Knockdown of β-N-acetylglucosaminidase 2 Impairs Molting and Wing Development in Lasioderma serricorne (Fabricius)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Insect Culture
2.2. RNA Extraction and Cloning of LsNAG2 Gene
2.3. Sequence Analysis
2.4. Quantitative Real-Time PCR
2.5. RNA Interference
2.6. Statistical Analysis
3. Results
3.1. Identification and Characterization of LsNAG2
3.2. Spatiotemporal Expression Patterns of LsNAG2
3.3. Effects of LsNAG2 RNAi on the Expressions of Chitin Synthesis Genes
3.4. Effects of LsNAG2 RNAi on the Expressions of Wing Development-Related Genes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Yang, W.-J.; Xu, K.-K.; Yan, X.; Li, C. Knockdown of β-N-acetylglucosaminidase 2 Impairs Molting and Wing Development in Lasioderma serricorne (Fabricius). Insects 2019, 10, 396. https://doi.org/10.3390/insects10110396
Yang W-J, Xu K-K, Yan X, Li C. Knockdown of β-N-acetylglucosaminidase 2 Impairs Molting and Wing Development in Lasioderma serricorne (Fabricius). Insects. 2019; 10(11):396. https://doi.org/10.3390/insects10110396
Chicago/Turabian StyleYang, Wen-Jia, Kang-Kang Xu, Xin Yan, and Can Li. 2019. "Knockdown of β-N-acetylglucosaminidase 2 Impairs Molting and Wing Development in Lasioderma serricorne (Fabricius)" Insects 10, no. 11: 396. https://doi.org/10.3390/insects10110396