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Article

Effects of Yeast on the Growth and Development of Drosophila melanogaster and Pardosa pseudoannulata (Araneae: Lycsidae) Through the Food Chain

by
Yaqi Peng
1,†,
Rui Liu
2,†,
Wei Li
1,
Yao Zhao
3,* and
Yu Peng
1,*
1
Hubei Key Laboratory of Regional Development and Environmental Response, Faculty of Resources and Environmental Science, Hubei University, Wuhan 430062, China
2
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
3
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Insects 2025, 16(8), 795; https://doi.org/10.3390/insects16080795
Submission received: 22 May 2025 / Revised: 26 June 2025 / Accepted: 30 July 2025 / Published: 31 July 2025
(This article belongs to the Section Other Arthropods and General Topics)
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Simple Summary

Pardosa pseudoannulata is a natural predator that helps control insect pests. Since yeast is known to improve the growth and reproduction of Drosophila melanogaster, we tested whether yeast in the flies’ diet affects both the flies and the spiders that eat them. We compared three diets for the flies: no yeast, active yeast, or inactivated yeast. Adding yeast helped fruit flies grow faster, increased their body weight, and boosted their fat, protein, and glucose levels. When spiders ate these yeast-fed flies, their early development (second instar) was faster, but yeast had no effect on their final size or weight. Yeast also increased the spiders’ fat content but did not change their protein or glucose levels. Our findings highlight how yeast in fruit-fly diets influences both flies and their spider predators.

Abstract

Pardosa pseudoannulata plays an important role in the biological control of insect pests. The inclusion of yeast in the culture medium is very important for the growth, development, and reproduction of Drosophila melanogaster, but there have been few studies on the influence of nutrients in the culture medium on spider development. In order to explore the effects of different yeast treatments on the growth and development of D. melanogaster and as a predator, P.  pseudoannulata, three treatments (no yeast, active yeast added, and inactivated yeast added) were adopted to modify the conventional D. melanogaster culture medium. The addition of yeast to the medium shortened the development time from larva to pupation in D. melanogaster. The emergence and larval developmental times of D. melanogaster reared with activated yeast were shorter than those of the group without yeast addition, which promoted D. melanogaster emergence and increased body weight. The addition of yeast to the medium increased the fat, protein, and glucose content in D. melanogaster. The addition of activated yeast shortened the developmental time of P.  pseudoannulata at the second instar stage but had no effect on other instars. Different yeast treat-ments in the medium had no effect on the body length or body weight of P.  pseudoannulata. Adding yeast to D. melanogaster culture medium can increase the total fat content in P.  pseudoannulata, but it has no effect on glucose and total protein in P.  pseudoannulata. Our study shows the importance of yeast to the growth and development of fruit flies.

1. Introduction

Spiders are predators and the most prominent generalist predators in rice fields [1]. They play a key role in controlling rice-field pests [2,3], Although spider nutrition has been studied by many researchers, the effect of the presence of yeast in fruit-fly culture on spiders through the food chain has been poorly studied [4,5,6,7,8]. Studying the nutritional ecology of spiders is critical because it can aid in understanding the evolution of prey capture and life history strategies, factors that regulate both abundance and diversity [9,10]. The nutrient composition of the prey’s diet affects the growth and survivorship of a generalist predator [11,12,13]. Food resources are essential for all aspects of an animal’s life, and the amount and types of food consumed can have an enormous influence on growth, reproduction, and survival [14,15,16,17]. Pardosa pseudoannulata preys mainly on planthoppers, leaf rollers, and leaf hoppers in rice fields [18]. Based on the characteristics of the biology, behaviour, and ecology of P. pseudoannulata, it has been concluded that this spider has a long growth and reproduction period, many egg laying periods, a large reproductive output, and high hatching and survival rates, and it is suitable for artificial breeding and has a high protection and utilisation value [3,10]. Food is essential to an animal’s behaviour and life history, including growth, reproduction, and survival [15,19]. The impact of spiders on natural and agro-ecosystems depends on the number of spiders as well as their habitat selection and foraging activities [20,21,22]. Therefore, it is crucial to understand the effects of factors such as prey nutrition on spider survival, foraging, growth, mating, and reproduction. It has been shown that the quality of prey affects the growth and reproduction of predatory arthropods, but little is known about the specific nutrients responsible for these effects [23,24]. Research on the nutritional ecology of spiders has mainly focused on how macronutrients such as protein and lipids affect the life history characteristics of spiders [9,25,26]. There are also some influences of trace elements. For instance, an important determinant of nutritional quality is the content of polyunsaturated fatty acids (PUFAs) [27]. The nutritional content of spiders themselves is directly related to the nutritional content of the food they prey upon [28,29]. Yeast contains a lot of nutrients, such as vitamins, proteins, fats, etc., which are needed for the survival of adult fruit flies and also promote the growth and development of their larvae.
The inclusion of yeast in the medium used for fruit-fly culture can promote the growth and reproduction of D. melanogaster [30,31]. The survival rate of D. melanogaster larvae is low when there is no yeast or low yeast intake. Increasing the ratio of protein to carbohydrate in the larval food can improve survival, shorten the larval development time, and increase adult quality [32,33,34,35]. Protein and carbohydrate are the two major macronutrients that exert profound influences over fitness in many organisms, including Drosophila melanogaster [36]. Studies have shown that altering the content of macronutrients in the medium in which D. melanogaster larvae develop results in changes in the macronutrient content in adult D. melanogaster, and these changes affect the survival, growth, and reproduction of spiders that feed on D. melanogaster [26,37,38,39,40,41,42]. In this paper, three treatments (no yeast, active yeast, and inactivated yeast) were applied to the conventional medium formulated for D. melanogaster. The effects of different yeast treatments on the pupation and emergence of D. melanogaster larvae, emergence rate, development time, adult body weight, and nutritional composition were analysed. The growth and development of P. pseudoannulata were also assessed after feeding on D. melanogaster grown on different media. We hypothesised that the presence of yeast in fruit-fly culture could improve the quality of the P. pseudoannulata. In order to rule out other influences, we only used D. melanogaster for feeding.

