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Cloning, Expression Analysis, 20-Hydroxyecdysone Induction, and RNA Interference Study of Autophagy-Related Gene 8 from Heortia vitessoides Moore

College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
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Insects 2020, 11(4), 245; https://doi.org/10.3390/insects11040245
Received: 3 March 2020 / Revised: 1 April 2020 / Accepted: 8 April 2020 / Published: 15 April 2020
Autophagy is a highly conserved and regulated process in eukaryotic cells and remodels cytoplasm, recovers essential nutrients, and disposes of unwanted cytoplasmic components. Autophagy-related gene (ATG) 8, identified in Heortia vitessoides Moore, which is an oligophagous pest of Aquilaria sinensis (Lour.), was characterized (HvATG8). Multiple sequence alignment showed that HvATG8 possesses highly conserved domain structures. Stage- and tissue-specific expressions indicated that HvATG8 is highly expressed in prepupal, pupal, and adult stages and in the midgut of larvae and abdomen of adults. Lack of function of HvATG8 by RNA interference resulted in a significant decrease in survival rate and an increase in abnormal or nonviable phenotypes in H. vitessoides. Transition rate from larval to pupal stages was 33.0% and from pupal to adult stages was 15.0% after injection. Reduction of ATG8 expression reduced survival of H. vitessoides. Therefore, HvATG8 possibly plays a key role in normal growth stage of H. vitessoides. HvATG8 suppression downregulates HvATG3 expression, suggesting that the two genes are interconnected. Further, HvATG8 expression increased by 20-hydroxyecdysone treatment, starvation, and extreme temperature exposure. Starvation also altered expression of other ATGs in H. vitessoide. This study may be used to guide research on molecular mechanisms of autophagy in insects. View Full-Text
Keywords: autophagy; autophagy-related gene 8; RNA interference; 20-hydroxyecdysone; starvation; Heortia vitessoides Moore autophagy; autophagy-related gene 8; RNA interference; 20-hydroxyecdysone; starvation; Heortia vitessoides Moore
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Li, Z.; Lyu, Z.; Ye, Q.; Cheng, J.; Wang, C.; Lin, T. Cloning, Expression Analysis, 20-Hydroxyecdysone Induction, and RNA Interference Study of Autophagy-Related Gene 8 from Heortia vitessoides Moore. Insects 2020, 11, 245.

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