Bacterial Symbionts in Ceratitis capitata
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ceratitis capitata Rearing
2.2. DNA Extraction
2.3. 16S rRNA Profiling
2.4. Molecular Detection of Asaia
2.5. Asaia Isolation from C. capitata Adults
2.6. Asaia sp. Transformation
2.7. Medfly Recolonization by Asaia Expressing Green Fluorescent Protein (Gfp)
2.8. Isolation of Bacteria from the ISPRA Population
2.9. Antagonistic Assay
3. Results
3.1. 16S rRNA Profiling
3.2. Detection, Isolation and Transformation of Asaia from C. capitata Populations
3.3. Medfly Recolonization Using Asaia Strain Expressing Green Fluorescent Protein (Gfp)
3.4. Bacteria Isolation from the ISPRA Population and Activity Test
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cappelli, A.; Petrelli, D.; Gasperi, G.; Serrao, A.G.M.; Ricci, I.; Damiani, C.; Favia, G. Bacterial Symbionts in Ceratitis capitata. Insects 2022, 13, 474. https://doi.org/10.3390/insects13050474
Cappelli A, Petrelli D, Gasperi G, Serrao AGM, Ricci I, Damiani C, Favia G. Bacterial Symbionts in Ceratitis capitata. Insects. 2022; 13(5):474. https://doi.org/10.3390/insects13050474
Chicago/Turabian StyleCappelli, Alessia, Dezemona Petrelli, Giuliano Gasperi, Aurelio Giuseppe Maria Serrao, Irene Ricci, Claudia Damiani, and Guido Favia. 2022. "Bacterial Symbionts in Ceratitis capitata" Insects 13, no. 5: 474. https://doi.org/10.3390/insects13050474
APA StyleCappelli, A., Petrelli, D., Gasperi, G., Serrao, A. G. M., Ricci, I., Damiani, C., & Favia, G. (2022). Bacterial Symbionts in Ceratitis capitata. Insects, 13(5), 474. https://doi.org/10.3390/insects13050474