2. Materials and Methods

2.1. Collection and Rearing

We collected the Pardosa pseudoannulata in the rice field at Huazhong Agricultural University, Wuhan, Hubei Province, China, between September and December 2019 [18]. The collected P. pseudoannulata was reared at a temperature of 25 ± 1 °C, relative humidity of 60–70%, and light conditions of 14L:10D. A wet sponge was placed at the bottom of a glass finger tube for feeding, and the mouth of the tube was plugged with cotton. The spider was fed every 4 days with 10 fruit flies. The hatchlings underwent their first moult within the egg sacs. Only juveniles from the first clutch were assigned to the prey nutrient treatment groups (see below).

2.2. Generation of Fruit Flies with Different Nutrients

To generate fruit flies with different nutrients as prey for P. pseudoannulata juveniles, we prepared three types of culture medium. Group AY culture medium (activated yeast), the standard fruit-fly culture medium, comprised 240 mL H2O, 22 g corn powder, 16 g sucrose, 4 g yeast (Angel, Yichang, China), 1.6 g agar (Phygene, Fuzhou, China), 0.1 g benzoic acid (Sinopharm, Shanghai, China) (dissolved in 2 mL ethyl alcohol), and 1 mL propanoic acid (Sinopharm, Shanghai, China) [31]. Group IY culture medium contained 4 g of inactivated yeast. Group NY culture medium did not contain yeast. All other components of Groups IY and NY were identical to those of Group AY culture medium.

2.3. Juvenile Survival and Growth

Eight replicates per group were used for each of the three media. After the media were cooled and solidified, each medium was inoculated with five pairs of D. melanogaster within 8 h of fledging. D. melanogaster was transferred to a new glass tube 6 h after inoculation, and the larval duration, pupal duration, developmental duration, the number of pupae, and the number of flies emerged were observed and recorded each day. Forty newly emerged (unmated) D. melanogaster (female: male = 1:1) were randomly selected from three different media and frozen at −20 °C, and then each D. melanogaster was weighed using an analytical balance (Mettler Toledo, Greifensee, Switzerland). Since male and female D. melanogaster individuals are inherently different in size, we compared the effects of different treatments on female and male individuals separately.
The spiderlings (second instar) hatched from the egg sacs were divided into three groups, and each group consisted of 60 individuals. The juvenile spiders were housed individually in glass tubes (diameter × length: 20 × 60 mm) that were plugged with absorbent cotton. The spider was fed every 4 days with 10 fruit flies. The hatchlings underwent their first moult within the egg sacs. Only juveniles from the first clutch were assigned to the prey nutrient treatment groups (see below). The NY group was fed D. melanogaster that had been reared without yeast. The AY group was fed D. melanogaster that had been reared on medium containing activated yeast. The IY group was fed D. melanogaster that had been reared on medium containing high-temperature-inactivated yeast. In order to avoid the influence of other factors, only D. melanogaster was provided as food during the experiment. The spiderling was fed three times a week: five D. melanogaster each time. We replenished the water and cleaned any residue from the tube on each occasion. The moulting time and survival of P. pseudoannulata were recorded until they died. Twenty fifth-instar P. pseudoannulata in each of the three treatments were randomly selected. After being anaesthetised with CO2 gas, body length was measured under an optical microscope (SZX7), and they were weighed using a precision balance (BT1251).

2.4. Total Fat, Protein, and Glucose Were Measured

Five newly emerged D. melanogaster were randomly selected from the three different media and placed in a 1.5 mL centrifuge tube. We firstly added 50 μL 1 × PBS buffer into the test tube, and after the D. melanogaster was fully ground with three small steel balls, we used the buffer to rinse these steel balls into the test tube twice. Then, we shook the test tube violently with a turbine mixer for 1 min. Finally, we centrifuged the mixture in the test tube with a high-speed centrifuge at 4 °C, 10,000 r/min, for 10 min. A total of 200 μL of supernatant was taken for subsequent use, and each treatment was repeated six times. The content of protein was determined using a Protein Quantification Kit (Bradford Assay). The content of glucose was determined using the CheKineTM Glucose Assay Kit. The total fat content was determined by the acid hydrolysis method.
Ten spiderlings of the fifth instar were selected from the three treatment groups and stored in a −20 °C refrigerator, and the body substance content of each young spider was determined. The measurement method of nutrients in P. pseudoannulata was the same as for D. melanogaster.

2.5. Statistics

The difference in total fat, protein, and glucose content in D. melanogaster and P. pseudoannulata in different media was compared by one-way ANOVA. All values are reported as mean ± standard deviation (mean ± SD), unless otherwise stated. The developmental period, body length, and body weight of P. pseudoannulata were compared by one-way ANOVA. The difference in survival rate was measured by log-rank [43]. Statistical analysis of the data was performed using Excel and SPSS version 19 (IBM Corporation, Armonk, NY, USA), and GraphPad Prism 7 software was used for figure drawing.

