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Open AccessArticle

Transcriptome Analyses of Diaphorina citri Midgut Responses to Candidatus Liberibacter Asiaticus Infection

1
College of Life Sciences, Gannan Normal University, Ganzhou 341000, China
2
National Navel Orange Engineering Research Center, Gannan Normal University, Ganzhou 341000, China
3
Fruit Bureau of Ganzhou, Ganzhou 341000, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Insects 2020, 11(3), 171; https://doi.org/10.3390/insects11030171 (registering DOI)
Received: 4 February 2020 / Revised: 29 February 2020 / Accepted: 5 March 2020 / Published: 7 March 2020
The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), is an important transmission vector of the citrus greening disease Candidatus Liberibacter asiaticus (CLas). The D. citri midgut exhibits an important tissue barrier against CLas infection. However, the molecular mechanism of the midgut response to CLas infection has not been comprehensively elucidated. In this study, we identified 778 differentially expressed genes (DEGs) in the midgut upon CLas infection, by comparative transcriptome analyses, including 499 upregulated DEGs and 279 downregulated DEGs. Functional annotation analysis showed that these DEGs were associated with ubiquitination, the immune response, the ribosome, endocytosis, the cytoskeleton and insecticide resistance. KEGG enrichment analysis revealed that most of the DEGs were primarily involved in endocytosis and the ribosome. A total of fourteen DEG functions were further validated by reverse transcription quantitative PCR (RT-qPCR). This study will contribute to our understanding of the molecular interaction between CLas and D. citri. View Full-Text
Keywords: Diaphorina citri; transcriptome sequencing; CLas infection Diaphorina citri; transcriptome sequencing; CLas infection
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Yu, H.-Z.; Li, N.-Y.; Zeng, X.-D.; Song, J.-C.; Yu, X.-D.; Su, H.-N.; Chen, C.-X.; Yi, L.; Lu, Z.-J. Transcriptome Analyses of Diaphorina citri Midgut Responses to Candidatus Liberibacter Asiaticus Infection. Insects 2020, 11, 171.

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