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Epigenomes 2018, 2(1), 5;

Panobinostat Potentiates Temozolomide Effects and Reverses Epithelial–Mesenchymal Transition in Glioblastoma Cells

Department of Biochemistry and Genetics, University of Navarra School of Sciences, 31008 Pamplona, Spain
Molecular Pathology Research Unit, Virgen de la Salud Hospital, 45005 Toledo, Spain
IdiPaz Research Unit, La Paz University Hospital, 28046 Madrid, Spain
Department of Pathology, University of Navarra Clinic, 31008 Pamplona, Spain
Author to whom correspondence should be addressed.
Received: 30 December 2017 / Revised: 6 February 2018 / Accepted: 13 February 2018 / Published: 19 February 2018
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Glioblastoma is the most common form of glioma, as well as the most aggressive. Patients suffering from this disease have a very poor prognosis. Surgery, radiotherapy, and temozolomide are the only approved treatments nowadays. Panobinostat is a pan-inhibitor of histone deacetylases (HDACs) that has been shown to break some pathways which play an important role in cancer development. A global intention of using panobinostat as a therapeutic agent against glioblastoma is beginning to be a reality. We have treated the LN405 glioblastoma cell line with temozolomide, panobinostat, and combined treatment, in order to test apoptosis, colony formation, and a possible molecular reversion of the mesenchymal phenotype of the cells to an epithelial one. Our results show that panobinostat decreased N-cadherin levels in the LN405 glioblastoma cell line while it increased the expression of E-cadherin, which might be associated with a mesenchymal–epithelial transition in glioblastoma cells. Colony formation was reduced, and apoptosis was increased with treatments. Our research highlights the importance of panobinostat as a potential adjuvant therapy to be used with temozolomide to treat glioblastoma and the advantages of the combined treatment versus temozolomide alone, which is currently the first-line treatment used to treat this tumor. View Full-Text
Keywords: panobinostat; temozolomide; glioblastoma; epithelial-mesenchymal transition panobinostat; temozolomide; glioblastoma; epithelial-mesenchymal transition

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Urdiciain, A.; Meléndez, B.; Rey, J.A.; Idoate, M.A.; Castresana, J.S. Panobinostat Potentiates Temozolomide Effects and Reverses Epithelial–Mesenchymal Transition in Glioblastoma Cells. Epigenomes 2018, 2, 5.

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