Isolation and Characterization of Mosquito-Associated Spiroplasma cantharicola from Aedes japonicus Collected in Hokkaido, Japan
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Mosquito Sampling
2.2. Isolation of Filtrable Microorganisms from Field-Collected Mosquitoes
2.3. DNA Extraction
2.4. Polymerase Chain Reaction and Electrophoresis
2.5. Sequencing and Genetic Analysis
2.6. Culturing of S. cantharicola
2.7. Statistical Analyses
3. Results
3.1. Isolation and Identification of Filtrable Microorganism from a Field-Collected Mosquito
3.2. Prevalence of S. cantharicola in the Field-Collected Mosquitoes and Laboratory Colonies
3.3. Growth Kinetics of S. cantharicola
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Isolate in This Study (LC646116.1) | S. cantharicola Strain AR1357 (DQ861916.1) | Spiroplasma sp. Ar-1357 (AY189316.1) | S. cantharicola Strain CC-1 (NR_125516.1) | S. diminutum Strain CUAS-1 (NR_025702.1) | S. monobiae Strain MQ-1 (NR_104854.1) | S. floricola Strain 23-6 (NR_025703.1) | |
---|---|---|---|---|---|---|---|
S. cantharicola strain AR1357 (DQ861916.1) | 99.8% (1281/1283) | ||||||
Spiroplasma sp. Ar-1357 (AY189316.1) | 99.8% (1281/1283) | 99.8% (1502/1505) | |||||
S. cantharicola strain CC-1 (NR_125516.1) | 99.7% (1279/1283) | 99.9% (1503/1505) | 99.7% (1500/1504) | ||||
S. diminutum strain CUAS-1 (NR_025702.1) | 99.2% (1273/1283) | 98.9% (1351/1366) | 99.4% (1507/1516) | 99.3% (1493/1504) | |||
S. monobiae strain MQ-1 (NR_104854.1) | 99.1% (1272/1283) | 99.3% (1456/1467) | 99.3% (1457/1467) | 99.2% (1455/1467) | 98.9% (1451/1467) | ||
S. floricola strain 23-6 (NR_025703.1) | 98.8% (1268/1283) | 98.9% (1351/1366) | 99.0% (1364/1378) | 98.9% (1351/1366) | 99.0% (1364/1378) | 99.4% (1355/1363) | |
S. diabroticae strain DU-1 (NR_104751.1) | 98.8% (1267/1283) | 98.9% (1487/1504) | 99.0% (1493/1508) | 98.9% (1487/1504) | 99.2% (1496/1508) | 99.3% (1457/1467) | 99.7% (1366/1370) |
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Shimooka, M.; Sakurai, Y.; Muramatsu, Y.; Uchida, L. Isolation and Characterization of Mosquito-Associated Spiroplasma cantharicola from Aedes japonicus Collected in Hokkaido, Japan. Insects 2021, 12, 1056. https://doi.org/10.3390/insects12121056
Shimooka M, Sakurai Y, Muramatsu Y, Uchida L. Isolation and Characterization of Mosquito-Associated Spiroplasma cantharicola from Aedes japonicus Collected in Hokkaido, Japan. Insects. 2021; 12(12):1056. https://doi.org/10.3390/insects12121056
Chicago/Turabian StyleShimooka, Makoto, Yoshimi Sakurai, Yasukazu Muramatsu, and Leo Uchida. 2021. "Isolation and Characterization of Mosquito-Associated Spiroplasma cantharicola from Aedes japonicus Collected in Hokkaido, Japan" Insects 12, no. 12: 1056. https://doi.org/10.3390/insects12121056