Detection of La Crosse Virus In Situ and in Individual Progeny to Assess the Vertical Transmission Potential in Aedes albopictus and Aedes aegypti
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Mosquito Maintenance
2.2. Preparation of LACV-Containing Artificial Bloodmeals
2.3. Plaque Assays for the Detection of LACV
2.4. Immunofluorescence Confocal Microscopy (IFCM) to Detect LACV Antigen in Mosquito Tissues
2.5. Immunohistochemistry (IHC) Assay to Detect LACV Antigen In Situ
2.6. Oviposition of Individual LACV-Infected Females and Rearing of F1 Larvae for LACV Detection
2.7. RNA Extraction and cDNA Synthesis from Larvae
2.8. LACV TaqMan qRT-PCR
2.9. Statistical Analysis
3. Results
3.1. Detection of LACV in Midguts, Head Tissue, and Ovaries of Ae. aegypti and Ae. albopictus, Which Had Acquired a Single Virus-Containing Bloodmeal
3.2. LACV Vertical Transmission by Ae. albopictus and Ae. aegypti after One or Two Gonotrophic Cycles
3.3. Ovary Infection Patterns of LACV in HWE and LC Females following Acquisition of a Single Virus-Containing Bloodmeal (BM-1)
3.4. Observing Vertical LACV Transmission by Individual LC and HWE Females
3.5. Ovary Infection Patterns of LACV in Individual HWE and LC Females following Acquisition of BM-2 at 7- or 10-Days Post-BM-1
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mosquito | Number of Bloodmeals 1 | LACV-Infected Larva Pools (%) 2 |
---|---|---|
Ae. albopictus LC | BM-1 only | 0/15 (0) |
BM-2: 10-days post-BM-1 | 4/7 (57%) | |
Ae. aegypti HWE | BM-1 only | 0/27 (0) |
BM-2: 10-days post-BM-1 | 1/18 (6%) |
Mosquito | Days between BM-1 and BM-2 Ingestion 1 | Midgut Infection Rate (%) 2 | Ovary Infection Rate (%) 3 | Proportion of Females that Oviposited (%) | Average No. of Eggs Oviposited per Female 4 | Hatch Rate in % | VTR 5 |
---|---|---|---|---|---|---|---|
Aedes albopictus LC | 7 | 12/13 (92%) | 4/12 (33%) | 5/13 (38%) | 11 ± 2.2 | 32 ± 15.4 | 1/4 (25%) |
10 | 15/16 (94%) | 6/15 (40%) | 8/16 (50%) | 18 ± 8.9 | 48 ± 12.8 | 1/6 (17%) | |
Aedes aegypti HWE | 7 | 6/11 (55%) | 5/6 (83%) | 10/11 (91%) | 87 ± 9.9 | 64 ± 9.3 | 0/5 (0) |
10 | 3/12 (25%) | 2/3 (67%) | 8/12 (67%) | 95 ± 12.7 | 45 ± 11.4 | 0/2 (0) |
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Darby, C.S.; Featherston, K.M.; Lin, J.; Franz, A.W.E. Detection of La Crosse Virus In Situ and in Individual Progeny to Assess the Vertical Transmission Potential in Aedes albopictus and Aedes aegypti. Insects 2023, 14, 601. https://doi.org/10.3390/insects14070601
Darby CS, Featherston KM, Lin J, Franz AWE. Detection of La Crosse Virus In Situ and in Individual Progeny to Assess the Vertical Transmission Potential in Aedes albopictus and Aedes aegypti. Insects. 2023; 14(7):601. https://doi.org/10.3390/insects14070601
Chicago/Turabian StyleDarby, Christie S., Kyah M. Featherston, Jingyi Lin, and Alexander W. E. Franz. 2023. "Detection of La Crosse Virus In Situ and in Individual Progeny to Assess the Vertical Transmission Potential in Aedes albopictus and Aedes aegypti" Insects 14, no. 7: 601. https://doi.org/10.3390/insects14070601