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Open AccessArticle

Feeding Delivery of dsHvSnf7 Is a Promising Method for Management of the Pest Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae)

1
Key Laboratory of Bio-Pesticide Innovation and Application, South China Agricultural University, Guangdong Province, Guangzhou 510642, China
2
Engineering Research Center of Biocontrol, Ministry of Education and South China Agricultural University, Guangdong Province, Guangzhou 510642, China
3
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China
4
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
Authors to whom correspondence should be addressed.
Insects 2020, 11(1), 34; https://doi.org/10.3390/insects11010034
Received: 16 December 2019 / Revised: 25 December 2019 / Accepted: 30 December 2019 / Published: 31 December 2019
(This article belongs to the Special Issue RNAi in Insect Pest Control)
RNA interference (RNAi) techniques have emerged as powerful tools in the development of novel management strategies for the control of insect pests, such as Henosepilachna vigintioctopunctata, which is a major solanaceous pest in Asia. Our results showed that levels of HvSnf7 expression were greater in larval midguts than in other tissues. Silencing of HvSnf7 led to greater H. vigintioctopunctata mortality rates and appeared to be time- and partially dose-dependent. Bacterially expressed dsHvSnf7 that was applied to detached plant leaves caused 98, 88, and 60% mortality in 1st and 3rd instars, and adults after 10, 12, and 14 d, respectively; when applied to living plants, bacterially expressed dsHvSnf7 led to mortality in 1st and 3rd instars, with no effect on adults. Bacterially expressed dsHvSnf7 led to improved plant protection against H. vigintioctopunctata. Ultrastructural changes caused by HvSnf7-RNAi in larval midguts showed extensive loss of cellular contents that indicate loss of membrane integrity. This study indicate that HvSnf7 potentially can be used as RNAi target gene for controlling of H. vigintioctopunctata. View Full-Text
Keywords: Henosepilachna vigintioctopunctata; HvSnf7; feeding RNAi; mortality; ultrastructural change Henosepilachna vigintioctopunctata; HvSnf7; feeding RNAi; mortality; ultrastructural change
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Lü, J.; Liu, Z.; Guo, W.; Guo, M.; Chen, S.; Li, H.; Yang, C.; Zhang, Y.; Pan, H. Feeding Delivery of dsHvSnf7 Is a Promising Method for Management of the Pest Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae). Insects 2020, 11, 34.

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