H.-L.).

Acknowledgments

The LaNCoCoME acknowledge the support received from Secihti in the years 2024 and 2025 and from the Master Degree Scholarship provided for the first author, C.U.O.-L. and from the Postdoctoral Scholarship provided for the second author, F.J.O.-A. The Academic Body “Salud Personalizada (UABC-CA-336)” acknowledges the support received by the “Coordinación General de Investigación y Posgrado”of the Autonomous University of Baja California in the year 2025 for the project registered at the Sistema de Captura y Seguimiento de Proyectos de Investigación (SICASPI), with Code No. 304/865/E.

Conflicts of Interest

The authors declare no conflicts of interest. The funders had no role in the design of the study, in the writing of the manuscript, or in the decision to publish the results, and will not have a role in the collection, analyses, or interpretation of data.

References

  1. O’Neill, J.E.; Corish, C.A.; Horner, K. Accuracy of resting metabolic rate prediction equations in athletes: A systematic review with meta-analysis. Sports Med. 2023, 53, 2373–2398. [Google Scholar] [CrossRef]
  2. Fernández-Verdejo, R.; Sanchez-Delgado, G.; Ravussin, E. Energy Expenditure in Humans: Principles, Methods, and Changes Throughout the Life Course. Annu. Rev. Nutr. 2024, 44, 51–76. [Google Scholar] [CrossRef] [PubMed]
  3. Iraki, J.; Paulsen, G.; Garthe, I.; Slater, G.; Areta, J.L. Reliability of resting metabolic rate between and within day measurements using the Vyntus CPX system and comparison against predictive formulas. Nutr. Health 2023, 29, 107–114. [Google Scholar] [CrossRef] [PubMed]
  4. Müller, M.J.; Geisler, C. From the past to future: From energy expenditure to energy intake to energy expenditure. Eur. J. Clin. Nutr. 2017, 71, 358–364. [Google Scholar] [CrossRef] [PubMed]
  5. Shephard, R. Open-circuit respirometry: A brief historical review of the use of Douglas bags and chemical analyzers. Eur. J. Appl. Physiol. 2017, 117, 381–387. [Google Scholar] [CrossRef]
  6. Redondo, R.B. Resting energy expenditure; assessment methods and applications. Nutr. Hosp. 2015, 31, 245–253. [Google Scholar] [CrossRef]
  7. Delsoglio, M.; Achamrah, N.; Berger, M.M.; Pichard, C. Indirect calorimetry in clinical practice. J. Clin. Med. 2019, 8, 1387. [Google Scholar] [CrossRef]
  8. O’Driscoll, R.; Turicchi, J.; Beaulieu, K.; Scott, S.; Matu, J.; Deighton, K.; Finlayson, G.; Stubbs, J. How well do activity monitors estimate energy expenditure? A systematic review and meta-analysis of the validity of current technologies. Br. J. Sports Med. 2020, 54, 332–340. [Google Scholar] [CrossRef]
  9. Hodges, L.D.; Brodie, D.A.; Bromley, P.D. Validity and reliability of selected commercially available metabolic analyzer systems. Scand. J. Med. Sci. Sports 2005, 15, 271–279. [Google Scholar] [CrossRef]
  10. Heydenreich, J.; Kayser, B.; Schutz, Y.; Melzer, K. Total energy expenditure, energy intake, and body composition in endurance athletes across the training season: A systematic review. Sports Med. Open 2017, 3, 8. [Google Scholar] [CrossRef]
  11. Di Paco, A.; Bonilla, D.A.; Perrotta, R.; Canonico, R.; Cione, E.; Cannataro, R. Validity and reliability of a new wearable chest strap to estimate respiratory frequency in elite soccer athletes. Sports 2024, 12, 277. [Google Scholar] [CrossRef] [PubMed]
  12. Van Hooren, B.; Souren, T.; Bongers, B.C. Accuracy of respiratory gas variables, substrate, and energy use from 15 CPET systems during simulated and human exercise. Scand. J. Med. Sci. Sports 2024, 34, e14490. [Google Scholar] [CrossRef] [PubMed]
  13. McInnes, M.D.; Moher, D.; Thombs, B.D.; McGrath, T.A.; Bossuyt, P.M.; Clifford, T.; Cohen, J.F.; Deeks, J.J.; Gatsonis, C.; Hooft, L. Preferred reporting items for a systematic review and meta-analysis of diagnostic test accuracy studies: The PRISMA-DTA statement. JAMA 2018, 319, 388–396. [Google Scholar] [CrossRef]
  14. National Institutes of Health. Study Quality Assessment Tools. 2021. Available online: https://www.nhlbi.nih.gov/health-topics/study-quality-assessment-tools (accessed on 31 March 2025).
