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Open AccessArticle

Visual Detection of Dengue-1 RNA Using Gold Nanoparticle-Based Lateral Flow Biosensor

1
Nano-Biosensors Lab, Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA
2
Department of Chemistry, University of San Carlos, Talamban, Cebu 6000, Philippines
3
Department of Chemistry, Silliman University, Dumaguete 6200, Philippines
*
Author to whom correspondence should be addressed.
Diagnostics 2019, 9(3), 74; https://doi.org/10.3390/diagnostics9030074
Received: 6 June 2019 / Revised: 2 July 2019 / Accepted: 10 July 2019 / Published: 11 July 2019
(This article belongs to the Special Issue Biosensors-Based Diagnostics)
Dengue is a rapidly spreading mosquito-borne viral disease. Early diagnosis is important for clinical screening, medical management, and disease surveillance. The objective of this study was to develop a colorimetric lateral flow biosensor (LFB) for the visual detection of dengue-1 RNA using dextrin-capped gold nanoparticle (AuNP) as label. The detection was based on nucleic acid sandwich-type hybridization among AuNP-labeled DNA reporter probe, dengue-1 target RNA, and dengue-1 specific DNA capture probe immobilized on the nitrocellulose membrane. Positive test generated a red test line on the LFB strip, which enabled visual detection. The optimized biosensor has a cut-off value of 0.01 µM using synthetic dengue-1 target. Proof-of-concept application of the biosensor detected dengue-1 virus in pooled human sera with a cut-off value of 1.2 × 104 pfu/mL. The extracted viral RNA, when coupled with nucleic acid sequence-based amplification (NASBA), was detected on the LFB in 20 min. This study first demonstrates the applicability of dextrin-capped AuNP as label for lateral flow assay. The biosensor being developed provides a promising diagnostic platform for early detection of dengue infection in high-risk resource-limited areas. View Full-Text
Keywords: dengue-1; dextrin-capped AuNP; lateral flow biosensor; NASBA; sandwich-type hybridization dengue-1; dextrin-capped AuNP; lateral flow biosensor; NASBA; sandwich-type hybridization
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MDPI and ACS Style

Yrad, F.M.; Castañares, J.M.; Alocilja, E.C. Visual Detection of Dengue-1 RNA Using Gold Nanoparticle-Based Lateral Flow Biosensor. Diagnostics 2019, 9, 74.

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