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Open AccessArticle

Suppression of Transferrin Expression Enhances the Susceptibility of Plutella xylostella to Isaria cicadae

1
Key Laboratory of Biology and Green Control of Plant Diseases and Insect Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China
2
Crop Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
3
School of Horticulture, Anhui Agricultural University, Hefei 230036, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Insects 2020, 11(5), 281; https://doi.org/10.3390/insects11050281
Received: 3 March 2020 / Revised: 25 April 2020 / Accepted: 2 May 2020 / Published: 5 May 2020
(This article belongs to the Special Issue Biological Control and Insect Pathology)
Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a Trf (PxTrf) from Plutella xylostella was identified and characterized. The PxTrf consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric point of 7.18. Only a single iron domain was predicted in the N-lobe of PxTrf. Although PxTrf was expressed ubiquitously, the highest levels of expression were observed in the fourth instar larvae. PxTrf transcript level was highest in fat bodies among various tissues. The PxTrf transcript levels increased significantly after the stimulation of pathogens. A decrease in PxTrf expression via RNA interference enhanced the susceptibility of P. xylostella to the Isaria cicadae fungus and inhibited hemocyte nodulation in response to the fungal challenge. In addition, a considerable increase in the pupation rate was observed in larvae treated with double-stranded PxTrf (dsPxTrf). Overall, according to the results, PxTrf may participate in P. xylostella immunity against fungal infection and insect development. View Full-Text
Keywords: Plutella xylostella; Isaria cicadae; transferrin; expression patterns; immunity; development; RNAi Plutella xylostella; Isaria cicadae; transferrin; expression patterns; immunity; development; RNAi
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MDPI and ACS Style

Xu, H.; Hao, Z.; Wang, L.; Li, S.; Guo, Y.; Dang, X. Suppression of Transferrin Expression Enhances the Susceptibility of Plutella xylostella to Isaria cicadae. Insects 2020, 11, 281.

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