3. Results

3.1. Growth and Development of Drosophila melanogaster

When comparing D. melanogaster fed with three different yeast treatments, significant differences were observed in the larval period (Table 1; one-way ANOVA: F2,33 = 14.933, p < 0.001), pupal period (F2,33 = 23.238, p < 0.001), total developmental time (F2,33 = 50.667, p < 0.001), the number of pupations (F2,33 = 235.823, p < 0.001), the number of eclosions (F2,33 = 158.205, p < 0.001), and the eclosion rate (F2,33 = 6.956, p = 0.01). Specifically, post hoc paired comparisons revealed that the pupal period (independent t-test: t = −3.024, df = 8, p = 0.016) and total developmental time of the AY group of D. melanogaster were significantly shorter than those of the IY group (t = −4.382, df = 8, p = 0.002). The larval period (p = 0.004), pupal period (p = 0.003), and total developmental time of the IY group of D. melanogaster were significantly shorter than those of the NY group (p < 0.001), while the larval period of the AY group did not differ significantly from that of the IY group (p = 0.215). The pupal period and total developmental time of D. melanogaster on activated yeast were significantly shorter than on inactive yeast. The number of pupations and eclosions of the AY group of D. melanogaster was significantly higher than that of the IY group (p < 0.001), while the eclosion rate did not differ significantly between these two groups (p = 0.616). The number of pupations (p < 0.001) and the number of eclosions (p < 0.001), as well as the eclosion rate of the IY group of D. melanogaster, were significantly higher than those of the NY group (p = 0.012).
Comparison of the weights of different sexes within the three groups of D. melanogaster showed significant differences (Figure 1; female: F2,166 = 31.463, p < 0.001; male: F2,150 = 28.556, p < 0.001). The weights of both male and female D. melanogaster in the AY group were significantly higher than those in the IY group (p < 0.001), while there was no significant difference in the weights of male D. melanogaster between the IY and NY groups (p = 0.231). However, there was a significant difference in the weights of female D. melanogaster (p = 0.01).

3.2. Survival and Growth of Spiderlings

The survival of juvenile P. pseudoannulata differed significantly according to the diet of the D. melanogaster on which they were reared (Figure 2; log-rank test: χ2= 18.23, df = 2, p < 0.001). The survival rate of juvenile spiders fed on the AY group of D. melanogaster was significantly lower than that of those fed on the IY group of D. melanogaster (χ2 = 4.481, p = 0.0343), and the survival rate of those fed on the IY group of D. melanogaster was significantly lower than that of those fed on the NY group of D. melanogaster (χ2 = 30.96, p < 0.001).
Overall, the prey type significantly affected the duration of the second instar of the spiderlings (Table 2; F2,36 = 5.330, p = 0.009) but had no significant impact on the developmental times of the third (F2,36 = 2.127, p = 0.134), fourth (F2,36 = 1.068, p = 0.354), and fifth instars (F2,36 = 2.000, p = 0.150). The developmental time of spiderlings feeding on AY fruit flies in the second instar was significantly lower than those feeding on IY fruit flies (p = 0.01), with no significant difference between the IY group and the NY group. Similarly, there were no significant differences in the body length (Figure 3a,b; F2,27 = 0.129, p = 0.879) or weight of the spiders between these two treatments (F2,27 = 0.030, p = 0.970).

3.3. The Content of Nutrients in D. melanogaster and P. pseudoannulata

There were significant differences in protein content (F2,32 = 20.076, p < 0.001), glucose content (F2,32 = 31.027, p < 0.001), and total fat content (F2,15 = 20.719, p < 0.001) (Figure 4) in D. melanogaster fed with the three different media. There were no significant differences in total fat (p = 0.196) or protein contents between the AY and IY groups (p = 0.086), but the IY group was significantly higher than the NY group (p < 0.001). The glucose content of D. melanogaster in the AY group was significantly higher than that in the IY group (p < 0.001), and that in the IY group was significantly higher than that in the NY group (p = 0.002).
The three different culture media-fed D. melanogaster had no significant impact on the glucose (F2,15 = 1.272, p = 0.309) or protein content in the P. pseudoannulata (F2,15 = 0.931, df = 2, p = 0.416) (Figure 5). However, there was a significant difference in total fat content (F2,15 = 81.521, p < 0.001). The total fat content in the P. pseudoannulata of the AY group was significantly higher than that of the IY group (p < 0.001), and the IY group was significantly higher than the NY group (p < 0.001).