  15. St-Onge, M.; Rubiano, F.; Jones, A.; Heymsfield, S.B. A New Hand-Held Indirect Calorimeter to Measure Postprandial Energy Expenditure. Obes. Res. 2004, 12, 704–709. [Google Scholar] [CrossRef] [PubMed]
  16. Compher, C.; Hise, M.; Sternberg, A.; Kinosian, B.P. Comparison between Medgem and Deltatrac resting metabolic rate measurements. Eur. J. Clin. Nutr. 2005, 59, 1136–1141. [Google Scholar] [CrossRef]
  17. Hlynsky, J.; Birmingham, C.; Johnston, M.; Gritzner, S. The agreement between the MedGem® indirect calorimeter and a standard indirect calorimeter in anorexia nervosa. Eat. Weight. Disord.-Stud. Anorex. Bulim. Obes. 2005, 10, e83–e87. [Google Scholar] [CrossRef]
  18. Stewart, C.L.; Goody, C.M.; Branson, R. Comparison of Two Systems of Measuring Energy Expenditure. J. Parenter. Enter. Nutr. 2005, 29, 212–217. [Google Scholar] [CrossRef]
  19. Frankenfield, D.C.; Coleman, A. An Evaluation of a Handheld Indirect Calorimeter Against a Standard Calorimeter in Obese and Nonobese Adults. J. Parenter. Enter. Nutr. 2013, 37, 652–658. [Google Scholar] [CrossRef]
  20. Cooper, J.A.; Watras, A.C.; O’Brien, M.J.; Luke, A.; Dobratz, J.R.; Earthman, C.P.; Schoeller, D.A. Assessing Validity and Reliability of Resting Metabolic Rate in Six Gas Analysis Systems. J. Am. Diet. Assoc. 2009, 109, 128–132. [Google Scholar] [CrossRef]
  21. Welch, W.; Strath, S.; Swartz, A. Congruent Validity and Reliability of Two Metabolic Systems to Measure Resting Metabolic Rate. Int. J. Sports Med. 2015, 36, 414–418. [Google Scholar] [CrossRef]
  22. Nieman, D.C.; Austin, M.D.; Benezra, L.; Pearce, S.; McInnis, T.; Unick, J.; Gross, S.J. Validation of Cosmed’s FitMateTM in Measuring Oxygen Consumption and Estimating Resting Metabolic Rate. Res. Sports Med. 2006, 14, 89–96. [Google Scholar] [CrossRef] [PubMed]
  23. Vandarakis, D.; Salacinski, A.J.; Broeder, C.E. A Comparison of Cosmed Metabolic Systems for the Determination of Resting Metabolic Rate. Res. Sports Med. 2013, 21, 187–194. [Google Scholar] [CrossRef]
  24. Purcell, S.A.; Johnson-Stoklossa, C.; Braga Tibaes, J.R.; Frankish, A.; Elliott, S.A.; Padwal, R.; Prado, C.M. Accuracy and reliability of a portable indirect calorimeter compared to whole-body indirect calorimetry for measuring resting energy expenditure. Clin. Nutr. ESPEN 2020, 39, 67–73. [Google Scholar] [CrossRef] [PubMed]
  25. Oshima, T.; Delsoglio, M.; Dupertuis, Y.M.; Singer, P.; De Waele, E.; Veraar, C.; Heidegger, C.P.; Wernermann, J.; Wischmeyer, P.E.; Berger, M.M.; et al. The clinical evaluation of the new indirect calorimeter developed by the ICALIC project. Clin. Nutr. 2020, 39, 3105–3111. [Google Scholar] [CrossRef]
  26. Dupertuis, Y.M.; Delsoglio, M.; Hamilton-James, K.; Berger, M.M.; Pichard, C.; Collet, T.H.; Genton, L. Clinical evaluation of the new indirect calorimeter in canopy and face mask mode for energy expenditure measurement in spontaneously breathing patients. Clin. Nutr. 2022, 41, 1591–1599. [Google Scholar] [CrossRef]
