Increase of DNA Methylation at the HvCKX2.1 Promoter by Terminal Drought Stress in Barley
Abstract
:1. Introduction
2. Results
2.1. Identification of Terminal Drought Stress Specific Small RNAs in Barley Caryopses
2.2. Methylation Status of ProHvCKX2.1
2.3. Seedlings Derived from Drought Stress Plants Exhibit Reduced Presence of HvCKX2.1 mRNA
2.4. Terminal Drought Stress Enhances the Shoot Emergence Rate of Seeds
2.5. Barley with Increased Levels of ABA During Grain Development Phase Show Faster Germination
3. Discussion
4. Material and Methods
4.1. Plant Cultivation and Used Barley Plants
4.2. Small RNA Sequencing
- “hairpin_and_mature_Hordeum_vulgare.fa”from miRBASE
- “hairpin_and_mature.fa”from miRBASE
- “nt.viridiplantae.fas”
4.3. Transcript Quantification
4.4. DNA Methylation Analysis
4.5. Plant Hormone (PH) Analysis -Abscisic Acid, Cytokinin, Auxin and Salicylic Acid Extraction
Accession Numbers
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Surdonja, K.; Eggert, K.; Hajirezaei, M.-R.; Harshavardhan, V.T.; Seiler, C.; Von Wirén, N.; Sreenivasulu, N.; Kuhlmann, M. Increase of DNA Methylation at the HvCKX2.1 Promoter by Terminal Drought Stress in Barley. Epigenomes 2017, 1, 9. https://doi.org/10.3390/epigenomes1020009
Surdonja K, Eggert K, Hajirezaei M-R, Harshavardhan VT, Seiler C, Von Wirén N, Sreenivasulu N, Kuhlmann M. Increase of DNA Methylation at the HvCKX2.1 Promoter by Terminal Drought Stress in Barley. Epigenomes. 2017; 1(2):9. https://doi.org/10.3390/epigenomes1020009
Chicago/Turabian StyleSurdonja, Korana, Kai Eggert, Mohammad-Reza Hajirezaei, Vokkaliga Thammegowda Harshavardhan, Christiane Seiler, Nicolaus Von Wirén, Nese Sreenivasulu, and Markus Kuhlmann. 2017. "Increase of DNA Methylation at the HvCKX2.1 Promoter by Terminal Drought Stress in Barley" Epigenomes 1, no. 2: 9. https://doi.org/10.3390/epigenomes1020009