Metals and Trace Elements in Calcified Valves in Patients with Acquired Severe Aortic Valve Stenosis: Is There a Connection with the Degeneration Process?
Abstract
:1. Introduction
2. Material and Methods
3. Statistical Analysis
4. Results
5. Discussion
6. Conclusions
7. Limitation
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Characteristics, Comorbidity, Exposure Factor | Patients (n = 49) | p * | Controls (n = 34) | p * | p ** | ||
---|---|---|---|---|---|---|---|
Men (n = 25) | Women (n = 24) | 0.999 | Men (n = 20) | Women (n = 14) | 0.225 | 0.5101 | |
Age (years) | 70.8 ± 10.1 | 73.4 ± 5.9 | 0.01 | 50.25 ± 18.88 | 54.07 ± 15.45 | 0.5981 | 0.0001 |
BMI (kg/m2) | 30.0 ± 5.4 | 30.2 ± 5.4 | 0.999 | n/a | n/a | n/a | n/a |
Morphology of the aortic valve—number of cusps | |||||||
-Tricuspid | 6 (12.2%) | 7 (14.3%) | 0.753 | 20 (58.8%) | 14 (41.2%) | 0.999 | 0.0001 |
-Bicuspid | 4 (8.1%) | 4 (8.1%) | 0.999 | 0 (0%) | 0 (0%) | 0.999 | 0.0186 |
-Cannot be determined | 15 (30.6%) | 13 (26.5%) | 0.776 | 0 (0%) | 0 (0%) | 0.999 | 0.0001 |
Aortic valve area—AVA (cm2) | 0.8 ± 0.2 | 0.8 ± 0.2 | 0.999 | n/a | n/a | n/a | n/a |
Aortic valve area index—AVAi (cm2/m2) | 0.4 ± 0.1 | 0.4 ± 0.1 | 0.999 | n/a | n/a | n/a | n/a |
Aortic valve gradient maximal (mmHg) | 72.9 ± 16.5 | 73.4 ± 15.5 | 0.98 | n/a | n/a | n/a | n/a |
Aortic valve gradient mean (mmHg) | 44.2 ± 11.4 | 44.9 ± 10.6 | 0.98 | n/a | n/a | n/a | n/a |
Left ventricular ejection fraction—LVEF (%) | 55.6 ± 12.4 | 61.4 ± 7.6 | 0.07 | n/a | n/a | n/a | n/a |
Left ventricular ejection fraction—LVEF < 50% | 8 (16.3%) | 0 (0%) | 0.004 | n/a | n/a | n/a | n/a |
Comorbidity | |||||||
-Thoracic aorta dilatation ≥ 41 mm | 5 (10.2%) | 5 (10.2%) | 0.999 | n/a | n/a | n/a | n/a |
-Coronary artery disease (CAD) | 19 (38.7%) | 11 (22.4%) | 0.042 | 0 (0%) | 0 (0%) | 0.999 | 0.0001 |
-Atrial fibrillation (all forms) | 23 (46.9%) | 21 (42.8%) | 0.667 | n/a | n/a | n/a | n/a |
-Hypertension | 21 (42.8%) | 23 (46.9%) | 0.609 | n/a | n/a | n/a | n/a |
-Dyslipidemia | 17 (34.6%) | 16 (32.6%) | 0.999 | n/a | n/a | n/a | n/a |
-Diabetes mellitus | 12 (24.4%) | 9 (18.3%) | 0.567 | n/a | n/a | n/a | n/a |
-Malignancy | 2 (4%) | 3 (6.1%) | 0.67 | 0 (0%) | 0 (0%) | 0.999 | 0.0753 |
-Allergy | 6 (12.2%) | 8 (16.3%) | 0.47 | n/a | n/a | n/a | n/a |
Exposure factor | |||||||
-Amalgam filling | 21 (42.8%) | 22 (44.9%) | 0.667 | 17 (50%) | 12 (35.29%) | 0.999 | 0.7532 |
-False teeth (plastic with Ni-Co-Cr wire) | 13 (26.5%) | 14 (28.5%) | 0.776 | 7 (20.6%) | 5 (14.7%) | 0.999 | 0.1170 |
-Orthopedic implant | 4 (8.1%) | 5 (10.2%) | 0.725 | 1 (2.9%) | 1 (2.9%) | 0.999 | 0.1861 |