  27. Alcantara, J.M.A.; Galgani, J.E.; Jurado-Fasoli, L.; Dote-Montero, M.; Merchan-Ramirez, E.; Ravussin, E.; Ruiz, J.R.; Sanchez-Delgado, G. Validity of four commercially available metabolic carts for assessing resting metabolic rate and respiratory exchange ratio in non-ventilated humans. Clin. Nutr. 2022, 41, 746–754. [Google Scholar] [CrossRef] [PubMed]
  28. Alcantara, J.M.A.; Sanchez-Delgado, G.; Martinez-Tellez, B.; Merchan-Ramirez, E.; Labayen, I.; Ruiz, J.R. Congruent validity and inter-day reliability of two breath by breath metabolic carts to measure resting metabolic rate in young adults. Nutr. Metab. Cardiovasc. Dis. 2018, 28, 929–936. [Google Scholar] [CrossRef]
  29. Wang, X.; Wang, Y.; Ding, Z.; Cao, G.; Hu, F.; Sun, Y.; Ma, Z.; Zhou, D.; Su, B. Relative validity of an indirect calorimetry device for measuring resting energy expenditure and respiratory quotient. Asia Pac. J. Clin. Nutr. 2018, 27, 72–77. [Google Scholar] [CrossRef]
  30. Poulsen, M.K.; Thomsen, L.P.; Kjærgaard, S.; Rees, S.E.; Karbing, D.S. Reliability of, and Agreement Between, two Breath-by-Breath Indirect Calorimeters at Varying Levels of Inspiratory Oxygen. Nutr. Clin. Pract. 2019, 34, 767–774. [Google Scholar] [CrossRef]
  31. Graf, S.; Karsegard, V.; Viatte, V.; Maisonneuve, N.; Pichard, C.; Genton, L. Comparison of three indirect calorimetry devices and three methods of gas collection: A prospective observational study. Clin. Nutr. 2013, 32, 1067–1072. [Google Scholar] [CrossRef]
  32. Zhao, D.; Xian, X.; Terrera, M.; Krishnan, R.; Miller, D.; Bridgeman, D. A pocket-sized metabolic analyzer for assessment of resting energy expenditure. Clin. Nutr. 2014, 33, 341–347. [Google Scholar] [CrossRef] [PubMed]
  33. Rosdahl, H.; Gullstrand, L.; Salier-Eriksson, J.; Johansson, P.; Schantz, P. Evaluation of the Oxycon Mobile metabolic system against the Douglas bag method. Eur. J. Appl. Physiol. 2010, 109, 159–171. [Google Scholar] [CrossRef]
  34. Medbø, J.I.; Mamen, A.; Resaland, G.K. New examination of the performance of the MetaMax I metabolic analyser with the Douglas-bag technique. Scand. J. Clin. Lab. Investig. 2012, 72, 158–168. [Google Scholar] [CrossRef]
  35. Astrup, A.; Gøtzsche, P.; Van de Werken, K.; Ranneries, C.; Toubro, S.; Raben, A.; Buemann, B. Meta-analysis of resting metabolic rate in formerly obese subjects. Am. J. Clin. Nutr. 1999, 69, 1117–1122. [Google Scholar] [CrossRef] [PubMed]
  36. Khalaj-Hedayati, K.; Bosy-Westphal, A.; Müller, M.; Dittmar, M. Validation of the BIOPAC indirect calorimeter for determining resting energy expenditure in healthy free-living older people. Nutr. Res. 2009, 29, 531–541. [Google Scholar] [CrossRef]
  37. Overstreet, B.; Bassett, D., Jr.; Crouter, S.; Rider, B.; Parr, B. Portable open-circuit spirometry systems. J. Sport Med. Phys. Fit. 2016, 57, 227–237. [Google Scholar] [CrossRef]
  38. Macfarlane, D. Open-circuit respirometry: A historical review of portable gas analysis systems. Eur. J. Appl. Physol. 2017, 117, 2369–2386. [Google Scholar] [CrossRef] [PubMed]
  39. Robles-González, L.; Gutiérrez-Hellín, J.; Aguilar-Navarro, M.; Ruiz-Moreno, C.; Muñoz, A.; Del-Coso, J.; Ruiz, J.R.; Amaro-Gahete, F.J. Inter-Day Reliability of Resting Metabolic Rate and Maximal Fat Oxidation during Exercise in Healthy Men Using the Ergostik Gas Analyzer. Nutrients 2021, 13, 4308. [Google Scholar] [CrossRef]