4. Discussion

Research on the impact of food on the development of D. melanogaster usually focuses on the types and choices of food during the larval to adult stages or only during the adult stage, with little research involving the larval developmental period [41]. Studies have shown that the quantity and quality of yeast in larval food can affect the early development and adult life traits of D. melanogaster [44]. The body weight and length of D. melanogaster are important indicators of whether they are developing well [45]. In our experiment, we found that adding active yeast to the medium can shorten the larval period, pupal period, and total development time of D. melanogaster, as well as increase the number of pupations and eclosions and the eclosion rate of the flies, consistent with previously reported results [46,47]. The number of pupae in the group fed active yeast was significantly higher than in the other two groups, suggesting that D. melanogaster lay more eggs on medium with active yeast. Adding yeast to the medium can significantly increase the fat, protein, and glucose content of D. melanogaster. At the same time, it can increase the number of eggs laid by D. melanogaster. The number of days taken by eggs to develop into pupae is greatly reduced, indicating that yeast powder promotes the growth and development of D. melanogaster larvae [32]. Medium without added yeast gradually becomes dry over time, making it difficult for first-instar larvae to move and ultimately leading to developmental stagnation [48]. In the current study, the number of individuals in the F1 generation of D. melanogaster reared on the medium without added yeast was significantly lower than that of the other groups [49]. Related studies have shown that in the absence of yeast, larval survival rates are very low, but that reducing the yeast content in larval food can significantly prolong the lifespan of adults [50].
Food is crucial for the growth, reproduction, and survival of animals [51]. Although many studies have shown that the quality of prey can affect the growth and reproduction of predators [23], there has been little research on the effects of yeast on spiders via fruit-fly culture medium. The nutritional components of the prey can affect a wide range of life-history traits in spiders. In this study, the effects of nutrient content in fruit-fly medium on the growth and development of P. pseudoannulata were studied. Our study fed spiders with fruit flies reared on different yeast treatments, and the results showed that fruit flies fed with activated yeast shortened the developmental time of the second instar of P. pseudoannulata spiders, with no significant effects on other instars. P. pseudoannulata spiders that were fed fruit flies reared without yeast had the longest survival time. This result may be due to the smaller size and nutritional deficiencies of the flies in the no-yeast group. When the three treatment groups were fed the same number of flies, the spiders in the no-yeast group took in a relatively small amount of food. Numerous studies have shown that restricting food, in terms of both caloric and nutrient content, extends the life span of most animals to some extent, including yeast, nematodes, spiders, fruit flies, mice, rats, and rhesus monkeys [52,53,54]. It is possible to induce dietary restriction in captive female Frontinella pyramitela spiders by varying the number of Drosophila flies given to individual spiders (five, three, or one flies/week). DR increased mean longevity (respectively, 42, 63, and 81 days) [55].
Our experiments have also shown that food restriction can increase the lifespan of spiders. In this study, only fruit flies were fed to the spiders to exclude the influence of other factors. The survival and growth rates of spiders feeding exclusively on D. melanogaster significantly decrease [56,57]. A previous study of Hylyphantes graminicola revealed that a diet of high-lipid prey was associated with increased mortality during development [29]. Other studies on spiders have also shown that although a single food source initially results in high survival, growth, and developmental rates, spiders feeding exclusively on fruit flies cannot develop to reproductive maturity [58,59], suggesting that fruit flies may not be an adequate food source for mature spiders. For example, research has shown that when female Linyphiidae spiders are fed only a large number of fruit flies, their egg quality sharply decreases [9,26,60]. Mixed food sources may benefit predators, as they provide a wider range of nutrients [61,62]. Because the subjects of this study were fifth instar P. pseudoannulata spiders and their body size was small, the size difference among the three treatments was not significant. The growth rate and age length of second instar spiders respond more significantly to environmental changes (such as temperature and food) than those of spiderlings [63]. Other studies have reported significant differences in spider development with mixed food sources, while differences in those with single food sources were smaller [31].
Animals need to supplement various nutrient components to meet their nutritional needs, and their food-seeking behaviour is also influenced by the nutritional composition of the food. Knowledge of animal nutritional requirements can help in understanding their physiology, behaviour, ecology, and evolution [64,65]. Our experimental results showed that adding yeast to the culture medium could significantly increase the fat, protein, and glucose contents in D. melanogaster, while there was no difference in the protein and glucose contents in P. pseudoannulata as a predator, and the total fat content in D. melanogaster was only significantly increased when fed with yeast. Spiders can extract almost all lipids from their prey, but the protein absorption rate is low [66].
In summary, our study found that yeast promoted the growth and development of D. melanogaster larvae but shortened the developmental time of the second instar of P. pseudoannulata. The contents of glucose, protein, and total fat in D. melanogaster were significantly increased by adding yeast to the D. melanogaster culture medium, but only the contents of total fat in P. pseudoannulata. Our study illustrates the importance of yeast to the growth and development of fruit flies. Since a single food cannot provide sufficient nutrition for spiders, we will conduct mixed feeding until spiders mature in the follow-up experiment, and then study the effects of yeast on spiders through the food chain.

Author Contributions

Conceptualisation, Y.P. (Yaqi Peng) and R.L.; methodology, Y.P. (Yaqi Peng); software, Y.P. (Yaqi Peng); validation, R.L. and W.L.; formal analysis, W.L.; investigation, W.L.; resources, Y.P. (Yu Peng); data curation, Y.P. (Yaqi Peng); writing—original draft preparation, Y.P. (Yaqi Peng); writing—review and editing, Y.Z.; visualisation, Y.Z.; supervision, R.L. and Y.Z.; project administration, Y.P. (Yu Peng) and Y.Z.; funding acquisition, Y.P. (Yu Peng). All authors have read and agreed to the published version of the manuscript.

Funding

This work was funded by the National Natural Science Foundation of China (31672317), the Special Foundation for National Science and Technology Basic Research Programme of China (2018FY100400), and the Frontier Projects of the Applied Foundation of Wuhan Science and Technology Bureau (2019020701011464).

Data Availability Statement

Data will be made available on request.

