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Open AccessArticle

Bacteria-Mediated RNA Interference for Management of Plagiodera versicolora (Coleoptera: Chrysomelidae)

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, School of Life Sciences, Hubei University, Wuhan 430062, China
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Insects 2019, 10(12), 415; https://doi.org/10.3390/insects10120415
Received: 29 October 2019 / Revised: 13 November 2019 / Accepted: 15 November 2019 / Published: 21 November 2019
(This article belongs to the Special Issue RNAi in Insect Pest Control)
RNA interference (RNAi) has emerged as a novel and feasible strategy for pest management. Methods for cost-effective production and stable delivery of double-stranded RNA (dsRNA) to the target insects are crucial for the wide application of RNAi for pest control. In this study, we tested the expression of dsRNA in RNaseIII-deficient Escherichia coli HT115 which was then fed to Plagiodera versicolora larvae, an insect pest of Salicaceae plants worldwide. By targeting six potential genes, including actin (ACT), signal recognition particle protein 54k (SRP54), heat shock protein 70 (HSC70), shibire (SHI), cactus (CACT), and soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAP), we found that feeding bacteria-expressed dsRNA successfully triggered the silencing of the five target genes tested and the suppression of ACT and SRP54 genes caused significant mortality. Our results suggest that the oral delivery of bacteria-expressed dsRNA is a potential alternative for the control of P. versicolora, and that ACT and SRP54 genes are the potent targets. View Full-Text
Keywords: Plagiodera versicolora; RNA interference; double-stranded RNA; bacteria-expressed dsRNA; target genes Plagiodera versicolora; RNA interference; double-stranded RNA; bacteria-expressed dsRNA; target genes
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Zhang, Y.; Xu, L.; Li, S.; Zhang, J. Bacteria-Mediated RNA Interference for Management of Plagiodera versicolora (Coleoptera: Chrysomelidae). Insects 2019, 10, 415.

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