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Implication of m6A mRNA Methylation in Susceptibility to Inflammatory Bowel Disease

1
Department of Genetics, Physical Anthropology and Animal Fisiology, University of the Basque Country, 48940 Leioa, Spain
2
Biocruces Bizkaia Health Research Institute, 48903 Barakaldo, Spain
3
Department of Biochemistry and Molecular Biology, University of the Basque Country, 48940 Leioa, Spain
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CIBER (Centro de Investigación Biomédica en Red) de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, Spain
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Hepatic and Gastrointestinal Disease Area, IIS Biodonostia, 20014 Donostia, Spain
6
Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain
*
Author to whom correspondence should be addressed.
Epigenomes 2020, 4(3), 16; https://doi.org/10.3390/epigenomes4030016
Received: 29 June 2020 / Revised: 21 July 2020 / Accepted: 28 July 2020 / Published: 3 August 2020
Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract that develops due to the interaction between genetic and environmental factors. More than 160 loci have been associated with IBD, but the functional implication of many of the associated genes remains unclear. N6-Methyladenosine (m6A) is the most abundant internal modification in mRNA. m6A methylation regulates many aspects of mRNA metabolism, playing important roles in the development of several pathologies. Interestingly, SNPs located near or within m6A motifs have been proposed as possible contributors to disease pathogenesis. We hypothesized that certain IBD-associated SNPs could regulate the function of genes involved in IBD development via m6A-dependent mechanisms. We used online available GWAS, m6A and transcriptome data to find differentially expressed genes that harbored m6A-SNPs associated with IBD. Our analysis resulted in five candidate genes corresponding to two of the major IBD subtypes: UBE2L3 and SLC22A4 for Crohn’s Disease and TCF19, C6orf47 and SNAPC4 for Ulcerative Colitis. Further analysis using in silico predictions and co-expression analyses in combination with in vitro functional studies showed that our candidate genes seem to be regulated by m6A-dependent mechanisms. These findings provide the first indication of the implication of RNA methylation events in IBD pathogenesis. View Full-Text
Keywords: inflammatory bowel disease; Crohn’s disease; ulcerative colitis; m6A; SNP; METTL3; YTHDF1; inflammation inflammatory bowel disease; Crohn’s disease; ulcerative colitis; m6A; SNP; METTL3; YTHDF1; inflammation
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MDPI and ACS Style

Sebastian-delaCruz, M.; Olazagoitia-Garmendia, A.; Gonzalez-Moro, I.; Santin, I.; Garcia-Etxebarria, K.; Castellanos-Rubio, A. Implication of m6A mRNA Methylation in Susceptibility to Inflammatory Bowel Disease. Epigenomes 2020, 4, 16. https://doi.org/10.3390/epigenomes4030016

AMA Style

Sebastian-delaCruz M, Olazagoitia-Garmendia A, Gonzalez-Moro I, Santin I, Garcia-Etxebarria K, Castellanos-Rubio A. Implication of m6A mRNA Methylation in Susceptibility to Inflammatory Bowel Disease. Epigenomes. 2020; 4(3):16. https://doi.org/10.3390/epigenomes4030016

Chicago/Turabian Style

Sebastian-delaCruz, Maialen; Olazagoitia-Garmendia, Ane; Gonzalez-Moro, Itziar; Santin, Izortze; Garcia-Etxebarria, Koldo; Castellanos-Rubio, Ainara. 2020. "Implication of m6A mRNA Methylation in Susceptibility to Inflammatory Bowel Disease" Epigenomes 4, no. 3: 16. https://doi.org/10.3390/epigenomes4030016

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