Conflicts of Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

References

  1. Chatterjee, S.; Isaia, M.; Venturino, E. Spiders as biological controllers in the agroecosystem. J. Theor. Biol. 2009, 258, 352–362. [Google Scholar] [CrossRef]
  2. Samu, F.; Sunderland, K.D.; Szinetar, C. Scale-dependent dispersal and distribution patterns of spiders in agricultural systems: A review. J. Arachnol. 1999, 27, 325–332. [Google Scholar] [CrossRef]
  3. Yang, H.; Peng, Y.; Tian, J.; Wang, J.; Wei, B.; Xie, C.; Wang, Z. Rice field spiders in China: A review of the literature. J. Econ. Entomol. 2018, 111, 53–64. [Google Scholar] [CrossRef]
  4. Kaspari, M. The invisible hand of the periodic table: How micronutrients shape ecology. Annual Review of Ecology. Evol. Syst. 2021, 52, 199–219. [Google Scholar] [CrossRef]
  5. Sobczyk, Ł.; Filipiak, M.; Czarnoleski, M. Sexual dimorphism in the multielemental stoichiometric phenotypes and stoichiometric niches of spiders. Insects 2020, 11, 484. [Google Scholar] [CrossRef] [PubMed]
  6. Trubl, P.; Johnson, J.C. Ecological stoichiometry of the black widow spider and its prey from desert, urban and laboratory populations. J. Arid Environ. 2019, 163, 18–25. [Google Scholar] [CrossRef]
  7. Wilder, S.M.; Schneider, J.M. Micronutrient consumption by female Argiope bruennichi affects offspring survival. J. Insect Physiol. 2017, 100, 128–132. [Google Scholar] [CrossRef]
  8. Ludwig, L.; Barbour, M.A.; Guevara, J.; Avilés, L.; González, A.L. Caught in the web: Spider web architecture affects prey specialization and spider–prey stoichiometric relationships. Ecol. Evol. 2018, 8, 6449–6462. [Google Scholar] [CrossRef]
  9. Wilder, S.M. Spider Nutrition: An Integrative Perspective; Advances in Insect Physiology; Academic Press: Cambridge, MA, USA, 2011; Volume 40, pp. 87–136. [Google Scholar] [CrossRef]
  10. Michalko, R.; Pekár, S.; Entling, M.H. An updated perspective on spiders as generalist predators in biological control. Ecologia 2019, 189, 21–36. [Google Scholar] [CrossRef]
  11. Herzog, C.; Reeves, J.T.; Ipek, Y.; Jilling, A.; Hawlena, D.; Wilder, S.M. Multi-elemental consumer-driven nutrient cycling when predators feed on different prey. Oecologia 2023, 202, 1–14. [Google Scholar] [CrossRef]
  12. Jensen, K.; Mayntz, D.; Toft, S.; Raubenheimer, D.; Simpson, S.J. Prey nutrient composition has different effects on Pardosa wolf spiders with dissimilar life histories. Oecologia 2010, 165, 577–583. [Google Scholar] [CrossRef]
  13. Wilson, J.K.; Ruiz, L.; Davidowitz, G. Dietary protein and carbohydrates affect immune function and performance in a specialist herbivore insect (Manduca sexta). Physiol. Biochem. Zool. 2019, 92, 58–70. [Google Scholar] [CrossRef]
  14. Vargas, M.A.; Luo, N.; Yamaguchi, A.; Kapahi, P. A role for S6 kinase and serotonin in postmating dietary switch and balance of nutrients in D. melanogaster. Curr. Biol. 2010, 20, 1006–1011. [Google Scholar] [CrossRef]
  15. Raubenheimer, D.; Simpson, S.J.; Mayntz, D. Nutrition, ecology and nutritional ecology: Toward an integrated framework. Funct. Ecol. 2009, 23, 4–16. [Google Scholar] [CrossRef]
  16. Wasko, D.K.; Sasa, M. Food resources influence spatial ecology, habitat selection, and foraging behavior in an ambush-hunting snake (Viperidae: Bothrops asper): An experimental study. Zoology 2012, 115, 179–187. [Google Scholar] [CrossRef]
  17. Glaudas, X.; Alexander, G.J. Food supplementation affects the foraging ecology of a low-energy, ambush-foraging snake. Behav. Ecol. Sociobiol. 2017, 71, 5. [Google Scholar] [CrossRef]
  18. Zhao, J.Z. List of spiders in Chinese cotton fields (I). Acta Arachnol. Sin. 1992, 1, 23–30. [Google Scholar]
  19. Raubenheimer, D.; Simpson, S.J. Nutritional ecology and foraging theory. Curr. Opin. Insect Sci. 2018, 27, 38–45. [Google Scholar] [CrossRef]
  20. Settle, W.H.; Ariawan, H.; Astuti, E.T.; Cahyana, W.; Hakim, A.L.; Hindayana, D.; Lestari, A.S. Managing tropical rice pests through conservation of generalist natural enemies and alternative prey. Ecology 1996, 77, 1975–1988. [Google Scholar] [CrossRef]
  21. Barry, K.L.; Wilder, S.M. Macronutrient intake affects reproduction of a predatory insect. Oikos 2012, 122, 1058–1064. [Google Scholar] [CrossRef]
  22. Keswani, S.V. Spiders of agro-ecosystems: A review. Int. J. Multdiciplinary Res. 2023, 23, 1–18. [Google Scholar]
  23. Toft, S. Nutritional aspects of spider feeding. In Spider Ecophysiology; Springer: Berlin/Heidelberg, Germany, 2012; pp. 373–384. [Google Scholar] [CrossRef]
  24. Hou, F.; Ni, Z.H.; Zou, M.T.; Zhu, R.; Yi, T.C.; Guo, J.J.; Jin, D.C. The effects of alternative foods on life history and cannibalism of Amblyseius herbicolus (Acari: Phytoseiidae). Insects 2022, 13, 1036. [Google Scholar] [CrossRef]
  25. Hawley, J.; Simpson, S.J.; Wilder, S.M. Effects of prey macronutrient content on body composition and nutrient intake in a web-building spider. PLoS ONE 2014, 9, e99165. [Google Scholar] [CrossRef]
  26. Feng, Q.; Wen, L.; Ma, J.; Yu, L.; Li, C.; Jiao, X. The effects of prey lipid on female mating and reproduction of a wolf spider. Curr. Zool. 2022, 68, 726–733. [Google Scholar] [CrossRef]
  27. Kowarik, C.; Martin-Creuzburg, D.; Robinson, C.T. Cross-ecosystem linkages: Transfer of polyunsaturated fatty acids from streams to riparian spiders via emergent insects. Front. Ecol. Evol. 2021, 9, 707570. [Google Scholar] [CrossRef]
  28. Mayntz, D.; Toft, S. Nutrient composition of the prey’s diet affects growth and survivorship of a generalist predator. Oecologia 2001, 127, 207–213. [Google Scholar] [CrossRef]
  29. Wen, L.; Jiao, X.; Liu, F.; Zhang, S.; Li, D. High-lipid prey reduce juvenile survivorship and delay egg laying in a small linyphiid spider Hylyphantes graminicola. J. Exp. Biol. 2020, 223, jeb237255. [Google Scholar] [CrossRef]
  30. Günther, C.S.; Goddard, M.R. Do yeasts and Drosophila interact just by chance? Fungal Ecol. 2018, 38, 37–43. [Google Scholar] [CrossRef]