  40. Hopkins, W.; Marshall, S.; Batterham, A.; Hanin, J. Progressive statistics for studies in sports medicine and exercise science. Med. Sci. Sports Exerc. 2009, 41, 3. [Google Scholar] [CrossRef]
Sports 13 00198 g001
Figure 1. PRISMA flow diagram for the search strategy employed and results obtained.
Figure 1. PRISMA flow diagram for the search strategy employed and results obtained.
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Table 1. Characteristics of the studies evaluating validity of resting metabolic rate using different portable gas analyzers.
Table 1. Characteristics of the studies evaluating validity of resting metabolic rate using different portable gas analyzers.
Reference Tested DeviceReference DevicePopulation VariablesProtocolAccuracyReliabilityConclusion
St-Onge et al., (2004) [15]MedGem Delta-Trac15 (F = 6, M = 9), 36 ± 3.4 yearsRMRRMR measured for 20 min with Delta- Trac, 10 min with MedGem. Same day, random order.No difference in RMR (6455.1 ± 417.6 vs. 6468.5 ± 337.2 kJ/d) between Delta-Trac and MedGem, respectivelyNot availableMedGem is accurate for RMR comparable to Delta-Trac
Compher et al., (2005) [16] MedGemDelta-Trac24 (F = 13, M = 11), 46.8 ± 15.1 yearsRMR, VO2RMR measurements for 20 min. Same day, random order.Difference in RMR (1297.67 ± 202.1 vs. 1445.77 ± 285.7 kcal/day) between Delta-Trac and MedGem, respectivelyNo difference for reproducibility (1301.97 ± 180.9 vs. 1295.77 ± 223.4 kcal/day) Mean difference betwen measures of 6.8 kcal, with limits of agreement from 233 to 247 kcalMedGem has adequate reproducibility, but its clinical use should be carefully considered, especially for vulnerable populations requiring precise measurements
Hlynsky et al., (2005) [17] MedGem Delta-TracF = 27, n = 12 subjets with anorexia, n = 15 control group (32 ± 8 years)RMR, VO2RMR measured using MedGem (10 min) and DeltaTrac (20 min). Same day.Mean difference of 123.3 ± 264.5 kcal/day between Deltatrac and the MedGem. Correlations of RMR (r = 0.60, p = 0.04) for subjects with anorexia and (r = 0.04, p = 0.89) for control groupNot availableMedGem did not provide a reliable measure of RMR when compared with the Delta-Trac
Stewart et al., (2005) [18]MedGem Delta-Trac50 (F = 38, M = 12) 33.8 ± 13.2 yearsRMR, VO2RMR measured for 10 min in a reclined position. Same day.Mean difference for RMR mean (4.66 ± 113.39 kcal/day) (p = 0.773) and the correlation coefficient for RMR was r = 0.941 p ˂ 0.01)Not availableMedGem measures oxygen consumption and RMR
accurately where traditional metabolic carts are impractical or costly
Frankenfield & Coleman, (2013) [19]MedGem Delta-Trac100 (F = 84, M = 16) 44 ± 15 yearsRMR, VO2RMR measured in a reclined and supine position for 10 min (Delta-Trac) and in seated position for MedGem. Same day, random order.Difference between MedGem and Delta-Trac measurement in semi recumbent posture. Oxygen consumption (273 ± 58 vs. 247 ± 44 mL/min and No significant bias in the non-obesity groupRMR absolute
difference was 61 ± 49 kcal/day. A total of 73% of the repeated measures had a