-Pacemaker, implantable cardioverter-defibrillator | 2 (4%) | 1 (2%) | 0.999 | 0 (0%) | 0 (0%) | 0.999 | 0.2656 |
-Coronary stent | 5 (10.2%) | 1 (2%) | 0.189 | 0 (0%) | 0 (0%) | 0.999 | 0.0767 |
-Occupational exposure 1 | 18 (36.7%) | 4 (8.1%) | 0.0001 | n/a | n/a | n/a | n/a |
-Smoking | 13 (26.5%) | 2 (4%) | 0.0015 | n/a | n/a | n/a | n/a |
Element | Unit | Patients (n = 49) | Controls (n = 34) | Median Ratio (P/C) | p | ||
---|---|---|---|---|---|---|---|
Median | Min.—Max. | Median | Min.—Max. | ||||
Al | µg/g | 0.4 | <0.4–2.3 | 0.6 | <0.4–20.0 | 0.7 | n/a |
Au | µg/g | 0.0010 | <0.005–0.0349 | <0.0005 | <0.0005–0.0552 | >2 | n/a |
Ba | µg/g | 4.57 | 2.08–11.6 | 0.112 | 0.03–1.53 | 40.8 | <0.0001 |
Ca | mg/g | 177 * | 78.9–243 * | 1.53 | 0.38–50.9 | 115.7 * | <0.0001 * |
Cd | µg/g | 0.0266 | 0.00355–0.115 | 0.1350 | 0.0191–0.583 | 0.2 | <0.0001 |
Co | µg/g | 0.0255 | 0.0063–0.112 | 0.0157 | 0.0044–0.149 | 1.6 | 0.0375 |
Cr | µg/g | 0.055 | 0.010–0.264 | 0.025 | 0.008–0.23 | 2.2 | 0.0014 |
Cu | µg/g | 0.79 | 0.40–2.42 | 2.09 | 0.55–4.44 | 0.4 | <0.0001 |
Hg | µg/g | <0.005 | <0.005–0.018 | <0.005 | <0.005–0.025 | n/a | n/a |
Mg | mg/g | 2.24 * | 1.01–3.03 * | 0.247 | 0.0914–0.994 | 9.1 * | <0.0001 * |
Mo | µg/g | 0.0086 | 0.0043–0.0254 | 0.0170 | 0.0065–0.0674 | 0.5 | <0.0001 |
Ni | µg/g | 0.012 | <0.009–0.110 | 0.011 | <0.009–0.11 | 1.1 | n/a |
P | mg/g | 86.9 * | 38.6–119 * | 2.37 | 1.36–29.4 | 36.7 * | <0.0001 * |
Pb | µg/g | 0.863 | 0.285–4.24 | 0.069 | 0.005–8.77 | 12.6 | <0.0001 |
S | mg/g | 3.30 | 2.23–5.29 | 3.89 | 1.15–5.27 | 0.8 | 0.0199 |
Se | µg/g | 0.345 | 0.195–0.829 | 0.296 | 0.118–0.436 | 1.2 | 0.0067 |
Sn | µg/g | 0.227 | 0.032–2.63 | 0.116 | 0.011–1.08 | 2.0 | 0.0008 |
Sr | µg/g | 59.5 | 29.7–125 | 0.969 | 0.207–27.90 | 61.4 | <0.0001 |
Ti | µg/g | <0.06 | <0.02–0.34 | <0.06 | <0.03–0.55 | n/a | n/a |
V | µg/g | 0.0010 | <0.0005–0.0032 | 0.0022 | <0.0005–0.0168 | 0.5 | n/a |
Zn | µg/g | 117 | 55–180 | 30.6 | 12.1–51.5 | 3.8 | <0.0001 |
Comorbidity, Characteristics (Patient Group) | Element | Median Concentration Ratio (>1) | p | Element | Median Concentration Ratio (<1) | p |
---|---|---|---|---|---|---|
Patients/Controls | Patients/Controls | |||||
Coronary artery disease (CAD) | Se | 0.3185/0.389 | 0.001 | |||
Dyslipidaemia | Zn | 125/103 | 0.0063 | |||
Chronic kidney disease (CKD) | Mo | 0.01035/0.008 | 0.0104 | |||
Pb | 1.16/0.7885 | 0.002 | ||||
Malignancy | Cr | 0.013/0.06 | 0.057 | |||
Smoking | Au | 0.0015/0.0009 | <0.0001 | Sn | 0.104/0.335 | 0.0002 |
Pb | 1.19/0.796 | <0.0001 | ||||
Gender—women | Cd | 0.0357/0.0233 | 0.0299 | Ca | 167.5/201 * | 0.0005 |