  31. Wen, L.; Zhang, Z.; Zhang, S.; Liu, F.; Jiao, X.; Li, D. Influence of maternal diet on offspring survivorship, growth, and reproduction in a sheetweb spider. Biol. Open 2020, 9, bio056846. [Google Scholar] [CrossRef]
  32. Xiu, B.; Wu, Q. Effects of yeast powder on fecundity and growth of Drosophila melanogaster. J. Tongji Univ. Med. Ed. 2002, 23, 204–206. [Google Scholar] [CrossRef]
  33. Wang, Z.L.; Wang, X.P.; Li, C.R.; Xia, Z.Z.; Li, S.X. Effect of dietary protein and carbohydrates on survival and growth in larvae of the Henosepilachna vigintioctopunctata (F.) Coleoptera: Coccinellidae. J. Insect Sci. 2018, 18, 3. [Google Scholar] [CrossRef]
  34. Cammack, J.A.; Tomberlin, J.K. The impact of diet protein and carbohydrate on select life-history traits of the black soldier fly Hermetia illucens (L.) (Diptera: Stratiomyidae). Insects 2017, 8, 56. [Google Scholar] [CrossRef]
  35. Miranda, C.D.; Cammack, J.A.; Tomberlin, J.K. Life-history traits of the black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), reared on three manure types. Animals 2019, 9, 281. [Google Scholar] [CrossRef]
  36. Jang, T.; Lee, K.P. Comparing the impacts of macronutrients on life-history traits in larval and adult Drosophila melanogaster: The use of nutritional geometry and chemically defined diets. J. Exp. Biol. 2018, 221, jeb181115. [Google Scholar] [CrossRef]
  37. Mayntz, D.; Toft, S.; Vollrath, F. Effects of prey quality and availability on the life history of a trap-building predator. Oikos 2003, 101, 631–638. [Google Scholar] [CrossRef]
  38. Toft, S.; Li, D.; Mayntz, D. A specialized araneophagic predator’s short-term nutrient utilization depends on the macronutrient content of prey rather than on prey taxonomic affiliation. Physiol. Entomol. 2010, 35, 317–327. [Google Scholar] [CrossRef]
  39. Jensen, K.; Mayntz, D.; Wang, T.; Simpson, S.J.; Overgaard, J. Metabolic consequences of feeding and fasting on nutritionally different diets in the wolf spider Pardosa prativaga. J. Insect Physiol. 2010, 56, 1095–1100. [Google Scholar] [CrossRef]
  40. Wiggins, W.D.; Wilder, S.M. Mismatch between dietary requirements for lipid by a predator and availability of lipid in prey. Oikos 2018, 127, 1024–1032. [Google Scholar] [CrossRef]
  41. Grangeteau, C.; Yahou, F.; Everaerts, C.; Dupont, S.; Farine, J.-P.; Beney, L.; Ferveur, J.-F. Yeast quality in juvenile diet affects Drosophila melanogaster adult life traits. Sci. Rep. 2018, 8, 1–9. [Google Scholar] [CrossRef]
  42. Kleinbaum, D.G.; Klein, M. Kaplan-Meier Survival curves and the Log-Rank Test. In Survival Analysis: A Self-Learning Text; Springer: New York, NY, USA, 2011; pp. 55–96. [Google Scholar] [CrossRef]
  43. Anderson, J.F. Responses to starvation in the spiders Lycosa lenta Hentz and Filistata hibernalis (Hentz). Ecology 1974, 55, 576–585. [Google Scholar] [CrossRef]
  44. Murgier, J.; Everaerts, C.; Farine, J.P.; Ferveur, J.F. Live yeast in juvenile diet induces species-specific effects on Drosophila adult behaviour and fitness. Sci. Rep. 2019, 9, 8873. [Google Scholar] [CrossRef]
  45. Hu, D.; Li, W.; Wang, J.; Peng, Y.Q.; Yun, Y.L.; Peng, Y. Interaction of high temperature stress and Wolbachia Infection on the Biological Characteristic of Drosophila melanogaster. Insects 2023, 14, 558. [Google Scholar] [CrossRef]
  46. Anagnostou, C.; Dorsch, M.; Rohlfs, M. Influence of dietary yeasts on Drosophila melanogaster life-history traits. Entomol. Exp. Appl. 2010, 136, 1–11. [Google Scholar] [CrossRef]
  47. Jiménez-Padilla, Y.; Adewusi, B.; Lachance, M.A.; Sinclair, B.J. Live yeasts accelerate Drosophila melanogaster larval development. J. Exp. Biol. 2024, 227, jeb247932. [Google Scholar] [CrossRef]
  48. Wang, N.; Xu, Y.Z.; Guo, X.Z.; Wang, B.; He, Q.Y. Effects of yeast culture addition on development and oviposition of Drosophila melanogaster. Anim. Husb. Feed Sci. 2018, 39, 16–18. [Google Scholar] [CrossRef]
  49. Mohapatra, A.K.; Pandey, P. Fecundity of inbred fruit fly Drosophila melanogaster on different solid culture media: An analysis. J. Appl. Nat. Sci. 2018, 10, 1109–1114. [Google Scholar] [CrossRef]
  50. Becher, P.G.; Flick, G.; Rozpędowska, E.; Schmidt, A.; Hagman, A.; Lebreton, S.; Bengtsson, M. Yeast, not fruit volatiles mediate Drosophila melanogaster attraction, oviposition and development. Funct. Ecol. 2012, 26, 822–828. [Google Scholar] [CrossRef]
  51. Pimentel, D.; Acquay, H.; Biltonen, M.; Rice, P.; Silva, M.; Nelson, J.; D’amore, M. Environmental and economic costs of pesticide use. BioScience 1992, 42, 750–760. [Google Scholar] [CrossRef]
  52. Mendelsohn, A.R.; Larrick, J.W. Dietary restriction: Critical co-factors to separate health span from life span benefits. Rejuvenation Res. 2012, 15, 523–529. [Google Scholar] [CrossRef]
  53. Rantisi, J.G. Transgenerational Inheritance of Starvation Effects on Lifespan and Reproduction in Caenorhabditis Elegans. Ph.D. Thesis, University of Oregon, Eugene, OR, USA, 2018. [Google Scholar]
  54. Joy, T.K.; Arik, A.J.; Corby-Harris, V.; Johnson, A.A.; Riehle, M.A. The impact of larval and adult dietary restriction on lifespan, reproduction and growth in the mosquito Aedes aegypti. Exp. Gerontol. 2010, 45, 685–690. [Google Scholar] [CrossRef]
  55. Le Bourg, É. Predicting whether dietary restriction would increase longevity in species not tested so far. Ageing Res. Rev. 2010, 9, 289–297. [Google Scholar] [CrossRef]
  56. Toft, S.; Wise, D.H. Growth, development, and survival of a generalist predator fed single-and mixed-species diets of different quality. Oecologia 1999, 119, 191–197. [Google Scholar] [CrossRef]
  57. Wen, L.; Wang, L.; Wang, Z.; Zhang, H.; Hu, L.; Peng, B.; Li, C. The Critical Role of Arachidonic Acid on Molting in Spiders. Curr. Zool. 2024, 71, 373–380. [Google Scholar] [CrossRef]
  58. Miyashita, K. Growth and development of Lycosa T-insignita Boes. et Str. (Araneae: Lycosidae) under different feeding conditions. Appl. Entomol. Zool. 1968, 3, 81–88. [Google Scholar] [CrossRef]
