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Article

Increased Expression of VANGL1 is Predictive of Lymph Node Metastasis in Colorectal Cancer: Results from a 20-Gene Expression Signature

by
Noshad Peyravian
1,
Stefania Nobili
2,
Zahra Pezeshkian
1,
Meysam Olfatifar
1,
Afshin Moradi
3,
Kaveh Baghaei
1,
Fakhrosadat Anaraki
4,
Kimia Nazari
1,
Hamid Asadzadeh Aghdaei
1,
Mohammad Reza Zali
5,
Enrico Mini
6,* and
Ehsan Nazemalhosseini Mojarad
5,*
1
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, P.O. Box 19875-17411, Tehran, Iran
2
Department of Neurosciences, Imaging and Clinical Sciences, “G. D’Annunzio” University of Chieti-Pescara, 66100 Chieti, Italy
3
Department of Pathology, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4
Colorectal Division of Department of Surgery, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Yaman Street, Chamran Expressway, P.O. Box 19857-17411, Tehran, Iran
6
Department of Health Sciences, University of Florence, Viale Pieraccini, 6 – 50139 Firenze, Italy
*
Authors to whom correspondence should be addressed.
J. Pers. Med. 2021, 11(2), 126; https://doi.org/10.3390/jpm11020126
Submission received: 26 December 2020 / Revised: 30 January 2021 / Accepted: 7 February 2021 / Published: 14 February 2021
(This article belongs to the Special Issue Gastrointestinal Cancers and Personalized Medicine)
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Abstract

This study aimed at building a prognostic signature based on a candidate gene panel whose expression may be associated with lymph node metastasis (LNM), thus potentially able to predict colorectal cancer (CRC) progression and patient survival. The mRNA expression levels of 20 candidate genes were evaluated by RT-qPCR in cancer and normal mucosa formalin-fixed paraffin-embedded (FFPE) tissues of CRC patients. Receiver operating characteristic curves were used to evaluate the prognosis performance of our model by calculating the area under the curve (AUC) values corresponding to stage and metastasis. A total of 100 FFPE primary tumor tissues from stage I–IV CRC patients were collected and analyzed. Among the 20 candidate genes we studied, only the expression levels of VANGL1 significantly varied between patients with and without LNMs (p = 0.02). Additionally, the AUC value of the 20-gene panel was found to have the highest predictive performance (i.e., AUC = 79.84%) for LNMs compared with that of two subpanels including 5 and 10 genes. According to our results, VANGL1 gene expression levels are able to estimate LNMs in different stages of CRC. After a proper validation in a wider case series, the evaluation of VANGL1 gene expression and that of the 20-gene panel signature could help in the future in the prediction of CRC progression.
Keywords: colorectal cancer; gene signature; mRNA expression; VANGL1; FFPE colorectal cancer; gene signature; mRNA expression; VANGL1; FFPE
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MDPI and ACS Style

Peyravian, N.; Nobili, S.; Pezeshkian, Z.; Olfatifar, M.; Moradi, A.; Baghaei, K.; Anaraki, F.; Nazari, K.; Asadzadeh Aghdaei, H.; Zali, M.R.; et al. Increased Expression of VANGL1 is Predictive of Lymph Node Metastasis in Colorectal Cancer: Results from a 20-Gene Expression Signature. J. Pers. Med. 2021, 11, 126. https://doi.org/10.3390/jpm11020126

AMA Style

Peyravian N, Nobili S, Pezeshkian Z, Olfatifar M, Moradi A, Baghaei K, Anaraki F, Nazari K, Asadzadeh Aghdaei H, Zali MR, et al. Increased Expression of VANGL1 is Predictive of Lymph Node Metastasis in Colorectal Cancer: Results from a 20-Gene Expression Signature. Journal of Personalized Medicine. 2021; 11(2):126. https://doi.org/10.3390/jpm11020126

Chicago/Turabian Style

Peyravian, Noshad, Stefania Nobili, Zahra Pezeshkian, Meysam Olfatifar, Afshin Moradi, Kaveh Baghaei, Fakhrosadat Anaraki, Kimia Nazari, Hamid Asadzadeh Aghdaei, Mohammad Reza Zali, and et al. 2021. "Increased Expression of VANGL1 is Predictive of Lymph Node Metastasis in Colorectal Cancer: Results from a 20-Gene Expression Signature" Journal of Personalized Medicine 11, no. 2: 126. https://doi.org/10.3390/jpm11020126

APA Style

Peyravian, N., Nobili, S., Pezeshkian, Z., Olfatifar, M., Moradi, A., Baghaei, K., Anaraki, F., Nazari, K., Asadzadeh Aghdaei, H., Zali, M. R., Mini, E., & Nazemalhosseini Mojarad, E. (2021). Increased Expression of VANGL1 is Predictive of Lymph Node Metastasis in Colorectal Cancer: Results from a 20-Gene Expression Signature. Journal of Personalized Medicine, 11(2), 126. https://doi.org/10.3390/jpm11020126

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