Co | 0.0318/0.019 | <0.0001 | P | 82.5/97.6 * | 0.0009 | |
Se | 0.385/0.293 | 0.0002 | Pb | 0.796/1.19 | 0.013 | |
Sn | 0.433/0.112 | 0.0062 | Zn | 106.5/131 | 0.013 | |
Gold crowns | Au | 0.0005/0.0014 | <0.0001 |
Patients | Controls | |||
---|---|---|---|---|
Element | Spearman r | p (Two-Tailed) | Spearman r | p (Two-Tailed) |
Ba | −0.087 | 0.548 | 0.571 | <0.001 |
Ca | 0.036 | 0.803 | 0.651 | <0.001 |
Cd | 0.031 | 0.830 | 0.321 | 0.064 |
Co | 0.130 | 0.372 | 0.439 | 0.009 |
Cr | −0.176 | 0.226 | 0.279 | 0.109 |
Cu | −0.166 | 0.253 | −0.230 | 0.189 |
Mg | 0.028 | 0.848 | 0.319 | 0.065 |
Mo | 0.186 | 0.199 | 0.0224 | 0.899 |
P | 0.008 | 0.954 | 0.462 | 0.005 |
Pb | 0.047 | 0.744 | 0.558 | 0.001 |
S | −0.062 | 0.670 | −0.241 | 0.169 |
Se | −0.047 | 0.743 | −0.179 | 0.311 |
Sn | −0.055 | 0.704 | 0.0401 | 0.821 |
Sr | 0.114 | 0.431 | 0.771 | <0.001 |
Zn | 0.117 | 0.420 | 0.098 | 0.580 |
Element | Unit | Men (n = 25) | Women (n = 24) | Median Ratio (M/W) | p | ||
---|---|---|---|---|---|---|---|
Median | Min.—Max. | Median | Min.—Max. | ||||
Al | µg/g | 0.6 | <0.4–1.4 | 0.7 | <0.4–2.3 | 0.9 | n/a |
Au | µg/g | 0.0014 | <0.005–0.0315 | 0.00145 | <0.005–0.0349 | 1.0 | n/a |
Ba | µg/g | 4.29 | 2.56–9.45 | 4.645 | 2.08–11.6 | 0.9 | 0.7851 |
Ca | mg/g | 189.0 * | 78.9–243 * | 169.0 * | 99.8–211 * | 1.1 * | 0.0046 * |
Cd | µg/g | 0.0208 | 0.00355–0.079 | 0.03475 | 0.016–0.115 | 0.6 | 0.0103 |
Co | µg/g | 0.0168 | 0.0063–0.0701 | 0.03155 | 0.0103–0.112 | 0.5 | <0.0001 |
Cr | µg/g | 0.05 | 0.010–0.264 | 0.058 | 0.011–0.201 | 0.9 | 0.059 |
Cu | µg/g | 0.75 | 0.40–2.42 | 0.85 | 0.58–1.42 | 0.9 | 0.0512 |
Hg | µg/g | 0.006 | <0.005–0.018 | 0.001 | <0.005–0.002 | n/a | n/a |
Mg | mg/g | 2.39 * | 1.01–3.03 * | 2.195 * | 1.14–2.96 * | 1.1 * | 0.0242 * |
Mo | µg/g | 0.0087 | 0.0043–0.0254 | 0.0082 | 0.0055–0.0132 | 1.1 | 0.5223 |
Ni | µg/g | 0.02 | <0.009–0.03 | 0.018 | 0.01–0.11 | 1.1 | n/a |
P | mg/g | 91.9 * | 38.6–119 * | 83.45 * | 49.1–106.0 * | 1.1 * | 0.0059 * |
Pb | µg/g | 1.09 | 0.5–4.24 | 0.7885 | 0.285–3.8 | 1.4 | 0.0327 |
S | mg/g | 3.13 | 2.23–4.97 | 3.49 | 2.8–5.29 | 0.9 | 0.0016 |
Se | µg/g | 0.317 | 0.195–0.829 | 0.3850 | 0.3–0.482 | 0.8 | 0.0033 |
Sn | µg/g | 0.184 | 0.032–0.977 | 0.383 | 0.06–2.63 | 0.5 | 0.0157 |
Sr | µg/g | 58.3 | 29.7–125 | 60.55 | 36.2–118 | 1.0 | 0.9487 |
Ti | µg/g | 0.07 | 0.03–0.27 | 0.07 | 0.02–0.34 | 1.0 | 0.3843 |
V | µg/g | 0.0014 | <0.0005–0.0029 | 0.0012 | <0.0005–0.0032 | 1.2 | n/a |
Zn | µg/g | 125.0 | 55–180 | 107.5 | 73.0–173 | 1.2 | 0.0191 |
Element | Unit | Men (n = 20) | Women (n = 14) | Median Ratio (M/W) | p | ||