  59. Kleinteich, A.; Wilder, S.M.; Schneider, J.M. Contributions of juvenile and adult diet to the lifetime reproductive success and lifespan of a spider. Oikos 2015, 124, 130–138. [Google Scholar] [CrossRef]
  60. Toft, S. Value of the aphid Rhopalosiphum padi as food for cereal spiders. J. Appl. Ecol. 1995, 32, 552. [Google Scholar] [CrossRef]
  61. Lefcheck, J.S.; Whalen, M.A.; Davenport, T.M.; Stone, J.P.; Duffy, J.E. Physiological effects of diet mixing on consumer fitness: A meta-analysis. Ecology 2013, 94, 565–572. [Google Scholar] [CrossRef]
  62. Jensen, K.; Mayntz, D.; Toft, S.; Clissold, F.J.; Hunt, J.; Raubenheimer, D.; Simpson, S.J. Optimal foraging for specific nutrients in predatory beetles. Proc. R. Soc. B Biol. Sci. 2012, 279, 2212–2218. [Google Scholar] [CrossRef]
  63. Jakob, E.M.; Marshall, S.D.; Uetz, G.W. Estimating fitness: A comparison of body condition indices. Oikos 1996, 2, 61–67. [Google Scholar] [CrossRef]
  64. Matsuda, I.; Clauss, M.; Tuuga, A.; Sugau, J.; Hanya, G.; Yumoto, T.; Hummel, J. Factors affecting leaf selection by foregut-fermenting proboscis monkeys: New insight from in vitro digestibility and toughness of leaves. Sci. Rep. 2017, 7, 42774. [Google Scholar] [CrossRef]
  65. Li, Z.; Wei, Y.; Rogers, E. Food choice of white-headed langurs in Fusui, China. Int. J. Primatol. 2003, 24, 1189–1205. [Google Scholar] [CrossRef]
  66. Wilder, S.M.; Mayntz, D.; Toft, S.; Rypstra, A.L.; Pilati, A.; Vanni, M.J. Intraspecific variation in prey quality: A comparison of nutrient presence in prey and nutrient extraction by predators. Oikos 2010, 119, 350–358. [Google Scholar] [CrossRef]
Insects 16 00795 g001
Figure 1. Body weight of Drosophila melanogaster reared on different yeast treatments. Different letters indicate that there are significant differences between treatments (p < 0.05).
Figure 1. Body weight of Drosophila melanogaster reared on different yeast treatments. Different letters indicate that there are significant differences between treatments (p < 0.05).
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Insects 16 00795 g002
Figure 2. Survival rate and lifespan of Pardosa pseudoannulata fed Drosophila melanogaster reared on culture media with different yeast treatments (log-rank test, * p < 0.05).
Figure 2. Survival rate and lifespan of Pardosa pseudoannulata fed Drosophila melanogaster reared on culture media with different yeast treatments (log-rank test, * p < 0.05).
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Insects 16 00795 g003
Figure 3. The body weight and length of Pardosa pseudoannulata fed Drosophila melanogaster reared on different yeast treatments. (a) The body weights of different treatment groups of Pardosa pseudoannulata; (b) The body lengths of different treatment groups of Pardosa pseudoannulata.
Figure 3. The body weight and length of Pardosa pseudoannulata fed Drosophila melanogaster reared on different yeast treatments. (a) The body weights of different treatment groups of Pardosa pseudoannulata; (b) The body lengths of different treatment groups of Pardosa pseudoannulata.
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Figure 4. Total fat content (a), protein content (b), and glucose content (c) in Drosophila melanogaster treated with different yeasts. An asterisk indicates a highly significant difference between groups (LSD test, p < 0.05). The ns indicates no significant difference (LSD test, p > 0.05).
Figure 4. Total fat content (a), protein content (b), and glucose content (c) in Drosophila melanogaster treated with different yeasts. An asterisk indicates a highly significant difference between groups (LSD test, p < 0.05). The ns indicates no significant difference (LSD test, p > 0.05).
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Insects 16 00795 g005
Figure 5. Total fat content (a), protein content (b), and glucose content (c) of Pardosa pseudoannulata reared by Drosophila melanogaster from the culture media with different yeast treatments. An asterisk indicates a highly significant difference between groups (LSD test, p < 0.05). The ns indicates no significant difference (LSD test, p > 0.05).
Figure 5. Total fat content (a), protein content (b), and glucose content (c) of Pardosa pseudoannulata reared by Drosophila melanogaster from the culture media with different yeast treatments. An asterisk indicates a highly significant difference between groups (LSD test, p < 0.05). The ns indicates no significant difference (LSD test, p > 0.05).
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Table 1. Biological properties of D. melanogaster reared on different yeast treatments.
Table 1. Biological properties of D. melanogaster reared on different yeast treatments.
Treatment Group
Biological PropertiesNYAYIY
Larval duration (d)7.4 ± 0.55 a5.0 ± 0.71 b5.8 ± 0.84 b
Pupal duration (d)6.8 ± 0.84 a3.2 ± 0.84 c4.8 ± 0.84 b
Developmental duration (d)14.2 ± 1.10 a8.2 ± 0.84 c10.6 ± 0.89 b
The number of pupations62.8 ± 3.70 c118.4 ± 5.86 a99.4 ± 1.67 b
The number of eclosions49.6 ± 3.29 c100.4 ± 6.84 a83.40 ± 2.41 b
Eclosion rate (%)79.03 ± 0.04 b84.74 ± 0.02 a83.89 ± 0.01 a
The data are presented as mean ± SE. Different letters denote a significant difference (LSD test, p < 0.05).
Table 2. Developmental duration of Pardosa pseudoannulata fed Drosophila melanogaster reared on culture media with different yeast treatments (d).
Table 2. Developmental duration of Pardosa pseudoannulata fed Drosophila melanogaster reared on culture media with different yeast treatments (d).
Treatment Group
InstarNYAYIY
2nd instar14.31 ± 2.78 a11.15 ± 1.50 b14.08 ± 3.50 a
3rd instar7.69 ± 0.95 a8.61 ± 1.04 a8.31 ± 1.44 a
4th instar7.69 ± 0.75 a7.38 ± 0.51 a7.77 ± 0.83 a
5th instar11.08 ± 2.02 a10.62 ± 1.04 a9.92 ± 1.19 a
6th instar13.54 ± 3.23——
7th instar26.62 ± 3.66——
Different letters denote a significant difference ( p < 0.05).
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MDPI and ACS Style