95% CI: (55–86%)		MedGem can be useful, but its accuracy varies based on obesity status, showing bias in obese individuals
Cooper et al., (2009) [20]MedGem, Parvomedics TrueOne 2400, MedGraphics CPX Ultima, Korr ReeVue, Vmax Encore SystemDelta-Trac41 (F = 34, M = 7), 49 ± 9 yearsRMRRMR measured for 30 min on each device, excluding first and last 5 min. different day, random order.All of the RMR CVs (Ultima 10.9%, Korr 11.9%, Vmax 8% and True One 4.8%) was significantly larger than the CV for the Delta-Trac (3%) Not availableTrueOne and the Vmax were the most valid gas analysis systems of those tested for measuring both RMR relative to the Delta-Trac; Variability in RMR measurement consistency suggests that the choice of gas analysis system can influence results
Welch et al., (2015) [21]ParvoMedics TrueOne 2400Cosmed K4b231 (F = 13, M = 18), 27.3 ± 7.8 yearsRMR, FeO2, VO2Supine RMR measurement, 10 min rest, results averaged per minute. Different day, random order.No significant difference in RMR (kcal/day). Difference in FEO2 (Parvo2: 19.68%, Cosmed K4b2: 16.63%), Significant difference in measured kcal/day (p = 0.036) between all Cosmed RMR measurements, mean difference between Cosmed2−Cosmed1 (135.0 ± 334.7) and Cos-
med2−Cosmed3 (−43.2 ± 352.7).		Due to differences in measurement technology, FEO2 was significantly different between systems, but the resultant RMR values were not significantly different
Nieman et al., (2006) [22]FitMate Douglas Bag (capacity not especified)60 (F = 30, M = 30), M: 37.9 ± 13.4, F:139.8 ± 12.9 yearsRMR, VO2RMR measured for 10 min on both devices. Same day.No differences between Douglas Bag and FitMate for VO2 (242 ± 49 mL/min vs. 240 ± 49 mL/min, p = 0.066) and RMR (1662 ± 340 kcal/day vs. 1668 ± 344 kcal/day, p = 0.579). Absolute difference 5.81 ± 80.70 kcal/day)Not availableFitMate is a reliable and valid system for measuring VO2 and RMR in adults, showing high consistency with the reference method
Vandarakis et al., (2013) [23]FitMateQuark CPET30 (F = 15, M = 15), 28.4 ± 7 yearsRMR, VO2RMR measured twice for 10 min on each device in a supine positionNo differences between Quark CPET and FitMate for measured variables VO2 (r-value = 0.98, p = 0.0001), RMR (r-value = 0.96, p = 0.0001). RMR values between systems were 0.83%, mean difference of 5.95 kcal/day.Not availableFitMate is reliable for measuring RMR in healthy adults
Purcell et al., (2020) [24]Fitmate GS Whole Body Calorimetry77 (F = 49, M = 28), 32 ± 8 yearsRMR, VO2RMR measured using Fitmate GS (10 min) and WBC (30 min). Different days, random order.Fitmate GS showed significantly higher VO2 (229 [IQR: 197–272] vs. 263 [IQR: 229–301] mL/min, p < 0.001). Fitmate GS underestimated RMR (1680 ± 420 vs. 1916 ± 461 kcal/day, p < 0.001)RMR with Fitmate GS was of ICC 0.80 (95% CI: 0.70–0.87). Mean differences −28 kcal/day (normal or overweight) to 14 kcal/day (obesity).Fitmate GS has discrepancies compared to whole-body calorimetry, affecting its accuracy and precision. No significant relationship between bias and body composition variables
