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Open AccessArticle

Expression Dynamics of Core RNAi Machinery Genes in Pea Aphids Upon Exposure to Artificially Synthesized dsRNA and miRNAs

by Li Yang 1,2, Yuan Tian 1,2, Yuan-Yuan Peng 1,2, Jinzhi Niu 1,2 and Jin-Jun Wang 1,2,*
1
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China
2
State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
*
Author to whom correspondence should be addressed.
Insects 2020, 11(2), 70; https://doi.org/10.3390/insects11020070
Received: 2 December 2019 / Revised: 14 January 2020 / Accepted: 18 January 2020 / Published: 21 January 2020
(This article belongs to the Special Issue RNAi in Insect Pest Control)
The pea aphid is an important pest of vegetables and causes serious losses worldwide. RNA interference (RNAi) is an effective pest control tool, and three sub-pathways have been described: The miRNA pathway, siRNA pathway, and piRNA pathway. A large number of genes in miRNA pathway and piRNA pathway are found to be expanded. To study the roles of these genes, the expression of 25 core RNAi genes was screened in spatiotemporal samples, artificially synthesized dsRNA and miRNA treated samples. The 25 genes were all expressed during different development stages and in different tissues. In dsRNA-treated samples and miRNA-treated samples, the expressions of genes in these three pathways were induced, especially the expanded genes. This suggests a complex network of RNAi core genes in the three sub-pathways. Treatment of miRNA seems to induce gene expression in a dosage-dependent manner. These results increase our knowledge of the siRNA pathway and related factors from RNAi pathway in aphids and promote the use of RNAi for the control of aphid pests. View Full-Text
Keywords: pea aphids; miRNA pathway; siRNA pathway; piRNA pathway pea aphids; miRNA pathway; siRNA pathway; piRNA pathway
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Yang, L.; Tian, Y.; Peng, Y.-Y.; Niu, J.; Wang, J.-J. Expression Dynamics of Core RNAi Machinery Genes in Pea Aphids Upon Exposure to Artificially Synthesized dsRNA and miRNAs. Insects 2020, 11, 70.

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