Peng, Y.; Liu, R.; Li, W.; Zhao, Y.; Peng, Y. Effects of Yeast on the Growth and Development of Drosophila melanogaster and Pardosa pseudoannulata (Araneae: Lycsidae) Through the Food Chain. Insects 2025, 16, 795. https://doi.org/10.3390/insects16080795

AMA Style

Peng Y, Liu R, Li W, Zhao Y, Peng Y. Effects of Yeast on the Growth and Development of Drosophila melanogaster and Pardosa pseudoannulata (Araneae: Lycsidae) Through the Food Chain. Insects. 2025; 16(8):795. https://doi.org/10.3390/insects16080795

Chicago/Turabian Style

Peng, Yaqi, Rui Liu, Wei Li, Yao Zhao, and Yu Peng. 2025. "Effects of Yeast on the Growth and Development of Drosophila melanogaster and Pardosa pseudoannulata (Araneae: Lycsidae) Through the Food Chain" Insects 16, no. 8: 795. https://doi.org/10.3390/insects16080795

APA Style

Peng, Y., Liu, R., Li, W., Zhao, Y., & Peng, Y. (2025). Effects of Yeast on the Growth and Development of Drosophila melanogaster and Pardosa pseudoannulata (Araneae: Lycsidae) Through the Food Chain. Insects, 16(8), 795. https://doi.org/10.3390/insects16080795

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