Oshima et al., (2020) [25] Cosmed Q-NRGDelta-Trac, Quark RMR, V-max, ECOVX277 (F = 128, M = 149), 67 ± 13RMRRMR measured for 20–30 min on all devices. Same day.RMR differences between Cosmed Q-NRG (307.4 ± 324.5, p < 0.001), Quark RMR (224.4 ± 514.9, p = 0.038), and V-max (449.6 ± 667.4, p < 0.001) vs. Delta-TracNot availableCosmed Q-NRG is effective and consistent for RMR measurement compared to currently used devices
Dupertuis et al., (2022) [26] Cosmed Q-NRG Quark RMR85 (F = 45, M = 40), 53 ± 18 yearsRMR, VO2Rest time: 10–20 min, Measurement: 15 min. Same day, random order.Higher correlation when Cosmed Q-NRG was used in canopy hood than in face mask mode (r = 0.96 and 0.86). Face mask mode overestimated RMR by 150 ± 51 kcal/day compared to canopy hood modeNot availableHood mode of Q-NRG is more suitable for lower-weight patients, providing precise and consistent VO2 measurements. Mask mode may present stability and accuracy challenges
Alcantara et al., (2022) [27]Cosmed Q-NRG, Vyaire Vyntus CPX, Omnical Medgraphics, Ultima CardiO2Comparison between the four gas analyzers29, F = 11, M = 18, 24 ± 4 yearsRMR, VO2RMR measured for 30 min on both devices. Different days, random order.Measurement error for RMR (Omnical = 1.5 ± 0.5%; Q- NRG = 2.5 ± 1.3%; Ultima = 10.7 ± 11.0%; Vyntu s= 13.8 ± 5.0%)No differences (p = 0.058) for RMR within-subject reproducibility (inter-day CV: Q-NRG = 3.6 ± 2.5%; Omnical = 4.8 ± 3.5%; Vyntus = 5.0 ± 5.6%; Ultima = 5.7 ± 4.6%), There is variability between devices; the Omnical device appears to be the most suitable for measuring RMR and RER
Alcantara et al., (2018) [28] CCM Express Ultima CardiO2 (MGU)17 (F = 11, M = 6), 23.2 ± 2.7 yearsRMR, VO2RMR measured for 20 min on both devices. Different days, random order.Mean difference between devices for RMR 65 ± 161 Kcal/dayAbsolute inter-day RMR differences (158 ± 154 vs. 219 ± 185 kcal/day) or (18.3 ± 17.2 vs. 13.5 ± 15.3) between MGU and CCM.Both devices are consistent in RMR measurement but show significant differences in their absolute values. CCM its more reliable
Wang et al., (2018) [29] IIM-IC-100VO2000 Medical Graphics Corp32, F = 17, M = 15, 25 ± 6 yearsRMR, VO2 Measurement in supine position for 15 min. for both teams. Same day random orderMean difference between devices for RMR 81.3 kcal/d (5.83%). The CV were 5.9% and 10.3% for VO2; 5.8% and 10.5% for RMR Significant correlations between repeated measurements for both the IIM-IC-100 (VO2: r = 0.95, VCO2: r = 0.91, REE: r = 0.95; p < 0.001) and VO2000 (VO2: r = 0.90, VCO2: r = 0.85, REE: r = 0.90; p < 0.001). The IIM-IC-100 showed high consistency and accuracy in the measurement of RMR and RQ, comparable to the VO2000
Poulsen et al., (2019) [30] Beacon 3Ecovx F-CM1-0416 M, 33 ± 9 yearsRMR, VO2, FiO2Four consecutive periods of 15 min in sitting position. at different FiO2 levels: 21%, 50%, 85%, and again 21%. Same day, random order.Differences in RMR and VO2 between devices at differents levels of FiO2, especially at 85% (9%) (p = 0.000 for VO2 and p = 0.001 for RMR) The CVs for EE at 21% FiO2 was
