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Open AccessArticle

The Effects of Low-Dose Irradiation on Human Saliva: A Surface-Enhanced Raman Spectroscopy Study

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Department of Orthodontics and Dentofacial Orthopedics, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400001 Cluj-Napoca, Romania
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Department of Oral Radiology, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania
3
MedFuture Research Center for Advanced Medicine, 400337 Cluj-Napoca, Romania
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Department of Oral and Maxillofacial Surgery, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400029 Cluj-Napoca, Romania
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Faculty of Physics, “Babeș-Bolyai” University, 400084 Cluj-Napoca, Romania
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Department of Prosthetic Dentistry and Dental Materials, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400006 Cluj Napoca, Romania
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Research Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania
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Department of Functional Genomics and Experimental Pathology, Oncological Institute “Prof. Dr. Ion Chiricuţă”, 400015 Cluj-Napoca, Romania
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Department of Pharmaceutical Physics-Biophysics, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania
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Department of Implantology and Maxillofacial Surgery, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400033 Cluj-Napoca, Romania
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Diagnostics 2019, 9(3), 101; https://doi.org/10.3390/diagnostics9030101
Received: 8 July 2019 / Revised: 7 August 2019 / Accepted: 21 August 2019 / Published: 22 August 2019
(This article belongs to the Collection Biomarkers in Medicine)
Biological effects of low-dose ionizing radiation (IR) have been unclear until now. Saliva, because of the ease of collection, could be valuable in studying low-dose IR effects by means of surface-enhanced Raman spectroscopy (SERS). The objective of our study was to compare the salivary SER spectra recorded before and after low-dose IR exposure in the case of pediatric patients (PP). Unstimulated saliva was collected from ten PP before and after irradiation with a cone beam computed tomography (CBCT) machine used for diagnostic purposes. The SERS measurements have been recorded on dried saliva samples using a solid nanosilver plasmonic substrate synthesized using an original method developed in our laboratory. The experimental results showed that salivary SER spectra are dominated by three vibrational bands (441,735 and 2107 cm−1) that can be assigned to bending and stretching vibrations of salivary thiocyanate (SCN-). After exposure, an immediate increase of vibrational bands assigned to SCN- has been recorded in the case of all samples, probably as a result of IR interaction with oral cavity. This finding suggests that SCN- could be used as a valuable biomarker for the detection and identification of low-dose radiation effects. View Full-Text
Keywords: ionizing radiation; pediatric patients; Raman spectroscopy; thiocyanate ionizing radiation; pediatric patients; Raman spectroscopy; thiocyanate
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Colceriu-Șimon, I.M.; Hedeșiu, M.; Toma, V.; Armencea, G.; Moldovan, A.; Știufiuc, G.; Culic, B.; Țărmure, V.; Dinu, C.; Berindan-Neagoe, I.; Știufiuc, R.I.; Băciuț, M. The Effects of Low-Dose Irradiation on Human Saliva: A Surface-Enhanced Raman Spectroscopy Study. Diagnostics 2019, 9, 101.

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