Next Article in Journal
Chemical and Non-Chemical Options for Managing Twospotted Spider Mite, Western Tarnished Plant Bug and Other Arthropod Pests in Strawberries
Next Article in Special Issue
Effects of the Environmental Temperature on Aedes aegypti and Aedes albopictus Mosquitoes: A Review
Previous Article in Journal
Molecular Phylogeny, Diversity and Zoogeography of Net-Winged Beetles (Coleoptera: Lycidae)
Previous Article in Special Issue
Odor-Specific Daily Rhythms in the Olfactory Sensitivity and Behavior of Aedes aegypti Mosquitoes
Article Menu

Export Article

Open AccessArticle

Fatty Acid and Related Potassium Kv2 Channel Blockers: Toxicity and Physiological Actions on Mosquitoes

1
Neurotoxicology Laboratory, Department of Entomology and Nematology, Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA
2
USDA, ARS, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL 32608, USA
*
Authors to whom correspondence should be addressed.
Insects 2018, 9(4), 155; https://doi.org/10.3390/insects9040155
Received: 27 September 2018 / Revised: 19 October 2018 / Accepted: 30 October 2018 / Published: 1 November 2018
(This article belongs to the Special Issue Integrative Mosquito Biology: From Molecules to Ecosystems)
  |  
PDF [1215 KB, uploaded 1 November 2018]
  |  

Abstract

Potassium channels constitute a very diverse group involved in neural signaling, neuronal activity, membrane potential maintenance, and action potential generation. Here, we tested the mammalian potassium channel blockers TRAM-34 and 5-hydroxydecanoate (5-HDC), as well as certain fatty acids (FA) that might fit in the lumen of the pore and block channel activity by obstructing K+ ion passage. Kv channel blockers could be leads for a novel pesticide type. Insecticidal activity was assessed by topical application to Anopheles gambiae adult mosquitoes, paralysis in a headless larval assay, at the cellular level with patch-clamp recordings of engineered HEK cells expressing AgKv2.1 channels, as well as central nervous system recordings from larval Drosophila melanogaster. With only one hydroxyl group difference, decanoic acid had a consistently greater effect than 5-HDC in blocking Kv channels, paralyzing larvae, and killing mosquitoes. The 11-dansylamino undecanoic acid (DAUDA) blockage of eukaryotic Kv channels is demonstrated for the first time, but it failed to kill adult mosquitoes. We synthesized alkyl esters from DAUDA and decanoic acid in an effort to improve cuticular penetration, but it had little impact upon adult toxicity. TRAM-34 and rolipram did not show activity on Kv channels nor potent insecticidal effect on adult mosquitoes. Furthermore, co-application of test compounds with permethrin did not increase mortality in adults. In conclusion, the compounds tested had modest insecticidal and synergistic activity. View Full-Text
Keywords: fatty acids; potassium channel; patch-clamp; toxicity; Anopheles gambiae fatty acids; potassium channel; patch-clamp; toxicity; Anopheles gambiae
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Démares, F.; Coquerel, Q.; Richoux, G.; Linthicum, K.; Bloomquist, J. Fatty Acid and Related Potassium Kv2 Channel Blockers: Toxicity and Physiological Actions on Mosquitoes. Insects 2018, 9, 155.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Insects EISSN 2075-4450 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top