Next Article in Journal
Bioactive Excreted/Secreted Products of Entomopathogenic Nematode Heterorhabditis bacteriophora Inhibit the Phenoloxidase Activity during the Infection
Next Article in Special Issue
A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in Lymantria Dispar L.
Previous Article in Journal
Limited Effect of Management on Apple Pollination: A Case Study from an Oceanic Island
Previous Article in Special Issue
Suppression of Transferrin Expression Enhances the Susceptibility of Plutella xylostella to Isaria cicadae
Open AccessArticle

Patterns of Occurrence and Activity of Entomopathogenic Fungi in the Algarve (Portugal) Using Different Isolation Methods

1
UDIT MED—Mediterranean Institute for Agriculture, Environment and Development, Pólo, Universidade do Algarve, Campus de Gambelas, Ed 8, 8005-139 Faro, Portugal
2
Departamento de Viticultura, Instituto de Ciencias de la Vid y del Vino (Gobierno de La Rioja, CSIC, Universidad de La Rioja), Finca La Grajera, Ctra. de Burgos Km. 6, 26007 Logroño, Spain
3
Departamento de Medioambiente, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA, Ctra. de la Coruña, km 7.5, 28040 Madrid, Spain
4
Departamento de Ciencias Biológicas e Biotecnologia, Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, Ed 8, 8005-139 Faro, Portugal
*
Author to whom correspondence should be addressed.
Insects 2020, 11(6), 352; https://doi.org/10.3390/insects11060352
Received: 29 April 2020 / Revised: 22 May 2020 / Accepted: 28 May 2020 / Published: 4 June 2020
(This article belongs to the Special Issue Biological Control and Insect Pathology)
Entomopathogenic fungi (EPF) are distributed in natural and agricultural soils worldwide. To investigate EPF occurrence in different botanical habitats and soil-ecoregions, we surveyed 50 georeferenced localities in the spring of 2016 across the Algarve region (South Portugal). Additionally, we compared three EPF isolation methods: insect baiting in untreated or pre-dried-soil and soil dilution plating on a selective medium. We hypothesized that forest habitats (oak and pine semi-natural areas) and the acidic soil ecoregion may favor EPF occurrence. Overall, EPF species were present in 68% of sites, widely distributed throughout the Algarve. The use of selective media resulted in higher recovery of EPF than did either soil-baiting method. Contrary to our hypothesis, neither vegetation type nor ecoregion appeared to influence EPF occurrence. Traditional and molecular methods confirmed the presence of five EPF species. Beauveria bassiana (34% of sites), was the most frequently detected EPF, using pre-dried soil baiting and soil dilution methods. However, baiting untreated soil recovered Fusarium solani more frequently (26% of sites), demonstrating the utility of using multiple isolation methods. We also found Fusarium oxysporum, Purpureocillium lilacinum and Metarhizium anisopliae in 14%, 8% and 2% of the sites, respectively. Three abiotic variables (pH, soil organic matter and Mg) explained 96% of the variability of the entomopathogen community (EPF and entomopathogenic nematodes) in a canonical correspondence analysis, confirming the congruence of the soil properties that drive the assemblage of both entomopathogens. This study expands the knowledge of EPF distribution in natural and cultivated Mediterranean habitats. View Full-Text
Keywords: biologic control; entomopathogenic organisms; habitat preference; Mediterranean agro-ecosystems; multivariate analysis biologic control; entomopathogenic organisms; habitat preference; Mediterranean agro-ecosystems; multivariate analysis
Show Figures

Graphical abstract

MDPI and ACS Style

Bueno-Pallero, F.Á.; Blanco-Pérez, R.; Vicente-Díez, I.; Rodríguez Martín, J.A.; Dionísio, L.; Campos-Herrera, R. Patterns of Occurrence and Activity of Entomopathogenic Fungi in the Algarve (Portugal) Using Different Isolation Methods. Insects 2020, 11, 352. https://doi.org/10.3390/insects11060352

AMA Style

Bueno-Pallero FÁ, Blanco-Pérez R, Vicente-Díez I, Rodríguez Martín JA, Dionísio L, Campos-Herrera R. Patterns of Occurrence and Activity of Entomopathogenic Fungi in the Algarve (Portugal) Using Different Isolation Methods. Insects. 2020; 11(6):352. https://doi.org/10.3390/insects11060352

Chicago/Turabian Style

Bueno-Pallero, Francisco Á.; Blanco-Pérez, Rubén; Vicente-Díez, Ignacio; Rodríguez Martín, José A.; Dionísio, Lídia; Campos-Herrera, Raquel. 2020. "Patterns of Occurrence and Activity of Entomopathogenic Fungi in the Algarve (Portugal) Using Different Isolation Methods" Insects 11, no. 6: 352. https://doi.org/10.3390/insects11060352

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop