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Open AccessArticle

Bioassays and Methodologies for Insecticide Tests with Larvae of Trogoderma granarium (Everts), the Khapra Beetle

1
USDA, ARS, Center for Grain and Animal Health Research, 1515 College Avenue, Manhattan, KS 66502, USA
2
Department of Entomology, Kansas State University, Manhattan, KS 66506, USA
3
Otis Laboratory USDA, APHIS, CPHST, 1398 West Truck Road, Buzzards Bay, MA 02542, USA
*
Author to whom correspondence should be addressed.
Insects 2019, 10(5), 145; https://doi.org/10.3390/insects10050145
Received: 18 April 2019 / Revised: 13 May 2019 / Accepted: 15 May 2019 / Published: 22 May 2019
(This article belongs to the Special Issue Improving Stored Product Insect Pest Management)
New insecticide treatment options would be beneficial for control programs for Trogoderma granarium Everts, the khapra beetle, in the United States. Two insecticides were evaluated, the Polyzone® formulation of deltamethrin and a formulation of the insect growth regulator methoprene combined with deltamethrin and the synergist piperonyl butoxide. In the test with Polyzone® deltamethrin, concrete arenas were treated with a low and high rate, and held outside, inside a shed, or inside a lab. Compared to storage in the lab, residue degradation increased slightly in the shed, and then further outside, as evidenced by greater larval survival and adult emergence. Across all environmental treatments, the high rate was more effective than the lower rate. For the combination methoprene product, the effect of food contact with treated surfaces was examined. When treating arenas with food and transferring the food to clean dishes, there was no immediate effect on larval survival, but there was a reduction in survival and emergence to the adult stage after one month. For both tests, larvae apparently often went into diapause after they were introduced onto the treatment arenas. Both treatments could be utilized in management programs if T. granarium infestations are detected. View Full-Text
Keywords: residual control; insecticides; testing procedures; stored products residual control; insecticides; testing procedures; stored products
MDPI and ACS Style

Arthur, F.H.; Domingue, M.J.; Scheff, D.S.; Myers, S.W. Bioassays and Methodologies for Insecticide Tests with Larvae of Trogoderma granarium (Everts), the Khapra Beetle. Insects 2019, 10, 145.

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