Beacon 3 (4.8%) and Ecovx (4.0%)		Although both devices can be used to measure energy expenditure, differences in their results should be considered, especially in high FiO2 conditions, which could affect the clinical interpretation of the data obtained
Graf et al., (2013) [31] QuarkRMR CCMexpressDelta-Trac24 (F = 15, M = 9), 53 ± 15 yearsRMR, VO2Rest time: 15 min, Measurement 10 min. Same day.Mean RMR measured by CCMexpress canopy was (7%) higher than Delta-Trac (p = 0.004) The RMR limits of agreement were high (±402 kcal for CCMexpress (facemask), and ±304 kcal for CCMexpress (face tent)Not availableMean RMR measured by QuarkRMR is similar to Delta-Trac but the limits of agreement are high. Mean RMR measured by CCMexpress (canopy) was overestimated compared to Delta-Trac. None of the compared devices ideally replaces the Delta-Trac measurements
Zhao et al., (2014) [32] Pocket-Sized Metabolic Analyzer Douglas Bag (4-L)30 (F = 15, M = 15), 27 ± 6 yearsRMR, VO2Collection of 4 L of exhaled oxygen while seated to calculate RMRSignificant correlation and agreement for RMR and VO2 between devices. Differences averaged 10%. Difference between devices for RMR 3.2%Not availableThe Pocket-Sized Metabolic Analyzer shows high accuracy for measuring RMR and VO2 compared to the Douglas bag
CV: coefficient of variation; F: female; FeO2: fraction of oxygen in exhaled air; FiO2: fraction of inspired oxygen; M: male; RMR: resting metabolic rate; VO2: oxygen consumption.
Table 2. Methodological quality assessment tool for observational cohort and cross-sectional studies.
Table 2. Methodological quality assessment tool for observational cohort and cross-sectional studies.
Reference Q1Q2Q3Q4Q5Q6Q7Q8Q9Q10Q11Q12Q13Q14Score%Risk of Bias
St-Onge et al. [15] YesYesNDYesNoYesYesNoYesNoYesYesYesYes10/1471.4Regular
Compher et al. [16] YesYesNDYesNoYesYesNoYesNoYesYesYesYes10/1471.4Regular
Hlynsky et al. [17] YesYesYesYesNoYesYesYesYesNoYesYesYesYes12/1485.7Low
Stewart et al. [18] YesYesNDYesNoYesYesYesYesNoYesYesNRYes10/1471.4Regular
Frankenfield & Coleman, [19] YesYesNDYesYesYesYesNoYesNoYesYesYesYes11/1478.6Low
Cooper et al. [20] YesYesNDYesNoYesYesNoYesNoYesYesYesYes10/1471.4Regular
Welch et al. [21] YesYesNDYesNoYesYesNoYesNoYesYesNRYes9/1464.3Regular
Nieman et al. [22] YesYesNDYesNoYesYesNoYesYesYesYesYesYes11/1478.6Low
Vandarakis et al. [23] YesYesNDYesNoYesYesNoYesNoYesYesYesYes10/1471.4Regular
Purcell et al. [24] YesYesNDYesNoYesYesNoYesNoYesYesNRYes9/1464.3Regular
Oshima et al. [25] YesYesNDYesNoYesYesNoYesNoYesYesYesYes10/1471.4Regular
Dupertuis et al. [26] YesYesNDYesNoYesYesNoYesNoYesYesNRYes9/1464.3Regular
Alcantara et al. [27] YesYesNDYesNoYesYesNoYesNoYesYesYesYes10/1471.4Regular
Alcantara et al. [28] YesYesNDYesNoYesYesNoYesNoYesYesYesYes10/1471.4Regular
Wang et al. [29] YesYesNDYesNoYesYesYesYesYesYesYesYesYes12/1485.7Low
Poulsen et al. [30] YesYesNDYesYesYesYesYesYesNoYesYesYesYes12/1485.7Low
Graf et al. [31] YesYesNDYesNoYesYesYesYesYesYesYesYesYes12/1485.7Low
Zhao et al. [32] YesYesYesYesYesYesYesNoYesNoYesYesYesYes12/1485.7Low