---|---|---|---|---|---|---|---|
Median | Min.—Max. | Median | Min.—Max. | ||||
Al | µg/g | 0.75 | 0.3–20 | 0.95 | 0.2–2.2 | 0.8 | 0.3401 |
Au | µg/g | <0.005 | <0.005–0.055 | <0.005 | <0.005 | n/a | n/a |
Ba | µg/g | 0.1325 | 0.032–1.53 | 0.0905 | 0.03–0.34 | 1.5 | 0.0618 |
Ca | mg/g | 1.95 | 0.44–50.9 | 1.525 | 0.38–4.74 | 1.3 | 0.4311 |
Cd | µg/g | 0.132 | 0.0191–0.55 | 0.1955 | 0.0496–0.583 | 0.7 | 0.1101 |
Co | µg/g | 0.01425 | 0.0044–0.149 | 0.02025 | 0.0086–0.0431 | 0.7 | 0.4109 |
Cr | µg/g | 0.023 | 0.009–0.23 | 0.032 | 0.008–0.066 | 0.7 | 0.528 |
Cu | µg/g | 1.99 | 1.22–3.03 | 2.15 | 0.549–4.44 | 0.9 | 0.528 |
Hg | µg/g | 0.0065 | <0.005–0.016 | 0.011 | <0.005–0.025 | n/a | n/a |
Mg | mg/g | 0.255 | 0.149–0.994 | 0.239 | 0.0914–0.379 | 1.1 | 0.3274 |
Mo | µg/g | 0.0178 | 0.0097–0.0374 | 0.01635 | 0.0065–0.0674 | 1.1 | 0.6346 |
Ni | µg/g | 0.012 | <0.009–0.11 | 0.022 | <0.009–0.057 | n/a | n/a |
P | mg/g | 2.385 | 1.36–29.4 | 2.255 | 1.57–3.93 | 1.1 | 0.421 |
Pb | µg/g | 0.1085 | 0.012–2.57 | 0.054 | 0.005–8.77 | 2.0 | 0.3632 |
S | mg/g | 3.82 | 2.47–5.27 | 3.93 | 1.15–4.93 | 1.0 | 0.8427 |
Se | µg/g | 0.2885 | 0.187–0.436 | 0.3255 | 0.118–0.429 | 0.9 | 0.6472 |
Sn | µg/g | 0.1105 | 0.011–0.558 | 0.131 | 0.022–1.08 | 0.8 | 0.9656 |
Sr | µg/g | 0.974 | 0.392–27.9 | 0.939 | 0.207–2.66 | 1.0 | 0.3275 |
Ti | µg/g | 0.08 | 0.04–0.55 | 0.06 | 0.03–0.16 | 1.3 | 0.99 |
V | µg/g | 0.0022 | 0.001–0.0168 | 0.0029 | <0.0005–0.0155 | n/a | n/a |
Zn | µg/g | 31.15 | 17.8–51.5 | 30.15 | 12.1–43.1 | 1.0 | 0.6103 |
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Tomášek, A.; Maňoušek, J.; Kuta, J.; Hlásenský, J.; Křen, L.; Šindler, M.; Zelený, M.; Kala, P.; Němec, P. Metals and Trace Elements in Calcified Valves in Patients with Acquired Severe Aortic Valve Stenosis: Is There a Connection with the Degeneration Process? J. Pers. Med. 2023, 13, 320. https://doi.org/10.3390/jpm13020320
Tomášek A, Maňoušek J, Kuta J, Hlásenský J, Křen L, Šindler M, Zelený M, Kala P, Němec P. Metals and Trace Elements in Calcified Valves in Patients with Acquired Severe Aortic Valve Stenosis: Is There a Connection with the Degeneration Process? Journal of Personalized Medicine. 2023; 13(2):320. https://doi.org/10.3390/jpm13020320
Chicago/Turabian StyleTomášek, Aleš, Jan Maňoušek, Jan Kuta, Jiří Hlásenský, Leoš Křen, Martin Šindler, Michal Zelený, Petr Kala, and Petr Němec. 2023. "Metals and Trace Elements in Calcified Valves in Patients with Acquired Severe Aortic Valve Stenosis: Is There a Connection with the Degeneration Process?" Journal of Personalized Medicine 13, no. 2: 320. https://doi.org/10.3390/jpm13020320