Evidence of the Involvement of a Plus-C Odorant-Binding Protein HparOBP14 in Host Plant Selection and Oviposition of the Scarab Beetle Holotrichia parallela
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insect Rearing
2.2. Tissue-Specific RNA Extraction and cDNA Synthesis
2.3. Phylogenetic Analysis and Expression Levels of OBP Genes
2.4. Molecular Cloning of HparOBP14
2.5. Expression and Purification of Recombinant Protein
2.6. Fluorescence Displacement Binding Assay
2.7. RNAi-Mediated Gene Silencing
2.8. Electroantennogram (EAG) Recording
2.9. Y-Tube Olfactometer Behavioral Bioassays
2.10. Statistical Analysis
3. Results
3.1. Phylogenetic and Expression Level Analysis of 20 HparOBP Genes
3.2. Molecular Cloning HparOBP14
3.3. Tissue Expression Profile Analysis of HparOBP14
3.4. Purification of HparOBP14 Protein
3.5. Fluorescence Competitive Binding Assays
3.6. Transcription Level of HparOBP14 after dsRNA Injection
3.7. Electroantennogram Responses of Female H. parallela after dsRNA Injection
3.8. Effect of dsRNA Injection on the Behavior of H. parallela
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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HparOBP14 | |||
---|---|---|---|
Compound | IC50 (μmol/L) | Int (%) | Ki (μmol/L) |
6-methyl-5-hepten-2-one * | 32.04 | 61.66 | 28.21 |
octanal * | - | - | - |
dodecyl aldehyde * | - | 92.42 | - |
3-hexanol * | - | 84.27 | - |
cis-3-hexenyl formate * | - | 81.25 | - |
cis-3-hexenyl acetate * | - | 81.43 | - |
2-ethylhexanol * | - | 85.80 | - |
decanal & | - | 77.88 | - |
linalool & | - | 89.20 | - |
β-caryophyllen & | - | - | - |
(Z)-3-hexen-1-ol & | - | 96.53 | - |
1-nonanal & | - | 69.22 | - |
α-pinene & | 38.50 | 67.49 | 32.83 |
3-carene & | - | 68.52 | - |
camphene & | - | 83.88 | - |
α-phellandrene & | - | 83.00 | - |
1-hexanol § | - | 72.19 | - |
1-heptanol § | - | 83.41 | - |
eugenol § | - | 86.03 | - |
p-cresol § | - | 91.23 | - |
indole § | - | 82.23 | - |
3-methylindole § | 24.61 | 56.22 | 20.99 |
butyric acid § | - | 99.07 | - |
geraniol § | 39.98 | 72.07 | 34.10 |
p-cymene § | 29.90 | 62.70 | 25.50 |
formaldehyde § | 34.58 | 68.91 | 29.49 |
methanol § | 32.10 | 65.66 | 27.37 |
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Qu, Y.; Liu, X.; Zhao, X.; Qin, J.; Cao, Y.; Li, K.; Zhou, J.-J.; Wang, S.; Yin, J. Evidence of the Involvement of a Plus-C Odorant-Binding Protein HparOBP14 in Host Plant Selection and Oviposition of the Scarab Beetle Holotrichia parallela. Insects 2021, 12, 430. https://doi.org/10.3390/insects12050430
Qu Y, Liu X, Zhao X, Qin J, Cao Y, Li K, Zhou J-J, Wang S, Yin J. Evidence of the Involvement of a Plus-C Odorant-Binding Protein HparOBP14 in Host Plant Selection and Oviposition of the Scarab Beetle Holotrichia parallela. Insects. 2021; 12(5):430. https://doi.org/10.3390/insects12050430
Chicago/Turabian StyleQu, Yafei, Xiangyu Liu, Xu Zhao, Jianhui Qin, Yazhong Cao, Kebin Li, Jing-Jiang Zhou, Senshan Wang, and Jiao Yin. 2021. "Evidence of the Involvement of a Plus-C Odorant-Binding Protein HparOBP14 in Host Plant Selection and Oviposition of the Scarab Beetle Holotrichia parallela" Insects 12, no. 5: 430. https://doi.org/10.3390/insects12050430