ND = not determinable; NR = not reported. Q1. Was the research question or objective in this paper clearly stated?; Q2. Was the study population clearly specified and defined?; Q3. Was the participation rate of eligible persons at least 50%?; Q4. Were all the subjects selected or recruited from the same or similar populations (including the same time period)? Were inclusion and exclusion criteria for being in the study prespecified and applied uniformly to all participants?; Q5. Was a sample size justification, power description, or variance and effect estimates provided?; Q6. For the analyses in this paper, were the exposure(s) of interest measured prior to the outcome(s) being measured?; Q7. Was the timeframe sufficient so that one could reasonably expect to see an association between exposure and outcome if it existed?; Q8. For exposures that can vary in amount or level, did the study examine different levels of the exposure as related to the outcome?; Q9. Were the exposure measures (independent variables) clearly defined, valid, reliable, and implemented consistently across all study participants?; Q10. Was/were the exposure(s) assessed more than once over time?; Q11. Were the outcome measures (dependent variables) clearly defined, valid, reliable, and implemented consistently across all study participants?; Q12. Were the outcome assessors blinded to the exposure status of participants?; Q13. Was loss to follow-up after baseline 20% or less?; Q14. Were key potential confounding variables measured and adjusted statistically for their impact on the relationship between exposure(s) and outcome(s)? Background colors are presented to show a yes (green) or no (orange) answer to each item.
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Olivas-León, C.U.; Olivas-Aguirre, F.J.; Chávez-Guevara, I.A.; Almanza-Reyes, H.E.; Patrón-Romero, L.; Rodríguez-Uribe, G.; Amaro-Gahete, F.J.; Hernández-Lepe, M.A. Using Respiratory Gas Analyzers to Determine Resting Metabolic Rate in Adults: A Systematic Review of Validity Studies. Sports 2025, 13, 198. https://doi.org/10.3390/sports13070198

AMA Style

Olivas-León CU, Olivas-Aguirre FJ, Chávez-Guevara IA, Almanza-Reyes HE, Patrón-Romero L, Rodríguez-Uribe G, Amaro-Gahete FJ, Hernández-Lepe MA. Using Respiratory Gas Analyzers to Determine Resting Metabolic Rate in Adults: A Systematic Review of Validity Studies. Sports. 2025; 13(7):198. https://doi.org/10.3390/sports13070198

Chicago/Turabian Style

Olivas-León, César Ulises, Francisco Javier Olivas-Aguirre, Isaac Armando Chávez-Guevara, Horacio Eusebio Almanza-Reyes, Leslie Patrón-Romero, Genaro Rodríguez-Uribe, Francisco José Amaro-Gahete, and Marco Antonio Hernández-Lepe. 2025. "Using Respiratory Gas Analyzers to Determine Resting Metabolic Rate in Adults: A Systematic Review of Validity Studies" Sports 13, no. 7: 198. https://doi.org/10.3390/sports13070198

APA Style

Olivas-León, C. U., Olivas-Aguirre, F. J., Chávez-Guevara, I. A., Almanza-Reyes, H. E., Patrón-Romero, L., Rodríguez-Uribe, G., Amaro-Gahete, F. J., & Hernández-Lepe, M. A. (2025). Using Respiratory Gas Analyzers to Determine Resting Metabolic Rate in Adults: A Systematic Review of Validity Studies. Sports, 13(7), 198. https://doi.org/10.3390/sports13070198

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