Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis

Annesi, Ferdinanda; Hermoso-Durán, Sonia; Rizzuti, Bruno; Bruno, Rosalinda; Pirritano, Domenico; Petrone, Alfredo; Del Giudice, Francesco; Ojeda, Jorge; Vega, Sonia; Sanchez-Gracia, Oscar; Velazquez-Campoy, Adrian; Abian, Olga; Guzzi, Rita

doi:10.3390/jpm11040295

Open AccessArticle

Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis

by

Ferdinanda Annesi

^1,†,

Sonia Hermoso-Durán

^2,3,†

,

Bruno Rizzuti

^1,2

,

Rosalinda Bruno

⁴,

Domenico Pirritano

⁵,

Alfredo Petrone

⁵,

Francesco Del Giudice

⁵,

Jorge Ojeda

⁶,

Sonia Vega

²,

Oscar Sanchez-Gracia

⁷,

Adrian Velazquez-Campoy

^2,3,8,9,10

,

Olga Abian

^2,3,8,9,11,*

and

Rita Guzzi

^1,12,*

¹

CNR-NANOTEC, Licryl-UOS Cosenza and CEMIF.Cal, Department of Physics, University of Calabria, 87036 Rende, Italy

²

Institute of Biocomputation and Physics of Complex Systems (BIFI), Joint Units IQFR-CSIC-BIFI, and GBsC-CSIC-BIFI, Universidad de Zaragoza, 50018 Zaragoza, Spain

³

Instituto de Investigación Sanitaria Aragón (IIS Aragón), 50009 Zaragoza, Spain

⁴

Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Italy

⁵

Neurological and Stroke Unit, Multiple Sclerosis Clinic, Annunziata Hospital, 87100 Cosenza, Italy

⁶

Department of Statistical Methods, Universidad de Zaragoza, 50009 Zaragoza, Spain

⁷

SOTER BioAnalytics, Enrique Val, 50011 Zaragoza, Spain

⁸

Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, 50009 Zaragoza, Spain

⁹

Centro de Investigación Biomédica en Red en el Área Temática de Enfermedades Hepáticas y Digestivas (CIBERehd), 28029 Madrid, Spain

¹⁰

Fundación ARAID, Gobierno de Aragón, 50009 Zaragoza, Spain

¹¹

Instituto Aragonés de Ciencias de la Salud (IACS), 50009 Zaragoza, Spain

¹²

Department of Physics, Molecular Biophysics Laboratory, University of Calabria, 87036 Rende, Italy

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^*

Authors to whom correspondence should be addressed.

^†

Co-first authors of the manuscript.

J. Pers. Med. 2021, 11(4), 295; https://doi.org/10.3390/jpm11040295

Submission received: 25 January 2021 / Revised: 31 March 2021 / Accepted: 9 April 2021 / Published: 13 April 2021

(This article belongs to the Special Issue Personalized Medicine for Multiple Sclerosis)

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Abstract

Background: Multiple sclerosis (MS) is frequently characterized by a variety of clinical signs, often exhibiting little specificity. The diagnosis requires a combination of medical observations and instrumental tests, and any support for its objective assessment is helpful. Objective: Herein, we describe the application of thermal liquid biopsy (TLB) of blood plasma samples, a methodology for predicting the occurrence of MS with a noninvasive, quick blood test. Methods: TLB allows one to define an index (TLB score), which provides information about overall real-time alterations in plasma proteome that may be indicative of MS. Results: This pilot study, based on 85 subjects (45 MS patients and 40 controls), showed good performance indexes (sensitivity and specificity both around 70%). The diagnostic methods better discriminate between early stage and low-burden MS patients, and it is not influenced by gender, age, or assumption of therapeutic drugs. TLB is more accurate for patients having low disability level (≤ 3.0, measured by the expanded disability status scale, EDSS) and a relapsing–remitting diagnosis. Conclusion: Our results suggest that TLB can be applied to MS, especially in an initial phase of the disease when diagnosis is difficult and yet more important (in such cases, accuracy of prediction is close to 80%), as well as in personalized patient periodic monitoring. The next step will be determining its utility in differentiating between MS and other disorders, in particular in inflammatory diseases.

Keywords: multiple sclerosis; thermal liquid biopsy; plasma proteome profile; differential scanning calorimetry; multivariate analysis

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MDPI and ACS Style

Annesi, F.; Hermoso-Durán, S.; Rizzuti, B.; Bruno, R.; Pirritano, D.; Petrone, A.; Del Giudice, F.; Ojeda, J.; Vega, S.; Sanchez-Gracia, O.; et al. Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis. J. Pers. Med. 2021, 11, 295. https://doi.org/10.3390/jpm11040295

AMA Style

Annesi F, Hermoso-Durán S, Rizzuti B, Bruno R, Pirritano D, Petrone A, Del Giudice F, Ojeda J, Vega S, Sanchez-Gracia O, et al. Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis. Journal of Personalized Medicine. 2021; 11(4):295. https://doi.org/10.3390/jpm11040295

Chicago/Turabian Style

Annesi, Ferdinanda, Sonia Hermoso-Durán, Bruno Rizzuti, Rosalinda Bruno, Domenico Pirritano, Alfredo Petrone, Francesco Del Giudice, Jorge Ojeda, Sonia Vega, Oscar Sanchez-Gracia, and et al. 2021. "Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis" Journal of Personalized Medicine 11, no. 4: 295. https://doi.org/10.3390/jpm11040295

APA Style

Annesi, F., Hermoso-Durán, S., Rizzuti, B., Bruno, R., Pirritano, D., Petrone, A., Del Giudice, F., Ojeda, J., Vega, S., Sanchez-Gracia, O., Velazquez-Campoy, A., Abian, O., & Guzzi, R. (2021). Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis. Journal of Personalized Medicine, 11(4), 295. https://doi.org/10.3390/jpm11040295

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Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis

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